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Cytotoxic Activity and Phytochemical Analysis of Breynia cernua from Papua Dirgantara, Septriyanto; Tanjung, Rosye H.R.; Maury, Hendra K.; Meiyanto, Edy
Indonesian Journal of Pharmaceutical Science and Technology Suppl 1, No. 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.159 KB) | DOI: 10.15416/ijpst.v1i1.16121

Abstract

Breynia cernua (local name Katuk Hutan) is an Indonesian medicinal plant (family: Euphorbiace- ae) originated from Papua which have been used traditionally as alternative treatment for breast and cervical cancer. The objectives of this research were to investigate potential cytotoxic activity and phytochemical aspect test of B. cernua extract and its fractions. Extraction was performed by macera- tion using ethanol 96%, followed by successive fractionations. Phytochemical screening and in vitro cytotoxic activity test were obtained for extract and active fraction with Brine Shrimp Lethality Test (BSLT) method and MCF-7 tumor cell lines were assessed by 3-(4-5-dimethylthiazol-2yl)-2,5-diphe- nyl tetrazolium bromide (MTT) assay. The results from cytotoxic activity test of breast cancer MCF-7 cell line from ethanolic 96% extract and three fractions: n-hexane, ethylacetate and water fractions showed 246.841 ppm, 165.65 ppm, 562.57 ppm dan 713.78 ppm respectively meanwhile the IC50 value of doxorubicin as the positive control was 6 μM. TLC and phytochemical screening showed the pres- ence of alkaloids, terpenoid, avonoids and tannins compounds in this active plant. Keywords: Antitumor, Breynia cernua, BSLT, MCF-7
Arbuscular Mycorrhizal Fungi Associated with Wati (Piper methysticum), a Medicinal Plant from Merauke Lowland, Papua, Indonesia Suharno, Suharno; Sufaati, Supeni; Agustini, Verena; Tanjung, Rosye H. R.
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14303

Abstract

Indonesia is rich of natural resources, including the diversity of agricultural crops and other valuable plants. Many plant species used by local people for traditional herbal medicine and some other species used by drug industries. Among these, Wati (Piper methysticum, Piperaceae) is one of the medicinal plant found in Merauke lowland, Papua. It has been cultivated by local people because of it high value as medicinal and cultural uses. Wati plant is used to treat anti-stress, rheumatism, respiratory tract infections, tuberculosis, gonorrhea, headache etc. The habitat, including the microorganism in the soil plays an important role in the growth of this plant. Therefore, this study was conducted to identify the arbuscular mycorrhizal fungi (AMF) associated with the rhizosphere of Wati from Merauke lowland. Soil and root samples were collected from different locations and the colonization percentage on the root sample were determined. Our results showed that the number of the spores in the soil samples was 45-89 spores/50 g soil, while the colonization percentage on the root was 38.46-83.3%. Among 13 AMF morphospecies that found on the soil samples, 10 were identified to genus level such as Glomus, Clariodeoglomus, Acaulospora and Scutellospora, while the other were unidentified. Further work will be needed such as trap-plant culture method to get more information on the diversity of AMF associated with Wati.
FERMENTASI AMPAS SAGU (FAS) SEBAGAI PAKAN ALTERNATIF UNTUK MENINGKATAN PERTUMBUHAN BOBOT AYAM KAMPUNG Saebu, Yusuf; Tanjung, Rosye H.R.; Suharno, _
Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Volume 5, Nomor 1, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.1.2020.%p

Abstract

Sagu (Metroxylon sagu) merupakan salah satu tanaman kelompok palm yang mengandung zat pati tinggi, dan telah dimanfaatkan sebagai bahan pangan pokok bagi masyarakat Papua. Semua bagian dari tanaman ini dimanfaatkan untuk kebutuhan manusia. Namun, ampas sebagai bagian dari sisa produk zat pati belum dimanfaatkan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh fermentasi ampas sagu (FAS) sebagai pakan alternatif untuk pertumbuhan ayam kampung. Penelitian ini dilakukan dengan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan, yaitu : P0 =Kontrol (PB 100% dan FAS 0%), P1 =PB 96% dan FAS 4%, P2 =PB 92% dan FAS 8%, dan P3 =PB 88% dan FAS 12%. Parameter yang diukur antara lain adalah tinggi badan dan bobot ayam. Analisis dilakukan pada ampas sagu baik sebelum fermentasi maupun setelah proses fermentasi. Hasil analisis menunjukkan bahwa FAS mengandung protein kasar, serat kasar, dan lemak kasar mengalami peningkatan masing-masing sebesar 66,5%, 21,1%, 54,5% dibandingkan sebelum dilakukan fermentasi. Perlakuan FAS tidak memberikan pengaruh terhadap peningkatan tinggi tubuh ayam kampung. Perlakuan signifikan nampak pada parameter bobot tubuh ayam     kampung. Peningkatan terbaik bobot ayam terjadi pada perlakuan P1 (PB 96% dan FAS 4%). Perlakuan P1 mampu meningkatkan bobot ayam hingga mencapai 17,8%, sedangkan P2 mencapai 10%. FAS dapat dimanfaatkan sebagai pakan alternatif dalam meningkatkan pertumbuhan bobot ayam kampung. Kata kunci : sagu, fermentasi, pertumbuhan, ayam kampung
Deteksi Perubahan Luasan Mangrove Teluk Youtefa Kota Jayapura Menggunakan Citra Landsat Multitemporal Baigo Hamuna; Rosye H.R. Tanjung
Majalah Geografi Indonesia Vol 32, No 2 (2018): Majalah Geografi Indonesia
Publisher : Fakultas Geografi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2826.777 KB) | DOI: 10.22146/mgi.33755

Abstract

Kondisi mangrove di kawasan Teluk Youtefa, baik dari aspek kualitas maupun kuantitasnya terus mengalami penurunan dari tahun ke tahun. Penelitian ini dilakukan untuk mengetahui seberapa besar perubahan luasan mangrove yang terjadi di kawasan Teluk Youtefa, Kota Jayapura dari tahun 1994 sampai tahun 2017 dengan menggunakan citra satelit Landsat 5 TM dan Landsat 8 OLI. Pengamatan kondisi mangrove di lapangan dilakukan dengan menggunakan GPS dan pengolahan citra menggunakan algoritma NDVI dengan klasifikasi supervised. Tumpang susun peta hasil interpretasi citra satelit untuk mengetahui sebaran dan perubahan luasan kawasan mangrove. Hasil penelitian menunjukan bahwa luasan mangrove pada tahun 1994 sebesar 392,45 ha dan luasan mangrove pada tahun 2017 mengalami penurunan menjadi 233,12 ha. Perubahan luasan mangrove dalam kurun waktu 23 tahun sebesar 159,34 ha atau sebesar 40,59%. Perubahan kawasan mangrove pada umumnya disebabkan oleh faktor antropogenik seperti penebangan, perubahan fungsi kawasan mangrove menjadi jalan, jembatan, pemukiman dan perubahan secara alami. ABSTRACTThe condition of mangrove in Youtefa Bay both qualitatively and quantitatively has decreased from year to year. This research was conducted to determine how much of the change occurring mangrove area in Youtefa Bay, Jayapura City from 1994 to 2017 by using Landsat 5 TM images and Landsat 8 OLI. Monitoring of mangrove condition in the field used GPS, and processing of images used NDVI algorithm with supervised classification. The map was overlaying satellite imagery interpretation to determine the distribution and changes of mangrove area. The result of research showed that the mangrove area in 1994 was about 392.45 hectares, mangrove area in 2017 have decreased becoming was 233.12 hectares. Changing of mangrove area for 23 years was about 159.34 hectares or 40.59%. Changes in mangrove were generally caused by anthropogenic factors such as logging, changes over the function of mangroves into the road, bridge, settlement, and change naturally.
FUNGI MIKORIZA ARBUSKULA (FMA) YANG BERASOSIASI DENGAN Durio zibethinus DI KABUPATEN MANOKWARI, PAPUA BARAT, INDONESIA Suharno Suharno; Verena Agustini; Rosye H.R. Tanjung; Supeni Sufaati
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 2 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2019.13.2.61-69

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Arbuscular mycorrhizal fungi (AMF) is a symbiosis between fungi and plants. This association is mutualism, AMF contributes to increased plant growth while fungi obtain energy sources from plant assimilation. The purpose of this study was to determine the association of AMF with durian plants (Durio zibethinus) in Manokwari, West Papua. The survey was conducted in 10 villages located in 4 districts known as the center for producing durian. Observation of the presence of AMF spores in the plant's rhizosphere was carried out by the wet sieving method. Furthermore, to find out the association between the two symbionts is done by observing AMF colonization in plant roots. The types of AMF found in the plant's rhizosphere are morphologically identified based on spore characteristics. The results showed that durian was associated with AMF. The percentage of AMF colonization ranged from 39.29 to 80.00%, while the number of spores was 112-336 spores per 100 grams of soil sample. Based on the spore morphological characteristics, AMF found is dominated by the genus Glomus, Scutellospora, and Acaulospora.  
Method of Approach to Application Stages and Procedures for Implementing Itchy Leaves Product Eva Susanty Simaremare; Rosye Tanjung; Yuliana Ruth Yabansabra; Elsye Gunawan
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 5, No 2 (2019): Agustus
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.125 KB) | DOI: 10.22146/jpkm.39763

Abstract

Depapre District is overgrown with itchy leaves which are useful as traditional medicines, for pain, fatigue, aches. This itchy leaf is widely available in the village and is used but has not been maximally managed. If itchy leaves are used, the economy of the community can be helped. The purpose of this activity is to implement the DPTM program which were to socialize, train, create, and evaluate the diversification of itchy leaf products. This activity also provided materials, supporting facilities and infrastructure in making itchy leaf products to the Depapre District community by involving the district government, the Indonesian Parliament and the BPOM as the relevant agencies. The method was carried out by means of coordination, implementation of activities in the form of lecture and lecture, practice of making itchy leaf products, and evaluation. This activity was carried out for 6 months. The conclusion obtained from this activity is that 51% of participants have participated in the same activity. As many as 98, 9% of participants benefited from this activity and 85% of the community were able to make itchy leaf products. There were three grinding technology units and three boxes of chemicals that are donated to the people of Depapre, each of which contains a device (stamper and mortar), materials (PEG 400, PEG 6000, methyl paraben, palm oil, talc, powder containers, labels, simplicia container, ointment container). In this activity also produced itchy leaf products in the form of simplicia and ointments.
New Record of Arbuscular Mycorrhizal Fungi (AMF) Association with Kebar Grass (Biophytum petersianum Klotzsch.) in the Grassland Area of Kebar, Tambrauw Regency, West Papua, Indonesia Suharno Suharno; Irma Rahayu; Rosye H. R. Tanjung; Supeni Sufaati
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.70021

Abstract

Arbuscular mycorrhizal fungi (AMF) are an important form of symbiosis between fungi and plants in an ecosystem. One of the medicinal plants used by the people in West Papua is kebar grass (Biophytum petersianum Klotzsch.). This study aims to determine the AMF association in the rhizosphere of B. petersianum in grasslands. Survey method was used in this study. The presence of AMF was observed by examining root colonization and spore diversity. The results showed that the percentage of AMF colonization in roots was between 46.7–90.0% with an average of 71.66%. Meanwhile, the number of spores found in the plant rhizosphere averaged 119.8 spores per 10 grams of soil sample. There were 18 species of AMF dominated by the genus Glomus (7 species), Acaulospora (3 species), while the genus Claroideoglomus, Entrophospora, Gigaspora, and Scutellospora were dominated each with 2 species. This finding is the first record on the presence of AMF on B. petersianum in West Papua.
Kajian Kualitas Air Laut dan Indeks Pencemaran Berdasarkan Parameter Fisika-Kimia di Perairan Distrik Depapre, Jayapura Baigo Hamuna; Rosye H.R. Tanjung; Suwito Suwito; Hendra Kurniawan Maury; Alianto Alianto
Jurnal Ilmu Lingkungan Vol 16, No 1 (2018): April 2018
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.41 KB) | DOI: 10.14710/jil.16.1.35-43

Abstract

ABSTRAKKondisi kualitas air suatu perairan yang baik sangat penting untuk mendukung kelulushidupan organisme yang hidup di dalamnya. Penentuan status mutu air perlu dilakukan sebagai acuan dalam melakukan pemantauan pencemaran kualitas air. Penelitian ini bertujuan untuk mengkaji status mutu air dan menentukan indeks pencemaran berdasarkan parameter fisika-kimia di perairan Distrik Depapre, Kabupaten Jayapura. Pengambilan sampel kualitas air dilakukan pada bulan Oktober 2017 di lima stasiun penelitian, kemudian hasilnya dibandingkan dengan baku mutu air laut untuk biota laut berdasarkan KEPMEN-LH No. 51 Tahun 2004. Hasil penelitian menunjukkan bahwa parameter yang masih sesuai baku mutu antara lain suhu, salinitas, sulfida dan kecerahan perairan, sedangkan parameter yang telah melampaui baku mutu antara lain pH, ammonia total, nitrat dan fosfat. Berdasarkan hasil perhitungan indeks pencemaran menunjukkan bahwa perairan Distrik Depapre berada dalam kategori tercemar ringan hingga tercemar sedang.Kata kunci: Baku Mutu, Indeks Pencemaran, Kualitas Air, Parameter Fisika-Kimia, Distrik DepapreABSTRACTGood water quality is extremely important to support life of organisms. The determination of water quality status was needed as reference to monitor water pollution. This study aimed to assess the status of water quality and determine pollution index based on physical-chemical parameters in the Depapre District waters, Jayapura Regency. Sampling was carried out in October 2017 across five research stations, then the results were compared with water quality standards based on KEPMEN-LH No. 51 Tahun 2004 for marine biotas. The results showed that the parameters in according to the quality standards are temperature, salinity, sulphide and water transparency, while those that have exceeded the quality standards are pH, total ammonia, nitrate and phosphate. Based on the calculation of pollution index indicates that the Depapre District waters was in light pollution to medium categories.Keywords: Depapre District, Physical-Chemical Parameters, Pollution Index, Standards, Water QualityCitation: Hamuna, B., Tanjung, R.H.R, Suwito, Maury H.K. dan Alianto (2018).Kajian Kualitas Air Laut dan Indeks Pencemaran Berdasarkan Parameter Fisika-Kimia Di Perairan Distrik Depapre, Jayapura. Jurnal Ilmu Lingkungan, 16(1), 35-43, doi:10.14710/jil.16.135-43
ANALISIS TINGKAT KERUSAKAN DAN KONDISI EKOLOGI EKOSISTEM MANGROVE MIMIKA PAPUA John D. Kalor; Rosye H.R. Tanjung; Ervina Indrayani; Kristopholus Rumbiak
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 1, No 1 (2018)
Publisher : ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.684 KB) | DOI: 10.31957/.v1i1.502

Abstract

Penelitian ini dilakukan untuk mengetahui status kerusakan dan kondisi ekosistem mangrove di Kabupaten Mimika. Pengambilan data dilakukan tanggal 13- 21 Oktober 2015, pada 6 stasiun di Pesisir Selatan Mimika yakni (1) Stasiun Manasari, (2) St. Kampung Atakwa, (3) St. Kampung Atukwa, (4) St. Kakonau, (5) St. Kampus Biru, dan (6) St. Pomako. Menggunakan transek garis dan petak contoh yang dibuat tegak lurus garis pantai di daerah intertidal. Data dianalisis menggunakan Indeks Nilai Penting dan Uji Kriteria Kerusakan berdasarkan kepadatan vegetasi. Penelitian ini menemukan Kabupaten Mimika memiliki status ekosistem mangrove yang baik-sangat baik, namun dibeberapa lokasi terdapat kerusakan yang menyebabkan kerapatan menjadi rendah.Kata Kunci: Kerusakan, Ekologi, Mangrove, Mimika, Papua
STUDI KARAKTERISTIK PASANG SURUT PERAIRAN LAUT MIMIKA, PROVINSI PAPUA Baigo Hamuna; Rosye H.R. Tanjung; John D. Kalor; Lisiard Dimara; Ervina Indrayani; Maklon Warpur; Yunus Y.P. Paulangan; Kalvin Paiki
ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua Vol 1, No 1 (2018)
Publisher : ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.464 KB) | DOI: 10.31957/.v1i1.503

Abstract

Salah satu karakteristik perairan Mimika adalah banyaknya sungai-sungai besar yang bermuara di wilayah perairan Mimika yang mempengaruhi berbagai aktivitas, salah satunya adalah aktivitas transportasi kapal berukuran besar yang akan masuk dan keluar di pelabuhan Poumako dan Port Site-Freeport harus melalui sungai dan sangat bergantung pada proses pasang surut air laut. Penelitian ini bertujuan untuk mengetahui dan mengkaji karakteristik pasang surut di perairan Mimika, Provinsi Papua. Data yang digunakan adalah data pasang surut selama 29 hari dengan interval pengamatan 1 jam. Penentuan tipe pasang surut dan tinggi muka air rata-rata dengan menggunakan metode Least-Squares. Hasil pengolahan data menunjukkan bahwa tipe pasang surut di perairan Mimika adalah pasang surut campuran condong ke harian tunggal dengan bilangan Formzahl 2,9498. Hal ini berarti bahwa pada perairan Mimika dalam sehari terjadi satu kali atau dua kali pasang dengan interval tinggi air laut yang berbeda. Adapun nilai komponen-komponen elevasi muka air pada periode pengamatan yang meliputi HHWL (4,3153 m), MHWL (2,4476 m), MSL (1,7996 m), MLWL (0,9938 m), dan LLWL (0,3102 m).Kata Kunci: Metode Least-Squares, Formzahl, pasang surut, Perairan Mimika 
Co-Authors Agamawan, Lalu Panji I. Agnes Kabelen Agnes Kabelen, Agnes Akrom, Akrom Alfred A Antoh Ali Djamhuri Alianto , Baigo Hamuna Baigo Hamuna Baigo Hamuna Baigo Hamuna Baigo Hamuna Basa T Rumahorbo Cahyaningsih, Ayu Daniel Lantang Dewi Sulistiowati Dwi Haryanto Dwi Haryanto Edy Meiyanto Efray Wanimbo Elsye Gunawan Eni Miton Busup Erni Unenor Erni Unenor, Erni Ervina Indrayani Fali Sembori Fali Sembori Floriana Kabelen Hanna E. Samosir Helena G Burok Helena G. Burok Henderina J. Keiluhu Henderina J. Keiluhu Hendra Kurniawan Maury Ign. Joko Suyono Irma A. Resmol Irma Rahayu Irma Rahayu, Irma Jane T. Sada Jane T. Sada Jhon D. Kalor Jhon D. Kalor John D Kalor Juwita Juwita Kabes, Wetty Queenisa Kalvin Paiki Komari Komari Konstantina M.B. Kameubun Konstantina M.B. Kameubun Kristopholus Rumbiak Lantang , Daniel Lisiard Dimara Lisye I. Zebua Lisye I. Zebua Lisye I. Zebua Lisye Iriana Zebua Lisye Iriana Zebua Lucia Runggeari Lucia Runggeari Mahmudi Mahmudi Maklon Warpur Manalu, Khristhoper Aris A. Manwan, Sri Wahyuni Marcelino N. Yonas Marthen L.R. Bartholomeus Mathen L.R. Bartholomeus Meidy J. Imbiri Mesak Kamarea Mesak Kamarea Mesak Kamarea Nelince Fakdawer Nurtanti HAndono Nurtanti Handono Parjan Parjan Pulle, Yosevin H. Purwanto Purwanto Purwanto Purwanto R. Rijanta Rejauw, Korinus Rini Patandungan Rini Patandungan Runtuboi, Dirk Yanes Saebu, Yusuf Sammaria J. Karma Septriyanto Dirgantara Sipriana Dogomo Siregar, Martha D. Suharno Suharno Suharno Suharno Suharno Suharno Suharno Suharno Suharno, _ Supeni Sufaati Supeni Sufaati Supeni Sufaati Supeni Sufaati Supeni Sufaati Supeni Sufaati Suriani B.R. Surbakti Suriani Br. Surbakti Suwito Suwito Suwito Suwito Suwito Suwito Totok A. Mushid Tri Gunaedi Verena Agustini Verena Agustini Verena Agustini Verena Agustini Wahyu Dwi Nugroho Wigati Yektiningtyas Wigati Yektiningtyas Yan P. Yepese Yan Piet Yepese Yayan Sopian Yemeus Helakombo Yuliana Ruth Yabansabra Yuliana Yuliana Yulius Sarungu Yunus Pajanjan Paulangan Ziyadatun Ni'mah