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All Journal JURNAL BIOLOGI PAPUA Bakti Hayati
Supeni Sufaati
Cenderawasih University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Materials Science & Nanotechnology Social Sciences

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Phytochemical Screening and Antibacterial Activity of Dendrobium from Papua Against Eschericia coli and Staphylococcus aureus Supeni Sufaati; Verena Agustini; Agnes E. Maryuni; Eva S. Simaremare
Jurnal Biologi Papua Vol 13, No 2 (2021)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.774 KB) | DOI: 10.31957/jbp.1645

Abstract

Orchid found as one of medicinal plant in some areas, especially Dendrobium. Some species were investigated its bioactive compound, and antibacterial activity, but the information about antibacterial activity of Papuan Dendrobium species is still limited. The aims of this research were to determine the phytochemical constituents and the antibacterial activity of ethanolic extract and fraction of Dendrobium species from Papua against Escherichia coli and Staphylococcus aureus. There were three species of Dendrobium namely: D. spectabile, D. violaceoflavens, and D. antennatum used in this study. Dried simplisia was macerated using ethanol, then tested for the phytochemical content. Total ethanolic extract was fractionated with three different solvents, ethanol, ethyl acetate and hexane. Disc diffusion assay was used to examine the antibacterial activities of the total extract and the fractions. Total extract were positive for flavonoid, alkaloid and tanin, except D. spectabile stem extract only alkaloid and tannin.  No saponin was found in the plants. The ethyl acetate fraction of leaves of D. spectabile showed the highest antibacterial activity against S. aureus with diameter of inhibition zone 20.54 + 1.47 mm (strong category). Other plant extract and fraction tested had  moderate antibacterial activities against both E.coli and S aureus  with diameter zone  6-9 mm. The present work indicates that the ethyl acetate fraction of D. spectabile leaves is potential to be developed in antibacterial drug design research.  Key words: antibacterial activity; Papua; Dendrobium; E. coli; S. aureus. 
Peningkatan Kualitas Siswa dalam Pembelajaran Proyek Penguatan Profil Pelajar Pancasila (P5) Melalui Usaha Budidaya Jamur Tiram Putih (Pleurotus ostreatus) Supeni Sufaati; Suharno Suharno; Rosye H.R. Tanjung; Yayan Sopian; Totok A. Mushid; Juwita Juwita; Parjan Parjan; Eni Miton Busup; Nelince Fakdawer
Bakti Hayati: Jurnal Pengabdian Indonesia Vol. 2 No. 2 (2023)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/bhjpi.v2i2.3221

Abstract

One of the implementations of the Merdeka Curriculum is the Pancasila Student Profile Strengthening Project (P5). The implementation of P5 at PGRI High School in Jayapura City for grade X students in the 2022-2023 school year carries the theme of Entrepreneurship with a focus on oyster mushroom cultivation. The purpose of this activity is to provide understanding, practice, and business of white oyster mushroom cultivation at PGRI High School in Jayapura City. The methods used include Focus Group Discussion, Participatory Planning and small-scale cultivation practices at the school level. The results of the activity showed that the students were very enthusiastic in participating in the activity, especially during the practice of white oyster mushroom cultivation. After this activity there was an increase in student knowledge about white oyster mushroom by 57.5% and its nutritional content (61%). In addition, the participants also became aware of the economic value of white oyster mushroom. Furthermore, 79.17% of students have understood how to cultivate white oyster mushroom. In general, 87.5% of students were happy to participate in this activity. Moreover, the students have succeeded in cultivating white  oyster mushroom independently with simple technology at school. The white oyster mushroom cultivation activity is very interesting for students to learn entrepreneurship, in accordance with the P5 theme at the school. 
Co-Authors Agnes E. Maryuni Eni Miton Busup Eva S. Simaremare Juwita Juwita Nelince Fakdawer Parjan Parjan Rosye H.R. Tanjung Suharno Suharno Totok A. Mushid Verena Agustini Yayan Sopian