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All Journal Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan
Sulistyo Dwi Kartining Putro, Sulistyo Dwi Kartining
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Biochemistry, Genetics & Molecular Biology Education Environmental Science

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Perkembangan Konsentrasi Hormon Pertumbuhan untuk Metamorfosis Ulat Sutera (Bombyx Mori L.) Putro, Sulistyo Dwi Kartining; Lestari, Umie; Lukiati, Betty
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Peranan hormon dalam metamorfosis meliputi proses pengelupasan kulit larva, dan pembentukan pupa pada serangga holometabola. Hormon Juvenil menjadi salah satu kunci hormon yang berperan dalam mengatur aktivitas pertumbuhan serangga.Tujuan dari penelitian ini adalah untuk melakukan isolasi 10kDa yang merupakan hormon juvenil sebagai pengatur utama hormon pertumbuhan dan prekursor utama diapause hormon.Penelitian ini merupakan penelitian eksploratif. Materi hormon 10kDa diisolasi dari hemolympha ulat sutera (Bombyx mori L.) fase Instar 3, instar 4, instar 5, serta fase pupa menggunakan phosphate buffered saline kemudian di running menggunakan SDS-PAGE dan dikoleksi dengan elektroelusi. Karkterisasi hormon 10 kDa pada instar 3, 4, 5 dan pupa meliputi penentuan berat molekul melalui SDS-PAGE dan kandungan protein dengan metode nanodrop spectrophotometer.Hasil running isolat hormon menggunakan metode SDS-PAGE diperoleh pita protein hormon yang terdiri atas 5 pita dengan berat molekul 62,42; 39,10; 30,94; 15,34; dan 10,3 kDa. Kadar Isolat protein hormon 10 kDa diperoleh dengan purifikasi menggunakan metode elektroelusi. Kandungan protein dalam isolate protein hormon 10 kDa pada instar 3 sebesar sebesar 0,121 mg/ml, instar 4 sebesar 0,012 mg/ml, dan instar 5 sebesar 0,117 mg/ml.
Pengembangan Buku Ajar Perkembangan Hewan Berbasis Penelitian Metamorfosis Ulat Sutera Bombyx Mori L. Putro, Sulistyo Dwi Kartining; Lestari, Umie; Lukiati, Betty
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol. 1, No. 7
Publisher : citeus

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Abstract

The developed teaching material in this study is in the form of a research-based textbook on silkworm Bombyx mori L hormone. It was aimed to develop the textbook on animal development course that will be used as one of supplementary learning textbook for students of S1 Biology Department of State University of Jember. The development of this textbook deployed the Hannafin and Peck developmental model which consists of three phases: (1) Needs Assessment (Analysis Purposes Phase), (2) Design (Design Phase), and (3) Developing/ Implementing (Development and Implementation Phases). It is a developmental model of instructional design which is oriented to products. The data were obtained starting from the needs analysis, product design, product manufacturing, and product validation. The instrument used to collect the research data development used a questionnaire and interview guidelines. These data were analyzed descriptively. The results of the analysis showed that this textbook has met the eligibility requirements. The feasibility of a limited test of individual students was 94.7% which was obtained with a valid category, the test feasibility of media experts obtained by 93.35% with a valid category, and the test feasibility of material presentation of 98.89% with a valid category and appropriate to be used.
Co-Authors Betty Lukiati Umie Lestari