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All Journal Jurnal Biologi Udayana Biotropika Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Panrita Abdi - Jurnal Pengabdian pada Masyarakat ABDINE Jurnal Pengabdian Masyarakat
Muhammad Fauzi Arif
Laboratory Of Biology, Department Of Biology, Faculty Of Mathematics And Natural Sciences, Mulawarman University, Samarinda 75119, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Immunology & microbiology Other

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Jenis-jenis Araceae di lantai hutan Air Terjun Berambai, Samarinda, Kalimantan Timur Linda Oktavianingsih; Nur Miati; Muhammad Fauzi Arif; Medi Hendra
Jurnal Biologi Udayana Vol 27 No 1 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i01.p03

Abstract

Araceae merupakan herba dengan berbagai macam manfaat etnobotani yg dikenali dengan bunganya yang berbentuk tongkol dan tumbuh subur di kawasan hutan tropis. Hutan Air Terjun Berambai merupakan salah satu kawasan hutan sekunder dataran rendah yang sangat baik untuk pertumbuhan dan perkembangan familia Araceae. Tujuan dari penelitian ini adalah untuk mengetahui jenis dan karakteristik tumbuhan familia Araceae yang hidup di kawasan lantai hutan Air Terjun Berambai, Samarinda, Kalimantan Timur. Penelitian ini dilakukan dengan metode eksplorasi yaitu dengan menjelajahi kawasan Hutan Air Terjun Berambai. Hasil pengamatan menunjukan terdapat delapan jenis Araceae yang yaitu Pichinia disticha S.Y. Wong & P.C. Boice, Rhaphidophora australasica F.M. Bailey, Alocasia princeps W. Bull., Schismatoglottis calyptrata (Roxb.) Zoll. & Moritzi, Colocasia esculenta (L.) Schott, Aridarum sp., Amorphophalus paeoniifolius (Dennst.) Nicolson, dan Philodendron crassinervium Lindl. dengan karakter sintesis berupa berbatang basah, bunga berbentuk tongkol, dan berseludang. Tumbuhan familia Araceae yang ditemukan di Hutan Air Terjun Berambai tumbuh secara epifit maupun terestrial. Penelitian ini menjadi landasan data inventarisasi tumbuhan famili Araceae yang ada di lantai hutan Air Terjun Berambai, Samarinda, Kalimantan Timur yang belum ada sebelumnya.
Comparison of Lysis Buffers for Saliva DNA Extraction using Modified Magnetic-Silica Nanoparticle Beads Muhammad Fauzi Arif; Ahadi Damar Prasetya; Indra Lesmana; Niken Satuti Nur Handayani
Biotropika: Journal of Tropical Biology Vol. 11 No. 1 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.01.01

Abstract

Our previous study revealed that modified MAGSi (magnetic-silica nanopartivel beads) had various sizes and shapes that affected the DNA extraction process. In this study, we combined our MAGSi with several kinds of lysis buffer for DNA extraction to analyze the result. Human saliva DNA was extracted using three kinds of MAGSi. Each of them was made differently. We used ATL buffer, Triton Buffer, and without buffer for cell lysis. The concentration and purity of the extracted DNA were analyzed using spectrophotometry. Extracted DNA also was used as template DNA for DNA amplification using beta globin gene. This study revealed that our MAGSi-1 provided the best result for DNA concentration and purity. This study also revealed that DNA extraction using lysis buffer produced less concentration yield and purity value but it was more consistent. The concentration and purity value were different but consistent. All the extraction can be used as DNA template for amplification of human beta globin gene. Further studies are still needed to determine the effectiveness and consistency of the lysis buffer and our MAGSi.
PENDAMPINGAN BUDIDAYA LARVA TENTARA LALAT HITAM (HERMETIA ILLUCENS) KOMUNITAS PETERNAK SAPI DI SANGATTA, KALIMANTAN TIMUR Ari Sanjaya; Didit Suprihanto; Wulan Sari; Rudianto Rudianto; Rudy Agung Nugroho; Retno Aryani; Hetty Manurung; Muhammad Fauzi Arif; Iyut Jaya Toimsar
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 8 No. 1 (2024): Jurnal Panrita Abdi - Januari 2024
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v8i1.23750

Abstract

Black Soldier Fly larvae commonly known as maggot (Hermetia illucens), are currently gaining momentum to convert organic waste. Using bioconversion technology of organic waste with maggot, will not only help in decomposing waste but also the results of cultivation can improve the economy of society. This community service aimed to provide assistance in maggot cultivation for the cattle breeder community, Sangatta City, East Kalimantan province, so that they are familiar with and able to cultivate maggot, especially for producing high protein feed ingredients. The present community service activity was attended by a group of cattle breeders from the Sangatta area, East Kalimantan, and accompanied by a community service team from Mulawarman University. The activity lasted for three months and the implementation was carried out using the method of material presentation, discussion, question and answer, and the practice of maggot cultivation. The activity was carried out at the house of the Sangatta cattle farmer, East Kalimantan. As a result of the activity, cattle breeders have been able to start maggot cultivation and provide an evaluation that the activity is very important because it adds new insight and understanding about maggot cultivation, especially for food security in the area around the capital of the archipelago, Indonesia.  ---  Larva lalat tentara hitam atau dikenal awam sebagai belatung atau maggot dengan nama ilmiah (Hermetia illucens), saat ini popular dibudidayakan untuk merombak limbah organik. Dengan adanya teknologi biokonversi limbah organik dengan maggot, maka tidak hanya membantu dalam merombak limbah namun juga hasil budidaya dapat meningkatkan perekonomian. Pengabdian kepada masyarakat kali ini bertujuan memberikan pendampingan budidaya maggot bagi komunitas peternak sapi Sangatta, Kalimantan Timur, agar mengenal dan mampu membudidayakan maggot dan menghasilkan larva yang mengandung bahan pakan berprotein tinggi. Pengabdian diikuti oleh kelompok peternak sapi dari wilayah Sangatta, Kalimantan Timur dan didampingi oleh tim pengabdi dari Universitas Mulawarman. Kegiatan berlangsung total selama 3 bulan dan pelaksanaan dilakukan dengan metode presentasi materi, diskusi, tanya jawab dan praktek budidaya maggot. Kegiatan dilaksanakan di rumah peternak sapi Sangatta, Kalimantan Timur. Hasil kegiatan, peternak sapi telah mampu memulai budidaya maggot dan memberikan evaluasi bahwa kegiatan sangat penting karena menambah wawasan dan pemahaman baru tentang budidaya maggot terutama untuk ketahanan pangan di wilayah sekitar Ibukota Negara Nusantara, Indonesia.
Plant Chromosome and Karyotype Analysis Training for High School/MA/Equivalent Teachers in Samarinda and its Surrounding Areas Oktavianingsih, Linda; Aryani, Retno; Hariani, Nova; Rosadi, Imam; Pratiwi, Anggita Endar; Arif, Muhammad Fauzi
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 3 (2024): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.88892

Abstract

Increasing biology teachers’ comprehension of the principles of genetics is attainable through the implementation of specialized training sessions focused on chromosome and karyotype analysis. In this training, 21 high school biology teachers from Samarinda and Nears participated in a structured training program encompassing three stages. In the initial phase, participants were provided with materials on the fundamental aspects of genetics. Subsequently, in the second phase, hands-on training was imparted, focusing on acquiring skills in plant chromosome preparation techniques. Lastly, the third stage concentrated on refining skills to construct chromosome karyotyping. Completing all stages was required for participants, followed by submitting evaluation forms for assessment by the committee. The evaluation outcomes revealed that 84.30% of participants regarded the presentation and assistance provided by principal lecturer and assistants as highly commendable. At the same time, the remaining respondents expressed satisfaction with the qualityand adequacy of the training. This comprehensive training effectively equipped participants with specialized chromosome and karyotype analysis skills, and the participants notably absorbed the training materials. The anticipated outcome of enhanced teacher understanding, specifically in the knowledge of inheritance and chromosome biology, was to stimulate heightened levels of student engagement and learning interest within the biology classroom.
Pelatihan Teknik Penggunaan, Pengenalan Mikroskop dan Pengamatan Sel bagi Guru-Guru dan Siswa/i di SMP Negeri 5 Samarinda Manurung, Hetty; Kusuma, Ratna; Susanto, Dwi; Samsurianto, Samsurianto; Fauzi Arif, Muhammad
ABDINE: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2025): ABDINE : Jurnal Pengabdian Masyarakat
Publisher : Sekolah Tinggi Teknologi Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52072/abdine.v5i1.1240

Abstract

Rendahnya kompetensi guru dalam menggunakan mikroskop, pembuatan preparat dan pengamatan sel akibat keterbatasan fasilitas dalam pelaksanaan praktikum IPA, menjadi kendala di sebagian sekolah di Samarinda, termasuk SMP Negeri 5 Samarinda. Tujuan pengabdian masyarakat ini dilaksanakan adalah untuk meningkatkan kompetensi guru IPA dan siswa dalam penggunaan mikroskop, pembuatan preparat, dan pengamatan sel. Kegiatan dilaksanakan dengan metode pelatihan (training) diikuti oleh 45 peserta yang terdiri dari 8 guru IPA dan 37 siswa, dilaksanakan melalui empat tahapan: tahap awal persiapan dan pretest, pembekalan teori, praktikum, dan tahap akhir posttest serta evaluasi kegiatan. Evaluasi hasil posttest menunjukkan terjadi peningkatan signifikan dalam pengetahuan dan pemahaman peserta, dari kategori "sangat kurang" dan "kurang" (19,56% dan 13,04%) pada pretest, menjadi "sangat baik" dan "baik" (34,78% dan 36,95%) pada posttest. Evauasi kegiatan, penilaian terhadap pemaparan materi dan kesesuaian antara teori dan praktik mencapai kategori sangat baik dengan persentase 80% dan 94%. Program pengabdian masyarakat ini terbukti efektif dalam meningkatkan kompetensi penggunaan mikroskop, pembuatan preparat, dan pengamatan sel, yang berkontribusi positif terhadap pembelajaran biologi di sekolah.
Co-Authors Ahadi Damar Prasetya Ari Sanjaya Aryani, Retno Didit Suprihanto Dwi Susanto Hetty Manurung Hetty Manurung, Hetty Imam Rosadi Indra Lesmana Iyut Jaya Toimsar Linda Oktavianingsih Linda Oktavianingsih Medi Hendra, Medi Niken Satuti Nur Handayani Nova Hariani Nur Miati Pratiwi, Anggita Endar Ratna Kusuma, Ratna Retno Aryani Rudianto Rudianto Rudy Agung Nugroho Samsurianto Samsurianto Wulan Iyhig Ratna Sari