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All Journal ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Manilkara: Journal of Bioscience
Rumella Simarmata, Rumella
Pusat Penelitian Bioteknologi, LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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Amelioration of Salt Tolerance in Soybean (Glycine Max. L) by Plant-Growth Promoting Endophytic Bacteria Produce 1-Aminocyclopropane-1-Carboxylase Deaminase Simarmata, Rumella; Ngadiman, Ngadiman; Rohman, Saifur; Simanjuntak, Partomuan
ANNALES BOGORIENSES Vol 22, No 2 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2018.v22.n2.81-93

Abstract

     Salinity is a major abiotic stress that can induce ethylene synthesis beyond the normal limits as plants response to stress and hence reduces crop productivity. The 1-aminocyclopropane-1-carboxylase deaminase (ACCD)-producing bacteria can reduce excessive ethylene synthesis by taking ACC (ethylene precursor) as a nitrogen source. This study showed the possibility of using endophytic bacteria in order to reduce the undesirable effects of salinity. Strain Pseudomonas putida PIR3C and Roultella terrigena PCM8 exhibited promising performance for promoting the growth of plant under salinity stress conditions. The results showed that bacterial inoculation was effective even in the presence of higher salinity levels. Strain P. putida PIR3C was the most efficient strain compared to the other strains and significantly increased shoot length, root length, dry weight, germination percentage, and reduced stem diameter. The role of ACCD in reducing ethylene production under salinity stress conditions was also studied by measuring the evolution of ethylene in vitro by soybean seeds treated with some ACCD bacterial strain. The maximum ethylene lowering capacity was observed in R. terrigena PCM8, the strain reduced ethylene production from 622.81 nmol.g-1(control) to 352.78 nmol.g-1 (43% reduction). The production of α-ketobutyrate, chlorophyll content and germination percentage from P. putida PIR3C was higher than other strains. The results suggested that strain P. putida PIR3C and R. terrigena PCM8 can be employed for salinity tolerance in soybean seedlings and may have better prospects for an amelioration of stress condition.
Isolation, Screening and Identification of Plant Growth-Promoting Endophytic Bacteria from Theobroma cacao Simarmata, Rumella; Widowati, Tiwit; Dewi, Tirta Kumala; Lekatompessy, Sylvia J. R.; Antonius, Sarjiya
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.21280

Abstract

Cacao (Theobrama cacao) is one of the main plantation commodities in Indonesia which has an important role for the national economy. The low productivity of cacao plants in Indonesia is due to the condition of old plants, pests and diseases attacks which affect the quality of the fruit and decrease the plant’s productivity. The objective of this study was to isolate and identify of endophytic bacteria from stem, flower, leaf and fruit of T. cacao by using 16S rRNA gene as genetic marker. Twenty seven endophytic bacterial isolates were collected from local plantation in Yogyakarta area.  From this study, 8 endophytic bacterial strains exhibited the higher PGP traits. The isolates produced Indole Acetic Acid level by 0.3 to 5.21 ppm/hour. All of isolates had nitrogen fixation activity but have not phosphate solubilization activity. Among them, isolates CSDT 4 and CGKBH 4 showed promising potential as PGP bacteria. Based on the 16S rRNA gene sequences, those bacterial strains were identified as Brevibacillus brevis (CSDT 4) and Pantoea sp. (CGKBH 4). We propose that the B. brevis and Pantoea sp. which is reported for the first time for their PGP potential in cacao, exerts its beneficial effects on cacao crop through combined of activities. The potential PGP bacteria from the Cacao plant was used to make a specific bio-fertilizer formula for the Cacao plant, because of the different needs and condition that every plant requires.
Analisis Kemampuan Produksi Auksin dari Bakteri Endofit dan Plant Growth Promoting Rhizobacteria (PGPR) dalam Akar Cabai Merah Keriting (Capsicum annum L.) Maulida, Nurdalila; Rahmawati, Arrum; Dewi, Tirta Kumala; Simarmata, Rumella; Widowati, Tiwit; Kartika, Titik; Guswenrivo, Ikhsan; Nugroho, Deddy Triyono; Supriatna, Ateng
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9817.2024

Abstract

Endophytic bacteria found in the roots, stems, and leaves of plants have potential as biocontrol agents and drivers of plant development, besides that in plant roots there are also plant growth promoting rhizobacteria (PGPR) with the same ability. The consortium of the two bacteria will produce Indole 3 acetic acid (IAA) compounds which are then converted into auxins that are beneficial to plants. The research aims to determine the ability of endophytic bacteria and PGPR from the roots of curly red chili plants in producing auxin hormones. The research work procedure includes rejuvenation of bacterial isolates, macroscopic and microscopic characteristics, measuring auxin levels using HPLC (High Performance Liquid Chromatography). The results showed that the consortium of 6 bacteria (3 endophytes and 3 PGPR) produced an auxin concentration of 46.122 ppm, a consortium of endophytic bacteria of 24.201 ppm, a consortium of PGPR bacteria of 162.723 ppm, and finally single endophytic bacteria and single PGPR bacteria produced auxin of A 158.913 ppm; B 64.882 ppm; C 93.923 ppm; CB1 240.817 ppm; CB2 186.807 ppm; and CMBC 11.689 ppm, while for the control 0 ppm. In conclusion, the highest auxin concentration was obtained from the single result of PGPR bacteria CB1 240.817 ppm.
Co-Authors Ateng Supriatna Dewi, Tirta Kumala Guswenrivo, Ikhsan Kartika, Titik Lekatompessy, Sylvia J. R. Maulida, Nurdalila Ngadiman Ngadiman Nugroho, Deddy Triyono Partomuan Simanjuntak Rahmawati, Arrum Saifur Rohman Sarjiya Antonius Tiwit Widowati, Tiwit