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All Journal BERITA BIOLOGI Omni-Akuatika Jurnal Riset Akuakultur JIPI (Jurnal Ilmiah Penelitian dan Pembelajaran Informatika)
Taukhid Taukhid, Taukhid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science

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APLIKASI VAKSIN Streptococcus agalactiae UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) [Application of Streptococcus agalactiae vaccine to prevent streptococcosis on tilapia culture, Oreochromis niloticus] Taukhid, Taukhid; Lusiastuti, Angela Mariana; Sumiati, Tuti
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.857 KB) | DOI: 10.14203/beritabiologi.v13i3.662

Abstract

The research with the aim to know the effectivity (yield gap) of the application of Streptococcus agalactiae vaccine (pure whole cell) in prevention of streptococcosis on tilapia (Oreochromis niloticus) culture has been carried out. The isolate of S.agalactiae – N14G was used as a master seed on vaccine production. Priming vaccination was administered by immersion method, and booster vaccination was taken th place two months latter by oral method. Challenge test at the lethal dose (LD50) against active bacteria was done at 14 days post booster vaccination, and observation was taken place for 14 days post artificial infection. The results of the research showed that the highest survival rate and relative percent survival (RPS) was found in group treated with Streptovac vaccine (S. agalactiae and A. hydrophila combination) (65.58% and 35.36%) followed by S. agalactiae vaccine (52.08% and 10.01%). The lowest survival rate was found in control group (46.75%). The result of confirmation effectivity of the vaccines by challenge test in the laboratory showed that the highest survival rate and relative percent survival (RPS) was found in S. agalactiae vaccine (50.00% dan 37.50%) followed by Streptovac vaccine (40.00% and 25.00%), and the lowest survival rate was found in control group (20.00%). Vaccination is better than the non vaccinated.
OPTIMASI FREKUENSI PEMBERIAN VITAMIN C PAD A PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPES VIRUS (KHV) PADA IKAN MAS (Cyprinus carpio Linn.) Taukhid, Taukhid; Lusiastuti, Angela Mariana; Suryadi, Kusumasari; Rosidah, Rosidah; Setiadharma, Gunawan
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.665 KB) | DOI: 10.14203/beritabiologi.v10i3.749

Abstract

The research with objective to understand optimization frequency of supplemented ascorbic acid (microencapsulated vitamin C CFC-90) feeding to control the Koi Herpes Virus (KHV) disease infecting common carp has been done in Fish Disease Laboratory Fishes were reared in plastic container (80 litres), with density of 20 fish sized 10 gram in average. The treatments were: (A) daily application, (B) three daily application, (C) five daily application, and (D) without vitamin C as a control. Examined fishes were challenged to KHV infection after the 21 days rearing period by cohabitation method for 2 weeks. Observations been done on behaviour, clinical signs and mortality of fishes. The results showed that the highest survival rate was found on the application o vitamin C given every 3 days (50.0%); followed by every day (12.5%), every 5 days (7.5%), and the lowest was found on contro group (1.3%). Control techniques in the case of KHV carp populations through the provision of vitamin C immunostimulatory conducted regularly since well before the existence of KHV infection provides the best protective level.
Streptococcosis on Nile tilapia (Oreochromis niloticus) in Indonesian freshwater aquaculture Taukhid, Taukhid; Wajdy, Edy Farid; Sugiani, Desy; Nafiqoh, Nunak
Journal Omni-Akuatika Vol 19, No 1 (2023): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2023.19.1.1005

Abstract

This review aimed to provide the current status of streptococcosis in freshwater aquaculture in Indonesia. So far, this disease has been mostly reported in tilapia aquaculture, and there have been no serious reports of this disease in other species of freshwater aquaculture. The disease is mostly encountered during the dry season or the transition period from the dry season to the rainy season or vice versa. Initially, the disease was mostly reported in tilapia larger than 250 grams, but in its development, it was also reported in smaller fish. The Ministry of Marine Affairs and Fisheries (MMAF) has classified this disease as a major disease in tilapia in Indonesia since 2014. Based on the characteristics of Streptococcus agalactiae isolates collected from diseased tilapia from various regions in Indonesia, two biotypes were found consisting of ?-hemolytic S. agalactiae (biotype I) and non-hemolytic S. agalactiae (biotype II). Biotype I cause disease with acute mortality patterns, while biotype II causes disease with chronic and persistent mortality patterns. Based on susceptibility to S. agalactiae during artificial infection testing, it is known that all tilapia strains that have been released to the public for aquaculture purposes are susceptible to infection with the bacteria; although each strain has a different level of susceptibility. Fish health management strategies that are based on preventive measures and relevant to sustainable aquaculture programs should be based on ecological-biological approaches such as the implementation of biosecurity systems, the use of vaccines for endemic diseases, probiotics, essential herbs, and the development of more disease-resistant populations.Keywords: health management, streptococcosis, Streptococcus agalactiae, tilapia
Susceptibility of three indigenous Indonesian fish species: mahseer (Tor soro), snakehead (Channa striata), and bagrid catfish (Hemibagrus nemurus) against parasites infection Sumiati, Tuti; Taukhid, Taukhid; Andriyanto, Septiyan; Wadjdy, Edy Farid
Journal Omni-Akuatika Vol 18, No 2 (2022): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2022.18.2.1000

Abstract

Abstract Mahseer "dewa" (Tor soro), snakehead "gabus" (Channa striata), and bagrid catfish "baung" (Hemibagrus nemurus) are local specific fish species that are promising to be developed as freshwater aquaculture commodities in Indonesia. Disease cases/outbreaks in those fish farming have often been reported, however, there is not much information on the epizootiology and disease status of those fish species. The susceptibility of those fish to parasitic infection was evaluated by natural and artificial infection. Natural infection was conducted by randomly sampling 20 fish at 5-day intervals and lasting for 30 days. In artificial infection, 150 tested fish cohabited with 40 parasite-carrying fish that were known to be definitely infected by the parasite. The observation was carried out by sampling 20 fish at 5-day intervals and lasting for 30 days. The results showed that the three fish species were susceptible to infection with Trichodina spp, Epistylis spp, Tetrahymena spp, Ichtyophthirius multifiliis, Dactylogyrus spp, and Gyrodactylus spp, while the helminth parasite Pallisentis nagpurensis (Acanthocephala) was only identified in snakehead. A number of parasites such as I. multifiliis, Dactylogyrus spp., and Gyrodactylus spp. are likely to be potential obstacles in the cultivation of those fish species, especially in hatcheries and nurseries. Keywords: mahseer, snakehead, bagrid catfish, parasites
EFFECTIVENESS OF E-LEARNING MEDIA DEVELOPMENT USING CANVA IN THE PJBL MODEL Taukhid, Taukhid; Prasetya, Taufan Agung; Anggoro, Sapto Dwi
JIPI (Jurnal Ilmiah Penelitian dan Pembelajaran Informatika) Vol 10, No 3 (2025)
Publisher : STKIP PGRI Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29100/jipi.v10i3.8969

Abstract

The purpose of this research is to develop Canva-based learning media using the Project-Based Learning model in Pancasila Education at STIKES Hang Tuah Surabaya that is valid and practical. The method used in this research and development is the ADDIE model. Data were obtained from validation questionnaires and practicality question-naires. The research subjects consisted of 3 expert validators covering media, language, and content, while peer testing involved 1 person, and 23 nursing students from STIKES Hang Tuah Surabaya. The results of the media development research showed that the content validity level was 90 percent categorized as very valid, language validity was 95 per-cent categorized as very valid, and media validity was 98 percent cate-gorized as very valid. Furthermore, the practicality response from stu-dents was 89.31 percent categorized as very practical, while the feed-back from educators showed 91.66 percent categorized as very practi-cal. The results of the study found that the Canva-based learning media using the Problem-Based Learning model is very valid and practical for use in Pancasila education for nursing students at STIKES Hang Tuah Surabaya.
PERFORMA VAKSIN IKAN ‘TRIVALEN-SA’ PASCAPENYIMPANAN SELAMA 12 BULAN UNTUK PENCEGAHAN KO-INFEKSI STREPTOCOCCOSIS DAN MOTILE AEROMONAS SEPTICEMIA PADA IKAN NILA (Oreochromis niloticus) Kholidin, Edy Barkat; Wadjdy, Edy Farid; Sudarmaji, Sudarmaji; Sirodiana, Sirodiana; Abduh, Muhammad; Saputra, Adang; Murniasih, Siti; Taukhid, Taukhid
Jurnal Riset Akuakultur Vol 19, No 4 (2024): Desember (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.4.2024.277-298

Abstract

Riset ini bertujuan untuk mengevaluasi efikasi dan keamanan vaksin ikan ‘Trivalen-SA’ pascapenyimpanan selama 12 bulan untuk pencegahan ko-infeksi streptococcosis dan motile Aeromonas septicemia (MAS) pada ikan nila (Oreochromis niloticus). Ikan uji yang digunakan adalah ikan nila yang sudah specific pathogen free dengan bobot 8 ± 1,2 g. Perlakuan yang diberikan adalah aplikasi vaksin melalui: (1) Injeksi satu dosis (0,1 mL per ekor), (2) Injeksi dua dosis (0,2 mL per ekor), (3) Perendaman dalam larutan vaksin pada konsentrasi 107 CFU mL-1,(4) Injeksi 0,1 mL phosphate buffered saline (PBS) sebagai kontrol positif, dan (5) Perendaman dalam air segar selama 30 menit sebagai kontrol negatif.  Efikasi vaksin dievaluasi dengan nilai relative percent survival (RPS) melalui uji tantang terhadap kedua jenis bakteri infektif penyusun formula vaksin. Uji keamanan vaksin dilakukan sesuai metode standar pengujian vaksin ikan. Hasil penelitian menunjukkan bahwa vaksin ikan ‘Trivalen-SA’ pada pengujian ini masih aman dan protektif dengan hasil secara kuantitatif lebih baik karena nilai RPS terhadap infeksi bakteri Aeromonas hydrophila berkisar antara 15,79-29,82%; bakteri Streptococcus agalactiae (non-hemolitik) antara 25,92-48,15%, dan bakteri S. agalactiae (β-hemolitik) antara 18,52-40,75%. Proteksi sinergis terhadap ko-infeksi ketiga jenis atau biotipe bakteri adalah 27,37% untuk injeksi satu dosis; 39,57% untuk injeksi dua dosis, dan 22,54% untuk perendaman. Hasil terbaik berdasarkan kinerja sintasan dan pertambahan bobot tubuh ikan nila dengan nilai RPS mencapai 39,57% apabila diberikan vaksin melalui injeksi dua dosis (0,2 mL per ekor).A study was conducted to determine the efficacy and safety of the ‘Trivalen-SA’ fish vaccine after 12 months of storage in preventing co-infection by streptococcosis and motile Aeromonas septicemia (MAS) in tilapia (Oreochromis niloticus). Specific pathogen-free tilapia with a weight of 8 ± 1,2 g were used as the test fish. The treatments included: (1) Injection with a single dose (0.1 mL per fish), (2) Injection with a double dose (0.2 mL per fish), (3) Immersion with the vaccine solution in 107 CFU mL-1, (4) Injection with 0.1 mL phosphate buffered saline (PBS) as a positive control, and (5) Immersion with freshwater for 30 minutes as a negative control. Vaccine efficacy was assessed using relative percent survival (RPS) values obtained from the challenge tests against each bacterial species in the vaccine. Vaccine safety was evaluated according to standard fish vaccine testing protocols. The results showed that the ‘Trivalen-SA’ vaccine in this study remained safe and effective indicated by RPS value against Aeromonas hydrophila ranged from 15.79 to 29.82%, non-hemolytic Streptococcus agalactiae ranged from 25.92 to 48.15%, and β-hemolytic S. agalactiae ranged from 18.52 to 40.75%. The synergistic protection against co-infection by all three bacterial species or biotypes was 27.37% for the single-dose injection, 39.57% for the double-dose injection, and 22.54% for the immersion method.  The best results based on the survival performance and body weight gain of tilapia with an RPS value reached 39.57% when given the vaccine through double-dose injection (0.2 mL per fish).
Co-Authors Andriyanto, Septiyan ANGELA MARIANA LUSIASTUTI Kholidin, Edy Barkat Muhammad Abduh Murniasih, Siti Nafiqoh, Nunak Prasetya, Taufan Agung Rosidah Rosidah Sapto Dwi Anggoro, Sapto Dwi Saputra, Adang Setiadharma, Gunawan Sirodiana, Sirodiana SUDARMAJI SUDARMAJI Sugiani, Desy Suryadi, Kusumasari Tuti Sumiati, Tuti Wadjdy, Edy Farid Wajdy, Edy Farid