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Compatibility Test Consorcium of Thermophilic Bacteria Producing Xylanase Enzym from The Hot Water of Mudiak Sapan (MS18, MSS15, MSS11, MS16) Irdawati; Indrawani Matondang; Linda Advinda; Azwir Anhar; Yusrizal Y
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4761

Abstract

Mudiak Sapan hot spring, South Solok Regency, West Sumatra is one of the hot springs where there are various species of thermophilic bacteria that produce xylanase enzymes. The xylanase enzyme is a group of enzymes that has the ability to hydrolyze xylan (hemicellulose) or polymers into xylose and xylo-oligosaccharides. The purpose of this study was to test the compatibility of bacterial isolates to become a xylanase-producing bacterial consortium. This research is a descriptive research that informs the compatibility of thermophilic bacterial isolates from the Mudiak Sapan hot springs. The method used in this study was disk diffusion, based on the research, it was found that 5 combinations of compatible xylanase enzyme-producing isolates were MS18 x MSS11; MS18 x MSS15; MSS18 x MS16 ; MSS11 x MS16 ; MSS15 x MS16 and 1 combination of incompatible isolates namely MSS11 x MSS15 which had an inhibition zone of 0.645 cm.
The Potential of Hot Water Mudiak Sapan Thermophilic Bactery Consortium Formulation in Producing Xylanase Enzyme Irdawati; Indrawani Matondang; Linda Advinda; Azwir Anhar; Yusrizal Y
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5309

Abstract

The xylanase enzyme has high commercial value in the industrial sector, including the food industry, animal feed, bleaching of pulp/pulp, lignocellulosic bioconversion as fuel, and in the food industry, namely the cheese, bread and meat industries, while in the non-food industry this can be used in detergents. Xylanase enzymes can be produced from microorganisms, one of which is thermophilic bacteria. Thermophilic bacteria are known to be able to produce thermostable enzymes and proteins that are heat resistant. Xylanase production in compatible bacterial consortia was higher than monoculture. The purpose of this study was to determine the bicultur consortium that has the potential to produce xylanase enzymes. This research is an experimental research. Enzyme activity testing used the Miller method with DNS (Dinitrosalycilic acid) reagent using a completely randomized design (CRD) with 3 repetitions. Data on the results of xylanase activity were analyzed by ANOVA test and DMRT follow-up test with a 5% significance level.The results of the thermophilic bacterial isolates in the consortium that have the potential to produce xylanase enzymes are MS18 & MSS15,  MSS15, MS16 and MS18 & MS16.