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All Journal Jurnal Sumberdaya Hayati 3BIO: Journal of Biological Science, Technology and Management Current Research on Biosciences and Biotechnology
Lili Melani
School Of Life Sciences And Technology, Institut Teknologi Bandung, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Effective removal of chromium from contaminated water using horizontal subsurface flow constructed wetland with Napier grass (Pennisetum purpureum) Taufik Taufikurahman; Lili Melani; Natasha Lumongga Hugo Sianturi; Ni Komang Devi Amrita Wirawan; Aisyah Fairuz Saifullah
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/226W5GYH

Abstract

This study aims to explore the potential of using Napier grass (Pennisetum purpureum) as a low-cost alternative for treating wastewater contaminated with chromium (Cr) in the tannery industry. Traditional chemical treatment processes are costly, leading to many industries not committing to treatment. Phytoremediation, the use of plants to remove contaminants from the environment, could be a viable solution. The study conducted an experiment to test the tolerance and effectiveness of P. purpureum in treating wastewater containing Cr(VI) at concentrations of 20 ppm, 40 ppm, and tannery wastewater. The plant was grown in a constructed wetland pond using a batch system for 31 days. The results indicate that P. purpureum can survive and grow well in all treatments, showing tolerance to Cr. The plant's phytoremediation capacity was effective at 20 ppm of Cr(VI) as demonstrated by the chemical and physical characteristics of the medium (BOD, COD, pH, and TDS). The plant was able to remove a significant amount of total Cr and Cr(VI), with removal capacity ranging from 71.79% to 99.96%. The translocation factor (TF) for Cr in the roots was less than one, indicating that the plant has the potential to be used as a phytoremediator for Cr by accumulating it in its roots.
The influence of chitosan on plant growth and β-sitosterol content in adventitious roots of Talinum paniculatum in NFT hydroponic cultivation system Widodo, Sinta Oktaviani Wahyu; Faizal, Ahmad; Melani, Lili
Current Research on Biosciences and Biotechnology Vol. 5 No. 2 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.5.2/JH7W60YM

Abstract

β-sitosterol is the most abundant type of phytosterol found in nature and it offers numerous health benefits for humans including anticancer, antidiabetic, and blood cholesterol-lowering properties. One biomass with a significant β-sitosterol content is the Javanese ginseng root (Talinum paniculatum). However, Javanese ginseng has not been fully exploited and is often perceived as a mere wild plant. This research was conducted by cultivating Javanese ginseng using the Nutrient Film Technique (NFT) hydroponic method along with the addition of chitosan elicitors at various concentrations: 0, 12.5, 25, and 50 ppm. The purpose of this research was to determine the optimal chitosan concentration for the growth of Javanese ginseng, the β-sitosterol content, and the productivity of β-sitosterol in Javanese ginseng roots cultivated using the NFT hydroponic method. The research revealed that a 12.5 ppm chitosan concentration could increase the root weight by 8.785 g and the shoot weight by 88 g. The growth rate increased to 3.457 g/day and the productivity of β-sitosterol in the roots reached 25.084 mg/m2/month. On the other hand, the addition of chitosan led to a decrease in the root-to-shoot ratio, indicating an improved cultivation environment compared to cultivation without chitosan. Furthermore, the results of the research also indicated that the addition of chitosan did not affect the β-sitosterol content in the Javanese ginseng roots. Therefore, it can be concluded that the optimal chitosan concentration that positively impacts Javanese ginseng cultivated using the NFT hydroponic method is 12.5 ppm.
Analisis Kadar Protein dan Pertumbuhan Biomassa dari Chlorella sp. dan Nannochloropsis sp. pada Kultur Tunggal dan Campuran yang Diperoleh dari Remediasi Anaerobically Digested Dairy Manure Wastewater  (ADDMW) Menggunakan Bioreaktor Open Raceway Pond: Biomass and Protein Production of Chlorella spp. and Nannochloropsis spp. in Single and Mixed Cultures on Anaerobically Digested Dairy Wastewater Muhammad Farhan Aidira; Taufikurahman; Lili Melani
Jurnal Sumberdaya Hayati Vol. 12 No. 2 (2026): 2026
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.12.2.71-78

Abstract

Microalgae are known for their ability to produce protein and reduce certain pollutant levels. One of the main challenges in microalgae cultivation is the provision of suitable synthetic growth media. Anaerobically Digested Dairy Manure Wastewater (ADDMW) has potential as an alternative growth medium due to its relatively high nutrient content. This study aims to analyze the effect of ADDMW-based cultivation on the growth rate, biomass production, and protein production of Chlorella spp. and Nannochloropsis spp., cultivated both in monoculture and mixed culture systems using an open raceway pond bioreactor. Cultivation was carried out using Chlorella spp. and Nannochloropsis spp. with an initial volume ratio of 1:1 (v/v) over a 7-day period, and the ADDMW wastewater was diluted 25 times prior to use. The highest specific growth rate, biomass yield, and biomass productivity were observed in the mixed culture, with values of 1.47 day⁻¹, 0.95 g·L⁻¹, and 0.28 g·L⁻¹ day⁻¹, respectively. The highest protein content was also obtained from the mixed culture, reaching 56.25% of dry biomass, whereas the protein content in monocultures of Chlorella spp. and Nannochloropsis spp. was 40.42% and 40.11%, respectively.  
Value Chain and Techno-Economic Analysis of Biodiesel Production from Rubber Seeds in Banyuasin, Indonesia Adela Damika Putri; Muhamad Aldi Nurdiansyah; Lili Melani; Angga Dwiartama; Muhammad Yusuf Abduh
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 2 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.2.3

Abstract

Banyuasin Regency, located in the province of South Sumatra, has the largest area of rubber plantations in Indonesia, with huge potential as biodiesel feedstock, and generates income for rubber planters through the exploitation of the seeds. This study focuses on the value chain of the exploitation of seeds for biodiesel and its economic benefits to the planters using the Hayami method. The snowball and stratified random sampling methods were used to determine the pricing of the seeds. The value chain actors include the rubber planters, the crumb rubber processing and marketing unit, the biodiesel processing unit, and the consumers, which operate through the market governance structure. The findings indicate that rubber seed processing generates an added value of IDR 4,937/kg, with a value-added ratio of 64%, classifying it as a high-value commodity. Furthermore, integrating rubber seed value chain activities has the potential to increase farmers’ annual income by IDR 3,850,000, contributing to financial sustainability. A techno-economic analysis confirms the feasibility of intermediate-scale biodiesel production in Banyuasin Regency, with a benefit-cost ratio of 1.35, demonstrating its potential to create substantial economic value from rubber seeds. These findings highlight a strategic opportunity for the crumb rubber processing and marketing unit in the Banyuasin Regency to enhance its role within the supply chain while improving farmer profitability. By optimizing value chain efficiency and ensuring market stability, rubber seed-based biodiesel production can contribute to economic growth, rural development, and renewable energy advancement in Indonesia.   
Co-Authors Abduh, Muhammad Yusuf Adela Damika Putri Ahmad Faizal Aisyah Fairuz Saifullah Dwiartama, Angga Muhamad Aldi Nurdiansyah Muhammad Farhan Aidira Natasha Lumongga Hugo Sianturi Ni Komang Devi Amrita Wirawan Taufik Taufikurahman Taufikurahman Widodo, Sinta Oktaviani Wahyu