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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production
Wirdateti Wirdateti, Wirdateti
Pusat Penelitian Biologi LIPI, Jl. Raya Jakarta-Bogor, Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary Other

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Keragaman Genetik Rusa Sambar (Rusa unicolor), Pemanfaatan dan Implikasinya Untuk Konservasi Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.385 KB) | DOI: 10.14203/jbi.v8i1.3071

Abstract

Genetic Diversity of Sambar Deer (Rusa unicolor) in Utilization and Implications for Conservation. The deer sambar (Rusa unicolor) is one of species of Genus Rusa, was widely distributed in South Asia antil South East Asia. This species in Indonesia was widely distributed in Kalimantan and Sumatra island, but now, the hunting, poaching and habitat loss have reduced its populations drasstically. In order to provide useful information for its conservations. The genetic diversity and population structure of the wild sambar deer was observed by analyzing the 962 bp long of fragment mitochondrial DNA (mtDNA) control region (D-loop)fragment. The result detected, twenty different haplotypes from 22 samples from Borneo and Sumatra. Overall, sambar deer have a relatively high genetic diversity compared to other the ceervid species, with a haplotype diversity (h) 0.9870 and nucleotide diversity (ð) 2.931±0.260%.The genetic distance of Borneo populations (East Borneo) higher compared with Sumatra’s populations that is d = 0.018±0.003 on Borneo and d = 0.009±0.002 on Sumatra. The structure of phylogenetic tree showed that two populations were separate based on the haplotypes differences.Keywords: conservation, control region, genetic diversity, sambar deer.
Analisis Sekuen DNA Mitokondria Cytochrome Oxidase I (COI) mtDNA Pada Kukang Indonesia (Nycticebus spp) sebagai Penanda Guna Pengembangan Identifikasi Spesies Wirdateti, Wirdateti; Indriana, Eka; Handayani, Handayani
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1180.665 KB) | DOI: 10.14203/jbi.v12i1.2322

Abstract

Slow lories is small size primates of the genus Nycticebus that are in endangered status. Habitat pressure andhigh trade as pet animals has made the population decline both quantitatively and qualitatively, therefore it isnecessary to save the slow lories through conservation action. Morphologically, the individuals are difficult tobe distinguished among the species, therefore making much harder in identification for conservation purposessuch as from confiscated individuals. Therefore molecular marker is needed through mitochondrial DNA usingCOI gene sequences analysis is needed for identification. This study used 24 samples of slow lories consistedof 2 from Kalimantan, 9 from Java and 13 from Sumatra. The result indicated that in the 660 bp there were 62variable sites with 15 haplotypes: 4 haplotypes of N. javanicus, 9 haplotypes N. coucang, and 2 haplotypes N.menagensis. Base on nucleotide variations at specific sites, it was formed haplotype differences among species.The average genetic distance between species showed that Kalimanatan population were closer to thepopulation of Sumatra (d= 0.042 ± 0.006) compared with Java population (d= 0.059 ± 0.009), whereas thegenetic distance within population showed the population of Java had low diversity (d= 0.002) with a meannucleotide differences 2.6. Thus Jawan slow loris (N. javanicus) should be conserved.Keywords: Nycticebus spp., molecular, COI, conservation, marker
Pakan Alami dan Habitat Kukang (Nycticebus coucang) dan . Tarsius (Tarsius bancanus) Di Kawasan Hutan Pasir Panjang, Kalimantan Tengah Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.21 KB) | DOI: 10.14203/jbi.v3i9.3279

Abstract

ABSTRACTFeeds and habitat utilization of Slow Loris (Nycicebus coucang) and Tarsier (Tarsiusbnncartus) in Pasir Panjang Forest, Central Kalimantan. This research was conducted inthe production forest of Saka and Padang Babuluh in Pangkalan Bun. Study sites werelocated at 9-42 m above sea level. Data being collected were the type of feed, nest sites andhabitat utilization. During the observation, 44 plants species from 17 families were identifiedbeing consumed and nest site by the slow lories. Twenty one species from 17 families wereidentified being habitat by the tarsier. Nest were built on growing trees and litters were usedas bedding.Keywords : Nycticebus coucang, Tarsius bancanus, feeds, habitat, Pasir Panjang Forest
Keragaman Genetik pada Kukang (Nycticebus coucang) Berdasarkan pada gen 12S rRNA Mitokondria Wirdateti, Wirdateti; Okayama, Toshinao; Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3478

Abstract

ABSTRACTGenetic Diversity of Slow loris (Nycticebus coucang) based on Mitochondrial 12S rRNA gene. The research on genetic diversity of slow loris Nycticebus coucang (kukang) was carried out. The samples are 12 individuals from three locations (Sumedang and Jember in Java, and Lampung in Sumatera). Total DNA was extracted from blood and tissue. The mitochondrial 12S rRNA nucleotide sequences were determined to investigate genetic diversity of this species. This region was amplified by using L1091 and H 1478 primers by PCR. As a result of the analysis for 386 bp nucleotide sequence, five haplotypes were found, two from Java and three from Sumatra, respectively.Key words : Slow lories, Nycticebus coucang, genetic diversity, 12S rRNA mtDNA
Konsumsi dan Efisiensi Pakan pada Kukang (Nycticebus coucang) di Penangkaran Wirdateti, Wirdateti; Puspitasari, Dewi; Diapari, Didid; Tjakradidjaja, Anita S
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.63 KB) | DOI: 10.14203/jbi.v3i3.3474

Abstract

ABSTRACTFeed Intake and Efficiency of Slow Loris (Nycticebus coucang) in Captivity. Study on feed intake and efficiency of captive slow loris (Nycticebus coucang) has been conducted at the Division of Zoolgy, Research Center for Biology – LIPI for five weeks. Three individuals were placed in three cages, with its sizes of 1.2 x 1.7 x 1.9 m (one cage) and 0.8 x 1.7 x 1.9 m (two cages). Feeds consisting of papaya, banana, coconut, passion fruit, guava, sweet corn, quail egg, and white bread, were given ad libitum. Average body weight of slow loris in the beginning of the study was 587 gram/head and at the end was 648.33 gram/head. Average of feed consumption was 317.26 gram/kgBW/day. Average of feed efficiency and protein efficiency ratio (PER) was 0.0270 and 0.0945, respectively.Key words: Feed, slow loris, Nycticebus coucang, captivity, feed efficiency, PER.
KONSUMSI DAN EFISIENSI PAKAN PADA KUKANG (NYCTICEBUS COUCANG) DI PENANGKARAN Wirdateti, Wirdateti; Puspitasari, Dewi; Diapari, Didid; Tjakradidjaja, Anita S
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3474

Abstract

ABSTRACTFeed Intake and Efficiency of Slow Loris (Nycticebus coucang) in Captivity. Study on feed intake and efficiency of captive slow loris (Nycticebus coucang) has been conducted at the Division of Zoolgy, Research Center for Biology ? LIPI for five weeks. Three individuals were placed in three cages, with its sizes of 1.2 x 1.7 x 1.9 m (one cage) and 0.8 x 1.7 x 1.9 m (two cages). Feeds consisting of papaya, banana, coconut, passion fruit, guava, sweet corn, quail egg, and white bread, were given ad libitum. Average body weight of slow loris in the beginning of the study was 587 gram/head and at the end was 648.33 gram/head. Average of feed consumption was 317.26 gram/kgBW/day. Average of feed efficiency and protein efficiency ratio (PER) was 0.0270 and 0.0945, respectively.Key words: Feed, slow loris, Nycticebus coucang, captivity, feed efficiency, PER.
KERAGAMAN GENETIK RUSA SAMBAR (RUSA UNICOLOR), PEMANFAATAN DAN IMPLIKASINYA UNTUK KONSERVASI Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3071

Abstract

Genetic Diversity of Sambar Deer (Rusa unicolor) in Utilization and Implications for Conservation. The deer sambar (Rusa unicolor) is one of species of Genus Rusa, was widely distributed in South Asia antil South East Asia. This species in Indonesia was widely distributed in Kalimantan and Sumatra island, but now, the hunting, poaching and habitat loss have reduced its populations drasstically. In order to provide useful information for its conservations. The genetic diversity and population structure of the wild sambar deer was observed by analyzing the 962 bp long of fragment mitochondrial DNA (mtDNA) control region (D-loop)fragment. The result detected, twenty different haplotypes from 22 samples from Borneo and Sumatra. Overall, sambar deer have a relatively high genetic diversity compared to other the ceervid species, with a haplotype diversity (h) 0.9870 and nucleotide diversity (ð) 2.931±0.260%.The genetic distance of Borneo populations (East Borneo) higher compared with Sumatra?s populations that is d = 0.018±0.003 on Borneo and d = 0.009±0.002 on Sumatra. The structure of phylogenetic tree showed that two populations were separate based on the haplotypes differences.Keywords: conservation, control region, genetic diversity, sambar deer.
ANALISIS SEKUEN DNA MITOKONDRIA CYTOCHROME OXIDASE I (COI) MTDNA PADA KUKANG INDONESIA (NYCTICEBUS SPP) SEBAGAI PENANDA GUNA PENGEMBANGAN IDENTIFIKASI SPESIES Wirdateti, Wirdateti; Indriana, Eka; Handayani, Handayani
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2322

Abstract

Slow lories is small size primates of the genus Nycticebus that are in endangered status. Habitat pressure andhigh trade as pet animals has made the population decline both quantitatively and qualitatively, therefore it isnecessary to save the slow lories through conservation action. Morphologically, the individuals are difficult tobe distinguished among the species, therefore making much harder in identification for conservation purposessuch as from confiscated individuals. Therefore molecular marker is needed through mitochondrial DNA usingCOI gene sequences analysis is needed for identification. This study used 24 samples of slow lories consistedof 2 from Kalimantan, 9 from Java and 13 from Sumatra. The result indicated that in the 660 bp there were 62variable sites with 15 haplotypes: 4 haplotypes of N. javanicus, 9 haplotypes N. coucang, and 2 haplotypes N.menagensis. Base on nucleotide variations at specific sites, it was formed haplotype differences among species.The average genetic distance between species showed that Kalimanatan population were closer to thepopulation of Sumatra (d= 0.042 ± 0.006) compared with Java population (d= 0.059 ± 0.009), whereas thegenetic distance within population showed the population of Java had low diversity (d= 0.002) with a meannucleotide differences 2.6. Thus Jawan slow loris (N. javanicus) should be conserved.Keywords: Nycticebus spp., molecular, COI, conservation, marker
PAKAN ALAMI DAN HABITAT KUKANG (NYCTICEBUS COUCANG) DAN . TARSIUS (TARSIUS BANCANUS) DI KAWASAN HUTAN PASIR PANJANG, KALIMANTAN TENGAH Wirdateti, Wirdateti
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i9.3279

Abstract

ABSTRACTFeeds and habitat utilization of Slow Loris (Nycicebus coucang) and Tarsier (Tarsiusbnncartus) in Pasir Panjang Forest, Central Kalimantan. This research was conducted inthe production forest of Saka and Padang Babuluh in Pangkalan Bun. Study sites werelocated at 9-42 m above sea level. Data being collected were the type of feed, nest sites andhabitat utilization. During the observation, 44 plants species from 17 families were identifiedbeing consumed and nest site by the slow lories. Twenty one species from 17 families wereidentified being habitat by the tarsier. Nest were built on growing trees and litters were usedas bedding.Keywords : Nycticebus coucang, Tarsius bancanus, feeds, habitat, Pasir Panjang Forest
KERAGAMAN GENETIK PADA KUKANG (NYCTICEBUS COUCANG) BERDASARKAN PADA GEN 12S RRNA MITOKONDRIA Wirdateti, Wirdateti; Okayama, Toshinao; Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3478

Abstract

ABSTRACTGenetic Diversity of Slow loris (Nycticebus coucang) based on Mitochondrial 12S rRNA gene. The research on genetic diversity of slow loris Nycticebus coucang (kukang) was carried out. The samples are 12 individuals from three locations (Sumedang and Jember in Java, and Lampung in Sumatera). Total DNA was extracted from blood and tissue. The mitochondrial 12S rRNA nucleotide sequences were determined to investigate genetic diversity of this species. This region was amplified by using L1091 and H 1478 primers by PCR. As a result of the analysis for 386 bp nucleotide sequence, five haplotypes were found, two from Java and three from Sumatra, respectively.Key words : Slow lories, Nycticebus coucang, genetic diversity, 12S rRNA mtDNA
Co-Authors A Kundarmasno, A ALEX SUMADIJAYA Bram Brahmantyo, Bram Dewi Puspitasari Didid Diapari G Semiadi GONO SEMIADI Hadi Dahrudin, Hadi Hellen Kurniati, Hellen Indriana, Eka Indriana, Eka Jamhari Jamhari M Mansur, M Ni Luh Phadmacanty, Ni Luh Nugraha, R Taufiq P Okayama, Toshinao Okayama, Toshinao Reksodihardjo, Andi Riyanto, Awal Soemarno, Suprayogo Tjakradidjaja, Anita S Tjakradidjaja, Anita S Wartika Rosa Farida Widyastuti, SK Yulianto Yulianto