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Steroids from Atactodea striata and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines Evan Hadrian; Aprilia Permata Sari; Tri Mayanti; Desi Harneti; Rani Maharani; Darwati Darwati; Kindi Farabi; Unang Supratman; Celcius Waranmaselembun; Supriatno Salam; Sofa Fajriah; Muhamad Nurul Azmi
Indonesian Journal of Chemistry Vol 23, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.76438

Marine environment is known as a source of potential steroids with multiple biological activities, one of which is an anticancer agent. Atactodea striata are one of the seashells distributed in Indonesia located in the Kei Islands, Southeast Maluku. During the course of our continuing search for biologically active substances from Indonesia seashells, seven steroids have been isolated from the n-hexane fraction of A. striata and they were identified as 7β-hydroxy-sitosterol (1), campesterol (2), β-sitosterol (3), cholesterol (4), 5α,8α-epidioxycholest-6-en-3-β-ol (5), 7-keto-cholesterol (6), and 7α-hydroxy-cholesterol (7). The structure was identified by spectroscopic methods including 2D NMR techniques, FTIR, HRTOFMS, and chemical shift comparison with previously reported spectral data. Compounds 1-7 were evaluated for their cytotoxic effects against MCF-7 breast cancer cells and showed weak or no anticancer activity.

NF-κB and TNF-α Expressions and Histological Image of White Rat’s, Rattus norvegicus, Ileum With Indomethacine-Induced IBD (Inflammatory Bowel Desease) After Mas Ngur Oyster (Atactodea striata) Extract Therapy Dullah Irwan Latar; Celcius Waranmaselembun; Yuliana Anastasia Ngamel; Abu Samad Serang; Anna Kartika Ngamel
Eduvest - Journal of Universal Studies Vol. 3 No. 3 (2023): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v3i3.763

Inflammatory disease in gastric tract called Inflammatory Bowel Disease (IBD), particularly colon, in general, results from the use of non-steroid anti-inflammatory drug, such as indomethacine. The use of natural material, such as mas ngur oyster (Atactodea striata) as traditional medicine has been long known by people in Kei Islands–Southeast Mallucas. This study is aimed at measuring the ability of active compounds of this oyster extract in reducing NF-κB and TNF-α expression and showing the histological image of indomethacine-induced rat ileum with IBD after treatment with mas ngur oyster extract. It used 8-12 week old male rats (Rattus norvegicus) of 150 - 200 g BW. The rats were divided into 3 groups, healthy group, sick group (induced with 15 mg/kg BW of indomethacine), and treatment group (orally induced with indomethacine at a dose of 15 mg/kg BW then treated with mas ngur oyster extract at the dose of 100, 400, 700 mg/kg BW). Indomethacine induction at the dose of 15 mg/kg BW and mas ngur oyster extract therapy were orally administered. NF-κB and TNF-α expressions were measured using immunohistochemicals, and the histological image used Hematoksilin-Eosin staining. Results showed that extract therapy gave significant effect (P<0.05) at the effective dose of 400 mg/kg BW that could reduce 86.421% of NF-κB expression and 60.972% of TNF-α expression in the rat ileum and result in tissue recovery of the IBD rat’s ileum after the therapy.

NF-κB and TNF-α Expressions and Histological Image of White Rat’s, Rattus norvegicus, Ileum With Indomethacine-Induced IBD (Inflammatory Bowel Desease) After Mas Ngur Oyster (Atactodea striata) Extract Therapy Dullah Irwan Latar; Celcius Waranmaselembun; Yuliana Anastasia Ngamel; Abu Samad Serang; Anna Kartika Ngamel
Eduvest - Journal of Universal Studies Vol. 3 No. 3 (2023): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v3i3.763

Inflammatory disease in gastric tract called Inflammatory Bowel Disease (IBD), particularly colon, in general, results from the use of non-steroid anti-inflammatory drug, such as indomethacine. The use of natural material, such as mas ngur oyster (Atactodea striata) as traditional medicine has been long known by people in Kei Islands–Southeast Mallucas. This study is aimed at measuring the ability of active compounds of this oyster extract in reducing NF-κB and TNF-α expression and showing the histological image of indomethacine-induced rat ileum with IBD after treatment with mas ngur oyster extract. It used 8-12 week old male rats (Rattus norvegicus) of 150 - 200 g BW. The rats were divided into 3 groups, healthy group, sick group (induced with 15 mg/kg BW of indomethacine), and treatment group (orally induced with indomethacine at a dose of 15 mg/kg BW then treated with mas ngur oyster extract at the dose of 100, 400, 700 mg/kg BW). Indomethacine induction at the dose of 15 mg/kg BW and mas ngur oyster extract therapy were orally administered. NF-κB and TNF-α expressions were measured using immunohistochemicals, and the histological image used Hematoksilin-Eosin staining. Results showed that extract therapy gave significant effect (P<0.05) at the effective dose of 400 mg/kg BW that could reduce 86.421% of NF-κB expression and 60.972% of TNF-α expression in the rat ileum and result in tissue recovery of the IBD rat’s ileum after the therapy.

Malondialdehyde (MDA) Concentration and Histopathological Image of Indomethacine-Induced Wistar Rat,Rattus norvegicus,With Inflammatory Bowel Disease (IBD) After Mas Ngur Oyster (Atactodea striata) Extract Therapy Celcius Waranmaselembun; Agustiana Agustiana; Firlianty Firlianty; Yuliana Anastasia Ngamel; Silvester B. Pratasik
Eduvest - Journal of Universal Studies Vol. 3 No. 3 (2023): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v3i3.764

Inflammatory Bowel Disease (IBD) is an inflammatory disease occurring in gastirct tract, particularly colon, that could result from the side effect of anti-inflammatory non-steroid(AINS) drug utilization, such asindomethacine. Mas ngur oysters (Atactodea striata) have long been known by people of Kei-Southeast Mallucas as traditional medicine, but its use in reducing MDA level in rat with IBD has not been studied. This study was aimed at measuring the ability of the bioactive compounds contained in mas ngur oyster powder extract to reduce the MDA level in the ileum of indomethacine-induced rat with IBD and providing the histological image of the ileum after therapized with mas ngur oyster extract. Test animals were 8-12 week old-male rats (Rattus norvegicus) of 150 - 200 g BW. They were separated into 3 groups,healthy, sick (induced with 15 mg/kg BW indomethacine), therapy goups (15 mg/kg BW indomethacine oral induction then treated with mas ngur oyster powder of100, 400, 700 mg/kg BW). Indomethacine induction of 15 mg/kg BWand therapy of mas ngur oyster powder extract were administered orally. MDA level was measured usingThiobarbituric Acid (TBA)test and histological image of the ileum using Hematoxylin-Eosin staining. Results showed that mas ngur extract therapy gave significant effectin MDA level (P<0.05) and difference between the treatments with effective dose of400 mg/kg BW

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