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DESCRIPTION OF ACID-RESISTANT BACTERIA EXAMINATION IN PATIENT FOLLOW UP TUBERCULOSIS IN THE WORKING AREA OF BLUD PUSKESMAS PERUMNAS, LUBUKLINGGAU BARAT I Widiawati Widiawati; Witi Karwiti; Ardiya Garini; Sri Hartini Harianja
Journal of Medical Laboratory and Science Vol 1 No 1 (2021): JMLS : Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.997 KB) | DOI: 10.36086/medlabscience.v1i1.656

Abstract

Background: Tuberculosis (TB) is an infectious disease caused by stem-shaped bacteria known as Mycobacterium tuberculosis. Transmission through saliva media or phlegm sufferers containing pulmonary tuberculosis basil. Pulmonary TB is a major public health problem with the number 3rd most in the world after China and India, with about 10% of the total number of people with pulmonary TB in the world. There are an estimated 539,000 new cases and 101,000 deaths each year. The number of pulmonary TB events in Indonesia characterized by the presence of positive acid-resistant bacteria in patients is 110 per 100,000 inhabitants. The purpose of the study to find out the description of the examination of acid-resistant bacteria in people with Pulmonary TB in the working area of BLUD Puskesmas Perumnas Lubuklinggau Barat Lubuklinggau District in 2018. Method: This study is descriptive research with cross sectional study design with axial. sampling method. Result: After conducting research on lung TB follow-up patients as many as 22 people who were originally positive for Pulmonary TB in the working area of BLUD Puskesmas District Perumnas Lubuklinggau Barat city I in 2018 obtained the results of research as many as 22 people (100 %) BTA test results were negative, based on education obtained by 9 people (40.9%) universities with negative BTA results and 13 people (59.1%) low education negative BTA results. Based on knowledge obtained by 22 people (100%) have good knowledge with negative BTA results. Based on the compliance of taking drugs obtained 22 people (100%) have adherence to ingesting the drug in accordance with negative BTA results. Based on the attitude of health workers obtained 22 people (100%) stated the attitude of health workers have a good attitude. Conclusion: BTA examination in all TB patients followed up with negative results.
SERA POOLED STABILITY AS A SGPT CONTROL MATERIAL WITH STORAGE TIME AND TEMPERATURE VARIATION Wuni Sri Lestari; Witi Karwiti; Aminahtun Latifah; Yenni Listiani; Sri Hartini Harianja
Journal of Medical Laboratory and Science Vol 2 No 1 (2022): JMLS: Journal of Medical Laboratory and Science
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.474 KB) | DOI: 10.36086/medlabscience.v2i1.1255

Abstract

To improve the quality of laboratory examination results, it is absolutely necessary to carry out quality assurance and these activities usually require control materials. The price of expensive commercial control materials is an obstacle to carrying out laboratory quality control. Efforts to maintain quality control are using pooled sera as control material. One of the examinations that is often done is the examination of SGPT (Serum Glutamic Pyruvate Transaminase) blood. The choice of SGPT parameter is expected to represent the type of enzyme assay. The purpose of this study was to determine the stability of pooled sera used as control material for SGPT examination with time and temperature variations. Methods: This research is an experimental study, which was carried out using a collection of serum found in the TLM Clinical Chemistry Laboratory of the Health Ministry of Health, Jambi. By using 2 treatments, the samples were stored at a temperature of 20-25oC and -5oC. Data analysis using ANOVA and coefficient of variation value. Results: The results showed a significant value (p-value) of SGPT of p = 0.008. A significant value (p-value) which is greater than the value of = 0.05, indicates that there is an effect of storage time and temperature variations. Conclusion : There is a significant effect of storage time and temperature variation of pooled sera control material on the stability of SGPT examination.