Nukhrawi Nawir
Hasanuddin University

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Analysis of Changes in Malondialdehyde (MDA) and Lactate Dehydrogenase (LDH) Levels After a 30 km Cycling Event in The Makassar Cycling Community Andi Eka Nur Wahyu; Irfan Idris; Djohan Aras; Andi Wardihan Sinrang; Ilhamjaya Patellongi; Nukhrawi Nawir
COMPETITOR: Jurnal Pendidikan Kepelatihan Olahraga Vol 15, No 1 (2023): February
Publisher : UNIVERSITAS NEGERI MAKASSAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/cjpko.v15i1.43630

Abstract

This study looks at the changes in MDA and LDH levels after a 30 km cycling event in the Makassar cycling community. This research is a pre-experimental with a one-group pretest-posttest design on 30 male cyclists aged 30-60 years. The pre-test was carried out the day before the cycling event, and then the post-test was carried out 2 hours after the 30 km cycling event. Blood samples were tested using the ELISA Kit. Data were analyzed using the Wilcoxon test to see the effect of a 30 km cycling event on changes in MDA and LDH levels. Spearman correlation test to see the correlation between changes in MDA levels and LDH. The results of the study found that the 30 km cycling event had a significant effect on changes in MDA levels due to lipid peroxidation (p<0.05) and a significant effect on changes in LDH levels due to increased lactate secretion (p<0.05). There was no significant correlation between changes in MDA and LDH levels (p=0.60) with a negative correlation, which means that the more significant the change in MDA levels, the lower the LDH level.
Analysis Of Changes In Nitric Oxide (NO) And F2-Isoprostane Levels After The 30 Km Cycling Event In The Makassar Bike Community Risna Yunita; Ilhamjaya Pattelongi; Andi Ariyandy; Andi Wardihan Sinrang; Djohan Aras; Nukhrawi Nawir
COMPETITOR: Jurnal Pendidikan Kepelatihan Olahraga Vol 15, No 1 (2023): February
Publisher : UNIVERSITAS NEGERI MAKASSAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/cjpko.v15i1.43631

Abstract

This study aims to determine changes in Nitric Oxide (NO) and F2-Isoprostane levels after a 30 km cycling event in the Makassar cycling community. This research was a pre-experimental study with a one-group pretest & post-test design with 30 cyclists as subjects in the city of Makassar, men aged 30-60 years. Blood sampling was carried out pre-event and post-event cycling as far as 30 Km. Measurement of F2-Isoprostane & Nitric Oxide levels using the ELISA method. Data analysis used the Wilcoxon test to see changes in Nitric Oxide (NO)  and F2-Isoprostane levels after participating in a 30 Km cycling event. Data analysis using the Mann-Whitney test was used to see differences in changes in Nitric Oxide (NO)  and F2-Isoprostane levels in the age group, average speed, exercise category, BMI, and smokers. Spearman test to see the correlation of changes in Nitric Oxide (NO)  and F2-Isoprostane levels. The results of this study found that the 30 km cycling event had a significant effect on changes in Nitric Oxide (NO)  levels which could improve the function of the vascular endothelium with p=0.0001. Meanwhile, the 30 km cycling event had no significant effect on changes in F2-Isoprostane levels, which meant that it had no risk of oxidative stress and tissue damage (p=0.688). There is no positive correlation between changes in Nitric Oxide (NO)  levels and changes in F2-Isoprostane levels, which means that changes in the repair of vascular endothelial function seem to suppress free radical production which leads to oxidative stress after cycling as far as 30 Km.