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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis JURNAL AGRONIDA
Kartiman, Roni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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MULTIPLIKASI IN VITRO ANGGREK HITAM (Coelogyne pandurata Lindl.) PADA PERLAKUAN KOMBINASI NAA DAN BAP Kartiman, Roni; Sukma, Dewi; Aisyah, Syarifah Iis; Purwito, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1006.134 KB) | DOI: 10.29122/jbbi.v5i1.2908

Abstract

In Vitro Multiplication of  Black Orchid (Coelogyne pandurata Lindl.) Using the Combination of NAA and BAPABSTRACTBlack orchid is an indigenous plant from Kalimantan, Indonesia. It becomes endangered because of forest over-exploitation and its low natural reproduction rate. Tissue culture is considered to offer a solution to conserve and propagate this species. The aim of this research was to evaluate the effect of Naphtalene Acetic Acid (NAA) and 6-Benzile Amino Purine (BAP) on shoots multiplication of black orchid. The basic medium used was a half of Murashige & Skoog (MS) composition supplemented with 150 mLL-1 coconut water. Initial explants used were 6-month-old shoots of germinating seeds. The shoot cultures were incubated for 23 weeks. Results showed that the best combination for shoot multiplication was NAA 0.0 mgL-1 with BAP 0.2 mgL-1. Shoot grew better on medium with BAP and without NAA while roots growth was better on medium without the two plant growth regulators. The addition of BAP up to 0.3 mgL-1 increased the leaf number, which however decreased at higher BAP concentration.Keywords: BAP, black orchid, Coelogyne pandurata, multiplication, NAA ABSTRAKAnggrek hitam merupakan flora langka asli Kalimantan, Indonesia. Keberadaa anggrek ini di alam semakin langka akibat eksploitasi berlebihan dan sulitnya perbanyakan secara alami. Kultur jaringan merupakan metode untuk mengatasi kelangkaan anggrek ini. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap multiplikasi anggrek hitam. Media dasar yang digunakan adalah ½ MS dengan penambahan air kelapa 150 mLL-1. Eksplan yang digunakan adalah tunas hasil semai biji umur 6 bulan. Kultur tunas diinkubasi selama 23 minggu. Hasil penelitian menunjukkan bahwa kombinasi terbaik untuk multiplikasi tunas adalah NAA 0 mgL-1 dengan BAP 0,2 mgL-1. Tunas tumbuh lebih baik dalam media dengan penambahan BAP tanpa NAA, sedangkan akar pada media tanpa NAA dan BAP. Penambahan BAP sampai 0.3 mgL-1 mampu meningkatkan jumlah daun, namun menurun dengan penambahan di atas konsentrasi tersebut.Kata Kunci: anggrek hitam, BAP, Coelogyne pandurata, multiplikasi, NAA
The Effect of Concentration and Immersion Time Disinfectant on Sterilization Aglaonema Hybrid (Pink Katrina) Leaves Budiarni, Kasandra; Hindaningrum, Irni Furnawanthi; Muharromah, Restu Siwi; Kartiman, Roni; Novita, Linda; Hardiani, Lany; Elya, Mardoni; Kubil
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7632

Abstract

Aglaonema sp. is well known as an ornamental plant in Indonesian society. It has leaf with various beautiful patterns. Aglaonema pink katrina is one of the imported hybrids Aglaonema. A method to increase plant yield quickly is tissue culture. The crucial step in plant tissue culture is sterilization. This study aimed to find the best sterilization method by looking at the effect of concentration and immersion time of sodium hypochlorite (NaOCl) as disinfectant to reduce contaminants explant Aglaonema Pink Katrina leaves. In this study, there were 11 groups consisting of positive control, negative control, and immersion with 2,5; 5%; and 10% NaOCl for 1 minute and 3 minutes. Parameters observed included the time of first contamination, percentage of bacterial and fungal contamination, and appearance of explants for 28 days. The DMRT test showed significant differences in all treatments of disinfectant concentration and soaking time.The results show contamination first time occurred in code S0a (negative control), the highest percentage of sterile explants was in code A5, and explants that were still green/fresh were found in codes A5 and A8 after observation.
Pengaruh intensitas naungan terhadap morfologi dan warna daun Aglaonema commutatum ‘Siam Aurora’ Afifah, Ulil Azmi Nurlaili; Rusmiyati, Henny; Kartiman, Roni; Manalu, Doni Sahat Tua; Tirtana, Arif; Marithasari, Harries
JURNAL AGRONIDA Vol. 11 No. 1 (2025): Jurnal Agronida
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jag.v11i1.18804

Abstract

Aglaonema ‘Siam Aurora’ is a foliage ornamental plant cultivar commonly used indoors, whose leaf coloration is influenced by shade levels. This study aimed to determine the effect of shade intensity on the vegetaitif and morphological changes of Aglaonema ‘Siam Aurora’. The experiment was arranged in a Randomized Complete Block Design (RCBD) with four treatments: control (no shade), and shading using 55% paranet in one, two, and three layers. Observations were made on changes in leaf and stem color using the RHS Colour Chart, as well as on plant morphological growth. The results showed that shading had no significant effect on plant height, leaf length, and leaf width, but significantly affected the number of leaves during weeks 6 to 8. The color of the center and edges of the leaves developed optimally under all treatments, indicating the plant's physiological adaptation to low light conditions. Stem color differed in the early stages but tended to become uniform by week 8. Leaf shape showed initial variation but became uniform after the fifth week. These findings suggest that Aglaonema ‘Siam Aurora’ has high tolerance to low to moderate light intensity, and shading with one to two layers of 55% paranet is effective in maintaining leaf morphology and color. More extreme shading levels and longer planting durations are recommended to further explore morphological responses.
Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro Nofitria, Atika Sari; Violita, Violita; Novita, Linda; Hindaningrum, Irni Furnawanthi; Elya, Mardoni; Kartiman, Roni
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6885

Abstract

Pterocarpus indicus Willd. also known as redwood is a native Philippine species belonging to the Fabaceae family that has many uses and high economic value and highly exploited. However, it is not accompanied by good regeneration which causes this species to be categorized as endangered (IUCN v. 2021-1). The in vitro technique is an alternative to overcome this problem. This study aims to determine the response of P. indicus Willd. shoot growth to the addition of BAP and TDZ in vitro. The research was conducted from August to December 2023, at the Biotechnology Laboratory, KST BJ. Habibie Puspiptek Serpong, South Tangerang, Banten. This study used a completely randomized design (CRD) consisting of 9 treatments and 14 replications. The treatments consisted of: R0 = Control, R1 = BAP 0,25 ppm, R2 = BAP 0,5 ppm, R3 = BAP 0,75 ppm, R4 = BAP 1 ppm, R5 = TDZ 0,25 ppm, R6 = TDZ 0,5 ppm, R7 = TDZ 0,75 ppm, R8 = TDZ 1 ppm. The results showed that the concentration of BAP 0,5 ppm gave the best results in height of shoot and TDZ 0,25 ppm gave optimum results in the number of shoots compared to the control and other treatment concentrations.
Co-Authors Agus Purwito Budiarni, Kasandra Dewi Sukma Elya, Mardoni Hardiani, Lany Irni Furnawanthi Hindaningrum Kubil Linda Novita Manalu, Doni Sahat Tua Marithasari, Harries Muharromah, Restu Siwi Nofitria, Atika Sari Rusmiyati, Henny Syarifah Iis Aisyah Tirtana, Arif Ulil Azmi Nurlaili Afifah Violita Violita