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S. Sunardhi Widyaputra, S. Sunardhi
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Immunoexpression of cytokeratin 19 in oral swab from fixed orthodontic appliance users Ramdhini, Dwi Mutia; Widyaputra, S. Sunardhi; Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 19, No 3 (2007): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2169.405 KB) | DOI: 10.24198/pjd.vol19no3.14156

Abstract

The use of fixed orthodontic appliances can improve someones mastication, speech and appearance. However, this appliance acts as a strange object that may cause irritation to the mucosa epithelial of oral cavity, because of the friction and pressure from the components of the fixed orthodontic appliances which are in direct contact with the oral mucosa. Irritation in the oral mucosa could stimulate the increase of cytokeratin. The appearance of cytokeratin is then used to identify the condition of these cells. This study was a descriptive research to find the expression of cytokeratin 19 with immunohistochemical method in oral mucosa epithelial of fixed orthodontic appliances users. Sample in this study was chosen from 30 fixed orthodontic appliances users. The result of this study was determined by calculating the number of positive cells (brown), compared with total number of cells. The account of positive cells would present the reaction of the epithelial cells according to the inflamation stage which caused by the use of orthodontic appliances. As a conclusion of this study, the use of fixed orthodontic appliances may cause changes in epithelial mucosa which form an adaptation process by increasing the number of progenitor cells marked by cytokeratin 19.
Tissue reaction against implantation of nanocomposite and giomers Vilvarajah, Anusha S; Djustiana, Nina; Widyaputra, S. Sunardhi
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3007.105 KB) | DOI: 10.24198/pjd.vol23no1.14057

Abstract

Dentistry today has inherited technological advancement from other Dental Material sciences, examples are Nanocomposite and Giomers. Nanocomposites and Giomers are common materials used in Dentistry. But what are the implications when these materials are used in practice? The aim for this the study shows the analysis of tissue reaction due to implantation of Nanocomposite and Giomers. The subcutaneous tissue of a mouse is substituted with the human gum tissues. In the experimental group, Nanocomposite and Giomers were implanted in the subcutaneous tissue abdomen region in mice. The slides were made from the surrounding of implantation for both experimental and control groups. The evaluation of the effects of the implant is done in a time interval. Evaluated time intervals are 24 hours, 7" day, 14th day, 21st day and 28th day respectively. The amounts of inflammatory cells formation in both groups were compared.Once the results of the inflammatory cells are evaluated in the given time interval for Nanocomposite, Giomer, and control group then they are statistically analyzed. The statistics used in the experiments is Mann-Whitney and Wilcoxon. The conclusion of this research showed that statistically significant differences on lymphocytes value between treatment and control group.
Tissue reaction against implantation of nanocomposite and giomers Vilvarajah, Anusha S; Djustiana, Nina; Widyaputra, S. Sunardhi
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3007.105 KB) | DOI: 10.24198/pjd.vol23no1.14057

Abstract

Dentistry today has inherited technological advancement from other Dental Material sciences, examples are Nanocomposite and Giomers. Nanocomposites and Giomers are common materials used in Dentistry. But what are the implications when these materials are used in practice? The aim for this the study shows the analysis of tissue reaction due to implantation of Nanocomposite and Giomers. The subcutaneous tissue of a mouse is substituted with the human gum tissues. In the experimental group, Nanocomposite and Giomers were implanted in the subcutaneous tissue abdomen region in mice. The slides were made from the surrounding of implantation for both experimental and control groups. The evaluation of the effects of the implant is done in a time interval. Evaluated time intervals are 24 hours, 7" day, 14th day, 21st day and 28th day respectively. The amounts of inflammatory cells formation in both groups were compared.Once the results of the inflammatory cells are evaluated in the given time interval for Nanocomposite, Giomer, and control group then they are statistically analyzed. The statistics used in the experiments is Mann-Whitney and Wilcoxon. The conclusion of this research showed that statistically significant differences on lymphocytes value between treatment and control group.
Immunoexpression of cytokeratin 19 in oral swab from fixed orthodontic appliance users Ramdhini, Dwi Mutia; Widyaputra, S. Sunardhi; Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 19, No 3 (2007): November 2007
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2169.405 KB) | DOI: 10.24198/pjd.vol19no3.14156

Abstract

The use of fixed orthodontic appliances can improve someone's mastication, speech and appearance. However, this appliance acts as a strange object that may cause irritation to the mucosa epithelial of oral cavity, because of the friction and pressure from the components of the fixed orthodontic appliances which are in direct contact with the oral mucosa. Irritation in the oral mucosa could stimulate the increase of cytokeratin. The appearance of cytokeratin is then used to identify the condition of these cells. This study was a descriptive research to find the expression of cytokeratin 19 with immunohistochemical method in oral mucosa epithelial of fixed orthodontic appliances users. Sample in this study was chosen from 30 fixed orthodontic appliances users. The result of this study was determined by calculating the number of positive cells (brown), compared with total number of cells. The account of positive cells would present the reaction of the epithelial cells according to the inflamation stage which caused by the use of orthodontic appliances. As a conclusion of this study, the use of fixed orthodontic appliances may cause changes in epithelial mucosa which form an adaptation process by increasing the number of progenitor cells marked by cytokeratin 19.
Co-Authors Anusha S Vilvarajah, Anusha S Dwi Mutia Ramdhini, Dwi Mutia Murnisari Dardjan, Murnisari Nina Djustiana