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Irdawati Irdawati

Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang

Author-ID : 2543053

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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UJI BAKTERIOLOGIS SUSU KEDELAI PRODUK RUMAH TANGGA YANG DI JUAL DIPASARAN Helpida, Helpida; Indriati, Gustina; Irdawati, Irdawati
Pendidikan Biologi Vol 2, No 2 (2013): Jurnal Mahasiswa Pendidikan Biologi Genap 2013-2014
Publisher : STKIP PGRI Sumbar

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ABSTRACKSoy milk is a food that has ingredients such as soy. Soy milk has a complete nutrient such as protein, carbohydrates, fats, minerals and vitamins. Complete nutrient content and high nutritional value, in convenient contaminated milk caused by microorganisms such as E. coli and Coliform. Associated with that we conducted this study aimed to determine the water quality of the bacteriological soy milk. This study was conducted from July to September 2013 is housed in the Laboratory of Microbiology Department of Biology, State University of Padang. This research is a descriptive study with random sampling. This study uses the Most Probable Number (MPN) using a two-stage trial testing the prediction (Presumtive Test) and test of certainty (Confirmative Test) with a range of 5:1:1. The results showed that soy milk is only 40% that meet the standards and 60% did not meet the standards set by the Ministry of Health No. 416/MENKES/PER/IX/1990 of the Terms and Drinking Water Quality Monitoring.

UJI DAYA HAMBAT EKSTRAK DAUN BUNGA TANJUNG (Mimusops elengi L.) TERHADAP PERTUMBUHAN Candida albicans Antika, Wella; Indriati, Gustina; Irdawati, Irdawati
Pendidikan Biologi Vol 1, No 1 (2014)
Publisher : STKIP PGRI Sumbar

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ABSTRACTÂ Â Petal cape ( Mimusops elengi L.) is one of the medicinal plants . Petal cape contains chemical compounds that act as anti- microbial . These chemical compounds are saponins , alkaloids and tannins , but it is not certain the extent of inhibition of petal cape on the growth of Candida albicans . Based on the research that has been done Inhibition Test Leaf Extract Flower Power Â Cape Â ( Â M. Â elengi Â L.) Â on Â growth Â ofÂ Â Â C. Â albicans Â . Â This Â researchÂ used Â Completely Randomized Design ( CRD ) with 7 treatments and 3 replications starting from AlbothylÂ Â Â control( A ) 2 % , cape flower leaf extract ( B ) 5 % , ( C ) 10 % , ( D ) 20 % , ( E ) 30 % , ( F ) 40 % , ( G) 50 % . Data were analyted using Analysis Of Variance ( ANNOVA ) . Study aimed to determine the inhibition of leaf extracts of flowers cape ( M. Elengi L. ) on the growth of C. albicans , and to determine the most effective concentration of leaf extracts of flowers cape ( M. Â elengi L.) to the growth ( C. albicans ) performed in microbiology laboratories UNP . The results showed that the leaf extract of flower cape ( M. elengi L.) were able to inhibit the growth of Candida albicans . And found that the most effective concentration in treatment C ( 10 % ) is equal to 1.74 mm , and the concentration of the smallest on the control that is equal to 1.34 mm . From the research that has been carried out it was concluded that the extract from the leaves of flowers tanjung with a concentration of 5 % to 50 % can inhibit the growth of C. albicans , meaning headland petals can be used as an alternative to treat canker sores in the mouth .

UJI DAYA HAMBAT EKSTRAK MENIRAN (Phyllanthus niruri L.) TERHADAP PERTUMBUHAN Candida albicans. Pratiwi Yusni, Melsi; Indriati, Gustina; Irdawati, Irdawati
Pendidikan Biologi Vol 1, No 1 (2014)
Publisher : STKIP PGRI Sumbar

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ABSTRACÂ Meniran ( Phyllanthus. niruri L. ) or known to the sidukuang anak a potent plant can treat a variety of diseases . Meniran ( P. niruri L.) which contains antimicrobial flavonoid, saponin and tanin that can inhibit the growth of C. albicans . Has done research on Power Test Inhibitory Meniran Extract ( Phyllanthus niruri ) on growth of C. Â albicans . This study used a Completely Randomized Design ( CRD ) with 7 treatments and 3 replications , treatment begins albothyl 2 % of control ( A ), Concentration 5 % ( B ), concentration of 10 % ( C ), concentration of 30 % ( D ) , Concentration 40 % ( E ) , concentration of 50 % ( F ). Data processed by Analysis Of Variance ( ANNOVA ). This study aims to determine the inhibitory meniran extract ( P. niruri L.) on the growth of C. Â albicans , and to determine the most effective concentration of meniran ( P. niruri L.) on the growth of C. albicans has been done in the laboratory of the UNP . From the research that has been done , it can be concluded that the extract meniran ( P. niruri L.) ranging from 5 % concentration was able to inhibit the growth of C.Â Â albicans . The most effective concentration present in a concentration of 20 % .

DETEKSI KAPSUL DAN SLIME PADA BAKTERI PATOGEN YANG DIISOLASI DARI BENIH LELE DUMBO (Clarias gariepinus) Fadilah, Muhyiatul; Alberida, Heffi; Irdawati, Irdawati
Sainstek : Jurnal Sains dan Teknologi Vol 3, No 2 (2011)
Publisher : IAIN Batusangkar

Pathogenic bacterial ability to cause disease is called patoghenicity. Bacteria pathogenicity level stated as virulency. Bacterial virulency is determined by virulency agent, include certain structure of the cell and secrete produced into substrate. Capsul and slime are specific cell structure which act as virulency agent. Capsul and slime presence is able to prevent phagocytosis by host antibody. This study aimed to confirm presence of capsul and slime produced by pathogenic bacterial isolated from Clarias gariepinus. These pathogens are Alcaligenes sp., Bacillus sp., Micrococcus sp., Proteus sp., Pseudomonas sp., and Serratia sp. This is a descriptive study. Observation has been done to detect intense layer arround the cells by using negative staining methode. Result of study has informed that Alcaligenes sp., Bacillus sp., Micrococcus sp., Proteus sp., Pseudomonas sp., dan Serratia sp., examined do not form capsul and slime.Key words: Capsul and slime, bacterial pathogen, Clarias gariepinus

SPINACH (Amaranthus tricolor L.) GROWTH USING ORGANIC FERTILIZER FROM Trichoderma harzianum DECOMPOSITION RESULT Anhar, Azwir; Advinda, Linda; Mulyadi, Rival; Irdawati, Irdawati; Leilani, Irma
Sainstek : Jurnal Sains dan Teknologi Vol 9, No 2 (2017)
Publisher : IAIN Batusangkar

Long term period of using fertilizers is not beneficial for soil fertility due to decreasing soil organic matter content. Otherwise, the source of organic matter widely available as waste in the environment are not used optimally because the naturally decomposition quite long time. This problem can be overcome by utilizing various types of microbes, such as Trichoderma. This research conducted to determine the effect of organic fertilizer from the decomposition result of T. harzianum in enhancing growth of spinach plant. This study used a completely randomized design (CRD) with 6 treatments and 4 replications. The treatments were doses variation of decomposition of organic material; 0 g, 15 g, 30 g, 45 g, 60 g and 75 g. Data were analyzed with ANOVA and continued with Duncan New Multiple Range Test (DNMRT). The results showed that the application doses of organic fertilizer from decomposition result influences height, number of leaves, leaf area, fresh weight and dry weight of spinach plants. Optimal dose for all growth is 45 grams/polybag.

UJI BAKTERIOLOGIS AIR SUMUR PEMUKIMAN PENDUDUK DI SEKITAR TEMPAT PEMBUANGAN AKHIR SAMPAH Irdawati, Irdawati; Fifendy, Mades; Kurniati, Deni
Sainstek : Jurnal Sains dan Teknologi Vol 4, No 2 (2012)
Publisher : IAIN Batusangkar

The purpose of this study was to determine the quality of well water in residential areas around the landfill of trash (TPA), Balai Gadang Aia Dingin, Padang, viewed from bacteriological aspects. This study was conducted from March to April 2012 in the Laboratory of Microbiology Department of Biology, Science Faculty, Padang State University (UNP). Samples were taken using Proposive sampling method with 10 wells that are encountered by the first well of the landfill to the distance between 0-100 meters. This study uses Most Probable Number (MPN) using a two-stage testing is Presumtive Test and Confirmative Test with a combination of 3:3:3. can be concluded that the quality of well water residential areas around the landfill 90% did not meet the standards set by the Ministry of Health (KEPMENKES) No. RI. 492/MenKes/PER/IV/2010. Key words : TPA Aia Dingin Padang, bacterial aspects, quality of well water

NEED ANALYSIS OF INSTRUCTIONAL MEDIA WITH ESQ NUANCE AS SUPLEMENT IN SENIOR HIGH SCHOOL Khalif Alfajri; Ardi Ardi; Ganda Hijrah Selaras; Irdawati Irdawati; Heffi Alberida; Sasmeri Sasmeri
ATRIUM PENDIDIKAN BIOLOGI Vol 6, No 3 (2021): Atrium Pendidikan Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/apb.v6i3.11621

The 2013 curriculum encourages students to be more active and independent when getting information about learning materials. Therefore, teachers are required to be able to increase students' learning motivation, one of which is by developing learning media that can attract students' learning interest. The purpose of this study was to see the needs of students for learning media with ESQ nuances about bacteria as a supplement to biology teaching materials for class X science SMAN 2 Payakumbuh. This type of research is descriptive quantitative research. The method used to collect data was done through distributing questionnaires to class XII students and interviewing two teachers, namely a biology teacher and an Islamic religious education teacher. In addition, the daily test results obtained by students were also used as data in this study. The results of the study revealed that students had difficulty in understanding biology learning about bacterial material due to the difficulty of observing micro learning objects plus less supportive learning media where the images on the learning media were of very low quality and colorless. This results in a lack of student motivation in participating in the learning process. For that we need a learning media that is easy to understand and has high quality images so that it can be studied independently by students.

The Activity Xylanase Enzyme Thermophilic Bacteria SSA 2 in Starter Variation Elfah rahma; Irdawati Irdawati
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11853RF00

Xylanase is extracellular enzyme that hydrolyzes xylan into xylose and xylooligosaccharides. The bacteria that produce the xylanase enzyme, namely SSA 2 isolate from the Sapan River Aro Solok Selatan hot spring, has a temperature of 75ºC and a pH of 8 or alkaline. Thermophilic bacteria can produce xylanase enzymes under optimum environmental conditions. Environmental optimization can be done by optimizing media composition, dissolved oxygen content, aeration, agitation, starter concentration, pH and temperature. This study aims to determine the specific activity of the xylanse enzyme at the starter concentration. The starter concentrations used were 2%, 4%, 6%, 8% and 10%. The highest specific activity of the xylanse enzyme was at a starter concentration of 4% (0.916 U/mL) this indicates that a low starter concentration can lead to good microbial growth.

THE EFFECT OF TEMPERATURE ON THE ACTIVITIES OF AMOBIL XILANASE ENZYMES IN THE PAPER BIOBLEACHING PROCESS (PULP) Irdawati Irdawati; Fauzana Ahmad; Syamsuardi Syamsuardi; Agustien Agustien; Y Rilda
Serambi Biologi Vol 5, No 2 (2020): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/6023RF00

Abstrak. Enzim xilanase merupakan enzim extraseluler yang mampu menghidrolisis xilan menjadi xilosa. Isolat SSA 2 merupakan isolat dari bakteri termofilik hasil isolasi dari air panas sapan sungai aro, yang memiliki kemampuan menghasilkan enzim xilanase. penggunaan xilanase merupakan salah satu alternative mengurangi pemakain clorin dalam proses pemutihan pulp. xilanase diamobilisasi dengan menggunkan NA alginate. pulp difermentasi dengan suhu 50ºC, 55ºC, 60ºC, 65ºC, 70 ºC, 75 ºC, 80ºC, selama 6 jam, suhu optimum yang menghasilkan bilangan kappa terendah dan aktivitas enzim yang tertinggi pada suhu 60ºC.

Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate Nada Nafion; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Cases of bacterial resistance to antibiotics are discussed with serious problems in the world of health. New antimicrobial compounds are needed which are more effective in treating infectious diseases. Isolate B.J.T.A-6 is an endophytic bacteria from Andalas plants (Morus macroura Miq.) which is known to be able to produce antimicrobial active compounds. Antimicrobial compounds can be produced by growing them on fermented media. The purpose of this study was to optimize the medium fermentation of Andalas endophytic bacteria of B.J.T.A-6 isolates in producing antimicrobial compounds. While medium fermentation is Nutrient Broth (NB), Muller Hinton (MH), and Luria Bertani Broth (LB). Antimicrobial activity tests were carried out by means of diffusion. The parameters used were the diameter of the inhibition zone against Staphylococcus aureus around the disc paper. The profile of medium fermentation optimization was analyzed statistically. The results showed that B.J.T.A-6 isolates could inhibit the highest growth of S. aureus by using LB fermentation medium.

Co-Authors Abdul Razak Abdul Razak Abdul Razak Abror Abror Agustien Agustien Ahmad, Nurafni Fajarna Do Andrian, Richard Anggraeni, Novicka Putri Anthoni Agustien Apriansa, Apriansa Ardi Ardi Armiliandi, Rada Aryeni Fatwah, Monika Az-Zahra, Fauziah Azwir Anhar Bob Refianto Deni Kurniati Des M Des Desi Nurmayenti Dezi Handayani Dezi Handayani Dwi Hilda Putri Elfah rahma Elsa Sri Handayani Fahra Fahra Fahra Fahra Fahra, Fahra Fajri, Dilham Hidayatul FAJRINA, SUCI Faradilla Rattriana Fatiha, Fathma Dwi Fauzana Ahmad Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Francantika, Manja Ghiffari, Muhammad Gustina Indriati Heffi Alberida Helpida Helpida Ilsa Septia Putri Inayatul Fatia Indrawani Matondang Irma Leilani Jannah Koftiah Khalif Alfajri Kurnia Wulansari Larashinda, Mentari Linda Advinda Lufri Lufri Luthfi, Afdal Mades Fifendy Marissa Angelina Maulina, Adinda Rizky Mei Niarti Melsi Pratiwi Yusni Monhartini, Monhartini Moralita Chatri Muhamad Sholichin Muhamad Sholichin Muhammad Aulia Rahman Muhammad Deedat Ayasy Muhyiatul Fadilah Mulia Mulia Mulia Mulyadi, Rival Muskhir, Mukhlidi Nada Nafion Nia Ramadhanti Nisa Afifah Nissha, Dwi Khayrun Nur Shofiatun Nisa Nur Vaizi Nurfatihah Z, Zahara Putri Andriani Putri Ardila Sari Putri Rachma Auliya Putri Rahma Padilla Putri Sarinami Rahmi, Elva Refni Syahleli Afni Rizka Putri Alti Roza Yolanda Sasmeri Sasmeri Selaras, Ganda Hijrah Sri Wahyuni Suci Fajrina Suherman, Donny Summaiati, Titi Syahrastani, Syahrastani Syamsuardi Syamsuardi Syamsurizal S Tomi Apra Santosa Uthami, Feranis Nadia Vauzia Vauzia Vestimarta, Aldi Wahyuda Violeta Violita Violita Violita Violita Viona, Alda Wahyuda Vestimarta, Aldi Wardah Hayati, Wardah Wella Antika Weni Yulastri Windi Nopitasari Wirda Taufik Witri Nofita Safitri Wulansari, Kurnia Y Rilda Y Yusrizal Yetria Rilda Yuandry, Syafrina Yusrizal Y Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zulyusri, Zulyusri

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