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All Journal JURNAL BIOLOGI PAPUA
Eva S. Simaremare
Cenderawasih University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology

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Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua Verena Agustini; Eva S. Simaremare; Elsye Gunawan; Jane Awom; Susan Wopi
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.079 KB) | DOI: 10.31957/jbp.1289

Abstract

The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm. Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua. 
Phytochemical Screening and Antibacterial Activity of Dendrobium from Papua Against Eschericia coli and Staphylococcus aureus Supeni Sufaati; Verena Agustini; Agnes E. Maryuni; Eva S. Simaremare
Jurnal Biologi Papua Vol 13, No 2 (2021)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.774 KB) | DOI: 10.31957/jbp.1645

Abstract

Orchid found as one of medicinal plant in some areas, especially Dendrobium. Some species were investigated its bioactive compound, and antibacterial activity, but the information about antibacterial activity of Papuan Dendrobium species is still limited. The aims of this research were to determine the phytochemical constituents and the antibacterial activity of ethanolic extract and fraction of Dendrobium species from Papua against Escherichia coli and Staphylococcus aureus. There were three species of Dendrobium namely: D. spectabile, D. violaceoflavens, and D. antennatum used in this study. Dried simplisia was macerated using ethanol, then tested for the phytochemical content. Total ethanolic extract was fractionated with three different solvents, ethanol, ethyl acetate and hexane. Disc diffusion assay was used to examine the antibacterial activities of the total extract and the fractions. Total extract were positive for flavonoid, alkaloid and tanin, except D. spectabile stem extract only alkaloid and tannin.  No saponin was found in the plants. The ethyl acetate fraction of leaves of D. spectabile showed the highest antibacterial activity against S. aureus with diameter of inhibition zone 20.54 + 1.47 mm (strong category). Other plant extract and fraction tested had  moderate antibacterial activities against both E.coli and S aureus  with diameter zone  6-9 mm. The present work indicates that the ethyl acetate fraction of D. spectabile leaves is potential to be developed in antibacterial drug design research.  Key words: antibacterial activity; Papua; Dendrobium; E. coli; S. aureus. 
Co-Authors Agnes E. Maryuni Elsye Gunawan Jane Awom Supeni Sufaati Susan Wopi Verena Agustini Verena Agustini