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Potensi Puding Ikan Toman (Channa micropeltes) dan Ikan Gabus (Channa striata) untuk Percepatan Penyembuhan pada Hewan Uji Tikus Firlianty, Firlianty; Pratasik, Silvester B.
Agrikan: Jurnal Agribisnis Perikanan Vol 11, No 2 (2018)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.6 KB) | DOI: 10.29239/j.agrikan.11.2.65-69

Abstract

Everyone has different activities with different risks and often gets injured. Since wound healing process takes time, better handling and alternative medicinal administration are needed to accelerate the health recovery from trauma caused either by sharp or hard materials.  Fish are high nutritive food enriched with minerals, fat, and protein. This study was intended to know the effect of snakehead fish extract on the healing process of white rat (Rattus novergicus). It applied an experimental method with 3 treatments. Results showed that fish pudding added with the snakehead fish extract gave good effect on the acceleration of wound healing process in the white rat.
ESTIMASI KANDUNGAN KARBON (C) PADA SERASAH DAUN MANGROVE DI DESA LANSA, KECAMATAN WORI, KABUPATEN MINAHASA UTARA Tidore, Fadli; Rumengan, Antonius; Sondak, Calvyn F.A.; Mangindaan, Remy E.P.; Runtuwene, Heard C.C.; Pratasik, Silvester B.
JURNAL PESISIR DAN LAUT TROPIS Vol 6, No 2 (2018): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.6.2.2018.21529

Abstract

Global warming is one of the environmental issues related to climate change. Coastal blue carbon ecosystems such as mangrove and seagrass have ability to combat global warming. Mangrove ecosystem has an important ecological function in efforts to mitigate global warming, by carbon storage. This study was done in Lansa Village mangrove forest and focused on mangrove leaf litter. The purpose of this study was to estimate carbon content in mangrove leaf litter. Leaf litter samples were collected by using a 1x1 m2 litter trap, which was made of black nylon with a mesh size of about 0.2 cm, 8 traps were put under mangrove trees canopy, with a height of 1.5 m above sea level or at the highest tide. The samples were taken and observed every 7 days. The samples were analyzed by using Dry-Ash Method. The results showed that the average litter production of gram wet weight (Gbb) and gram dry weight obtained during the study were 122.97 gbb m2/28 days, 4.39 gbb m2/28 days, 47.69 gbk m2/28 days, 1.83 gb m2/day. The average of mangrove leaf litter biomass is 30.12 g m2. The highest amount of carbon storage in mangrove litter was 19.30 gram C. The average value of the percentage of carbon content of all plots was 31.38% per day. Based on these results the estimated amount of carbon removal in mangrove leaves was 2.16 t C ha-1 y-1 or 337.18 t C y-1.and 1,237.45 t CO2 y-1.Ekosistem mangrove memiliki fungsi ekologis yang sangat penting dalam upaya mitigasi pemanasan global, yakni sebagai penyerap dan penyimpan karbon Hutan mangrove juga memiliki peran sebagai penyerap karbon dioksida (CO2) dari udara sehingga sangat berguna untuk mitigasi perubahan iklim. Tujuan dari penelitian ini adalah untung mengestimasi kandungan karbon pada serasah daun mangrove di hutan mangrove Desa Lansa, Kecamatan Wori, Kabupaten Minahasa Utara. penelitian ini dilakukan menggunakan metode litter trap yang berukuran 1x1 m2, yang terbuat dari nylon berwarna hitam dengan ukuran mata jaring (mesh size) sekitar 0,2 cm, sebanyak 8 buah dipasang di bawah kanopi pohon mangrove, dengan ketinggian 1,5 m di atas permukaan air laut atau pada pasang tertinggi untuk menampung jatuhnya serasah dan diamati setiap 7 hari. Hasil penelitian diperoleh rata-rata produksi serasah gram berat basah (Gbb) dan gram berat kering yang didapat selama penelitian, sebesar 122,97 gbb/m2/28hr, 4,39 gbb/m2/hr, 47,69 gbk/m2/28hr, 1,83 gbk/m2/hr. rata-rata biomassa serasah daun mangrove sebesar 41,07. Jumlah simpanan karbon tertinggi pada serasah mangrove sebesar 19,30 gram C. Nilai rata-rata persentase kandungan karbon dari semua plot adalah sebesar 31,38% per hari, berdasarkan hasil penelitian jumlah estimasi kandungan karbon yang tersimpan pada serasah daun mangrove 2,16 ton/ha/tahun.
Substansi anti bakteri dari jamur endofit pada mangrove Avicennia marina Ramadan, Febrian; Bara, Robert; Losung, Fitje; Mangindaan, Remy; Warouw, Veibe; Pratasik, Silvester
JURNAL PESISIR DAN LAUT TROPIS Vol 6, No 1 (2018): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.6.1.2018.20115

Abstract

Mangrove merupakan suatu  tumbuhan yang kaya akan senyawa bioaktif. Senyawa bioaktif yang terdapat dalam bagian-bagian mangrove tidak selalu berasal dari tanaman mangrove itu sendiri, melainkan  dari organisme lain yang mensintesis senyawa bioaktif di dalam bagian mangrove.  Dengan demikian ada kemungkinan terdapat jamur atau bakteri endofit yang hidup atau tinggal di tumbuhan mangrove dan berperan sebagai penghasil bioaktif yang sebenarnya.  Telah diisolasi enam isolat jamur endofit asal mangrove Avicennia marina yang tumbuh di Peariran Pantai tasik Ria, kabupaten Minahasa. Pengujian aktivitas antibakteri dalam penelitian ini menggunakan metode Kirby-Bauer yang dimodifikasi. Hasil yang diperoleh dalam penelitian ini terdapat isolat jamur endofit dari mangrove Avicennia marina.  Hanya 2 isolat jamur endofit yaitu A.M.D. 1.1.1. dan 1.1.2. yang memiliki aktivitas antibakteri terhadap bakteri B. megaterium dan E. coli.  Analisis mikroskopik dari kedua isolat berdasarkan bentuk miselium mengindikasikan kedua isolat  ini yaitu Aspergillus cf. flavus dan Talaromyces sp. Ekstrak kedua isolat jamur kemudian fraksinasi dan  diperoleh 3 fraksi yaitu fraksi air, etanol dan n-heksan.  Dari ketiga fraksi semuanya memiliki aktivitas antibakteri.  Hal ini menunjukkan bahwa senyawa dari isolat jamur endofit bersifat polar,semi polar dan non polar.  Hasil spektrofotometri menunjukkan bahwa senyawa antibakteri dari isolat jamur strain Aspergillus cf. flavus dan Talaromyces spmerupakan senyawa yang berbeda. Fraksi aktif dari strain Aspergillus cf. flavus dan Talaromyces spterdapat pada fraksi n-heksan, etanol dan air. Ketiga fraksi ini memiliki perbedaan pola serapan UV
POTENSI JENIS-JENIS IKAN AIR TAWAR KONSUMSI MASYARAKAT ALIRAN SUNGAI DIGOEL, KABUPATEN BOVEN DIGOEL, PAPUA, DAN BEBERAPA LANGKAH PENGELOLAANNYA Rarung, Lexy K; Pratasik, Silvester B
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol 6, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.571 KB) | DOI: 10.35800/jpkt.6.1.2010.116

Abstract

This study aimed at providing information on freshwater fish living in Digoel River, Boven Digoel Regency, Papua, edible for local people and some management options. Data were collected from observing the fishing activities and Tanah Merah  market, and through personal interviews as well. Results showed that there were about 23 species of native and introduced fish living in the Digoel River. Native fish population is decreasing as a result of forest clear cut, pollution, irrational fishing and predation. Therefore, management actions should be taken.
Tingkat kelangsungan hidup Trepang Benang (Bohadschia marmorta) dengan metode transverse-fission di perairan alam dan pada wadah terkontrol Rondonuwu, Yonatan Y.; Sambali, Hariyani; Sinjal, Hengky J.; Kusen, Diane J.; Kalesaran, Ockstan Y.; Pratasik, Silvester B.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.39261

Abstract

This research was conducted to examine the survival and regeneration success rate of the Brown Sandfish (Bohadchia marmorata) using the Transverse-fission method in containers placed in nature and controlled. This research was conducted  from June to July 2021, in Bahoi Village, West Likupang, North Minahasa. This research used the Transverse-fission method, in which B. marmorata was tied using a rubber band until cleavage occurred. The container used is a basket covered with a net as many as 6 units, each of which is insulated in the middle so that it becomes 12 parts. Individuals who have been tied, then observed for changes shortly after the binding is done until the individual is separated (every 1 - 2 hours), followed by observing the survival and regeneration of separate body parts (every 6 hours). The results of the study were then analyzed using SPSS 22 version with T-test.  Based on the results of the research, the survival and regeneration success rate of B. marmorata using the Transverse-fission method placed in containers in natural waters was significantly different from that of B. marmorata placed in controlled containers, where the total survival rate of B. marmorata placed in in controlled containers reached 100% while the total survival rate of B. marmorata placed in natural waters only reached 3.33%. 
Characteristics of Gel Emulsion Formulation of Snakehead (Channa Micropeltes) As Wound Healer Firlianty Firlianty; Choirul Anwar; Anang Najamuddin; Silvester B. Pratasik
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.642 KB) | DOI: 10.20884/1.oa.2018.14.2.545

Abstract

This study is aimed to know the characteristics and the effectivity of gel emulsion of snakehead (Channa micropeltes) extract in accelerating wound healing process. It used experimental method with Complete Randomized Design. Four treatments with 3 replications were employed, i.e. 1%, 2%, and 4% gel emulsion of snakehead Channa micropeltes extract and a control treatment. Results showed that C. micropeltes extract formulated in gel emulsion had white colour, typical aroma of gel emulsion, soft texture with mean dispersion ability of 3 cm, and pH 6. In vivo test on male mices that gel emulsion of C. micropeltes extract was highly effective (P≤0.05) to accelerate wound healing, in which the treatment of 1% gel emulsion gave the highest effect with 98.5% wound healing at day-14, followed with that of control treatment, 95% healing, then 2%, 90% healing and 4%, 90% healing, respectively. Snakehead (Channa micrpeltes) extract formulated in gel emulsion can speed up the wound healing process.
Community Structure And Condition Of Coral Reefs In Poopoh Village Waters, Tombariri District, Minahasa Regency Marselo R. Manzanaris; Ari B. Rondonuwu; Silvester B. Pratasik
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.21439

Abstract

This study aims to provide information about coral reefs condition in Poopoh waters and as input For related stakeholders for future coral reef management and conservation. Data sampling employed SCUBA gear with Line Intercept Transect (LIT) at 3 M and 10 M depth. It used 30 M-transect line and each depth was laid 3 transects.Results showed that the highest number of hard coral colonies at 3 M depth was found in branching coral and the lowest in ACD, while at 10 M depth, the highest number of colonies was recorded in CMR and the lowest in ACB.  Based on percent cover, it was found that the depth of 3 M had moderate coral condition, while the depth of 10 M had poor condition. Both depths had low diversity index, high eveneness index, and low dominance index.Key words: coral reef condition,  percent cover, diversity, evenness, dominance.  ABSTRAKPenelitian ini bertujuan untuk memberikan informasi tentang kondisi terumbu karang di perairan Desa Poopoh sebagai masukan bagi pihak-pihak terkait untuk pengelolaan dan pelestarian terumbu karang di masa mendatang.Pengambilan data terumbu karang menggunakan alat SCUBA dengan metode LIT (Line Intercept Transek) pada kedalaman 3 M dan 10 M. Penelitian in menggunakan 30 M panjang  transek, dan masing-masing kedalaman ditempatkan 3 transek.Jumlah koloni karang batu terbanyak di kedalaman 3 meter ditemukan pada jenis karang bercabang (CB), 4,33 koloni dan jumlah koloni terendah pada ACD, sedangkan pada kedalaman 10 M, koloni terbanyak ditemukan CMR dan terendah pada ACB.  Berdasarkan persentase tutupan, ditemukan bahwa kedalaman 3 meter memiliki kondisi terumbu karang sedang, sedangkan kedalaman 10 M memiliki kondisi terumbu karang buruk. Kedua kedalaman memiliki indeks keanekaragaman (H’) rendah, indeks kemerataan tinggi, dan indeks dominasi tergolong rendah.Kata kunci: kondisi terumbu karang, persen tutupan, keragaman, pemerataan, dominasi.
Fly fish growth pattern (Decapterus spp) in Likupang Water, Nort Sulawesi Inggrid M. F Akerina; Silvester B. Pratasik; Nego E. Bataragoa
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.22593

Abstract

This study was aimed to find out the size distribution of scad (Decapterus spp) caught by Likupang fishermen and to find out theirgrowth patterns. Fish samples were collected by traditional purse seine fishermen in Likupang waters. Total catches were 139 individuals consisting of 89 D. macarellus and 50 D. macrosoma.The former was dominated by size of 19.2-20.6 cm, while the latter was dominated by size of 13.1-14.1 cm. Length-weight relationship analysis showed that the growth patterns of D. macarellus and D. Macrosoma were isometric meaning that the increase in length is consistent with weight gain.Keywords: Likupang, Decapterus spp and growth patternsABSTRAKPenelitian ini bertujuan untuk mengetahui distribusi ukuran ikan layang (Decapterus spp) yang ditangkap oleh nelayan Likupang and untuk menduga pola pertumbuhannya. Sampel ikan diperoleh nelayan tradisional pukat cincin di perairan Likupang.Total hasil tangkapan adalah 139 ekor terdiri dari 89 ekor  D. macarellus dan 50 D. macrosoma. Hasil tangkapan D. macarellus didominasi oleh kelas ukuran 19,2-20,6 cm, sedangkan D. macrosomadidominasi oleh kelas ukuran 13,1-14,1 cm. Analisis hubungan panjang berat menunjukkan bahwa pola pertumbuhan ikan D. macarellusdan D. macrosoma bersifat isometrik yang artinya pertambahan panjang selaras dengan pertambahan berat.Kata kunci: Likupang, Decapterus spp dan Pola pertumbuhan
Marine Debris Composition on Tasik Ria Beach, Tombariri, Minahasa Regency Silvia A. Bangun; Joudy R.R. Sangari; Frans F. Tilaar; Silvester B. Pratasik; Meiske S. Salaki; Wilmy Pelle
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23411

Abstract

Indonesia is referred to as the second largest contributor of marine plastic waste in the world after China, with an estimated 0.48-1.29 million metric tons per year (Jambeck et al, 2015). The main problem today is the lack of information about pollution of marine debris on the coast, especially in North Sulawesi. This study aims to identify the type of marine debris on Tasik Ria Beach using the transect line observation method. Observation of marine debris was carried out 5 times with a total of 10 transects between February and April 2019. Data analysis was carried out using several software namely Microsoft Excel, Statgraphics, and JMP. The analysis technique used is EDA (Exploratory Data Analysis) with GDA (Graphical Data Analysis) as the main approach. Of the various types of debris obtained, plastic debris is the most commonly found, as many as 189 items, followed by glass 97 items, wood and derivatives of 11 items, rubber 5 items and clothes 2 items. Based on the results of the study, the type of macro debris is the most common category of debris at the study site. The total number of macro-debris collected in ten observation transects was 316 items with a total weight of 118.62 gr/m2, while meso-debris had only 6 items with a total weight of 7.18 gr/m2. The percentage of macro-debris composition found on Tasik Ria beach is plastic (58.15%), glass (29.85%), metal (6.52%), wood and derivatives (3.42%), rubber (1, 55%) and clothes (0.62%). These results can illustrate the potential for events where plastic is the dominant component of marine debris on the coast, specifically in the District of Tombariri, Minahasa Regency.Keywords: Marine debris, Macro-debris, Category, Composition, Tasik Ria ABSTRAKIndonesia disebut sebagai kontributor sampah plastik ke laut terbesar kedua di dunia setelah Tiongkok, dengan estimasi 0,48-1,29 juta metrik ton per tahun (Jambeck et al, 2015). Masalah utama dewasa ini adalah kurangnya informasi mengenai pencemaran sampah laut di pantai, khususnya di Sulawesi Utara. Penelitian ini bertujuan untuk mengidentifikasi jenis sampah laut di Pantai Tasik Ria dengan menggunakan metode pengamatan garis transek. Pengamatan sampah laut dilakukan sebanyak 5 kali dengan total 10 transek antara bulan Februari hingga April 2019. Analisis data dilakukan dengan menggunakan perangkat lunak yaitu Microsoft Excel, Statgraphics, dan JMP. Adapun tehnik analisis yang digunakan adalah EDA (Exploratory Data Analysis) dengan pendekatan utama yaitu, GDA (Graphical Data Analysis). Dari berbagai semua jenis sampah yang didapatkan, sampah plastik merupakan yang paling banyak ditemukan yaitu sebanyak 189 item, diikuti kaca 97 item, kayu dan turunannya 11 item, karet 5 item dan terakhir pakaian 2 item. Berdasarkan hasil penelitian, jenis sampah makro merupakan ukuran sampah yang paling banyak ditemukan di lokasi penelitian. Jumlah total makro-debris yang dikumpulkan di sepuluh transek pengamatan adalah sebanyak 316 item dengan bobot total 118,62 gr/m2, sedangkan meso-debris hanya terdapat 6 item dengan bobot total 7,18 gr/m2. Persentase komposisi makro-debris yang terdapat di pantai Tasik Ria adalah plastik (58,15%), kaca (29,85%), logam (6,52%), kayu dan turunannya (3,42%), karet (1,55%) dan pakaian (0,62%). Hasil ini dapat menggambarkan potensi kejadian dimana plastik menjadi komponen sampah laut dominan di pantai, secara khusus di Kecamatan Tombariri, Kabupaten Minahasa.Kata kunci: Sampah laut, Makro-debris, Jenis, Komposisi, Tasik-Ria
Malondialdehyde (MDA) Concentration and Histopathological Image of Indomethacine-Induced Wistar Rat,Rattus norvegicus,With Inflammatory Bowel Disease (IBD) After Mas Ngur Oyster (Atactodea striata) Extract Therapy Celcius Waranmaselembun; Agustiana Agustiana; Firlianty Firlianty; Yuliana Anastasia Ngamel; Silvester B. Pratasik
Eduvest - Journal of Universal Studies Vol. 3 No. 3 (2023): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v3i3.764

Abstract

Inflammatory Bowel Disease (IBD) is an inflammatory disease occurring in gastirct tract, particularly colon, that could result from the side effect of anti-inflammatory non-steroid(AINS) drug utilization, such asindomethacine. Mas ngur oysters (Atactodea striata) have long been known by people of Kei-Southeast Mallucas as traditional medicine, but its use in reducing MDA level in rat with IBD has not been studied. This study was aimed at measuring the ability of the bioactive compounds contained in mas ngur oyster powder extract to reduce the MDA level in the ileum of indomethacine-induced rat with IBD and providing the histological image of the ileum after therapized with mas ngur oyster extract. Test animals were 8-12 week old-male rats (Rattus norvegicus) of 150 - 200 g BW. They were separated into 3 groups,healthy, sick (induced with 15 mg/kg BW indomethacine), therapy goups (15 mg/kg BW indomethacine oral induction then treated with mas ngur oyster powder of100, 400, 700 mg/kg BW). Indomethacine induction of 15 mg/kg BWand therapy of mas ngur oyster powder extract were administered orally. MDA level was measured usingThiobarbituric Acid (TBA)test and histological image of the ileum using Hematoxylin-Eosin staining. Results showed that mas ngur extract therapy gave significant effectin MDA level (P<0.05) and difference between the treatments with effective dose of400 mg/kg BW
Co-Authors Adnan Wantasen Agustiana Agustiana Alex D. Kambey Ali Djamhuri Ali, Fajri Nurul Anang Najamuddin Anneke V. Lohoo Antonius Rumengan Ari B. Rondonuwu Bataragoa, M.Sc, Dr. Ir. Nego E. Billy Th. Wagey Calvyn F. A. Sondak, Calvyn F. A. Celcius Waranmaselembun Choirul Anwar Darus S. Paransa Dauhan, Dulce Maria Deiske A. Sumilat Deslie RH Kumampung Elvy L. Ginting Erly Y Kaligis Erly Y. Kaligis, Erly Y. Firlianty Firlianty Firlianty Firlianty Firlianty, Firlianty Firlianty, Firlianty Fitje Losung Frans F. Tilaar Fransine B. Manginsela Gaspar D. Manu, Gaspar D. Gaspar Manu Ginting, Elvy Like Gustaf F. Mamangkey Hengky J. Sinjal, Hengky J. Inggrid M. F Akerina Janny D. Kusen John L. Tombokan Joshian NW Schaduw Joudy R.R. Sangari Juliaan Watung Kalesaran, Ockstan Y. Kondoy, Khiristin Ivone Fisye Lalamentik, Laurentius Th. X. Laura, Azhar Lawrence J. L. Lumingas Leleran, Andreas J. P. L. Lexy K Rarung Malurung, Yulianti - Mamonto, Riswanto Mangindaan, Remy Mantiri, Nicola R. K. Marselo R. Manzanaris Medy Ompi Medy Ompi Meiske S. Salaki Melani, Hellen Menajang, Febry S. I. Menajang, Febry Susana Ivone Moningkey, Ruddy D. Natalie D Rumampuk Nego E. Bataragoa Nego E. Bataragoa, Nego E. Novia A Jambo Pelle, Wilmy E. Purniasih, Ni Komang Pitri Ramadan, Febrian Rangan, Jety K. Rembet, Unstain Remy E. P Mangindaan Rene Charles Kepel, Rene Charles Robert A. Bara Rondonuwu, Arie B. Rondonuwu, Yonatan Y. Runtuwene, Heard C.C. Rustikasari, Irna Salaki, Meiske S. Sambali, Hariyani Sampe, Ayumi Angraini Sampouw, Mouren V. Samuel L. Opa Sangari, Joudy R.R Silvia A. Bangun Sinjal, Henky Stenly Wullur Suria Darwisito Suzanne L Undap Tidore, Fadli Tilaar, Ferdinand F. Tombi, Indra Unstain N. W. J. Rembet Veibe Warouw Wewengkang, Fabiola Wilmy Pelle Yuliana Anastasia Ngamel