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Immunoexpression of cytokeratin 19 in oral swab from fixed orthodontic appliance users Ramdhini, Dwi Mutia; Widyaputra, S. Sunardhi; Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 19, No 3 (2007): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2169.405 KB) | DOI: 10.24198/pjd.vol19no3.14156

Abstract

The use of fixed orthodontic appliances can improve someones mastication, speech and appearance. However, this appliance acts as a strange object that may cause irritation to the mucosa epithelial of oral cavity, because of the friction and pressure from the components of the fixed orthodontic appliances which are in direct contact with the oral mucosa. Irritation in the oral mucosa could stimulate the increase of cytokeratin. The appearance of cytokeratin is then used to identify the condition of these cells. This study was a descriptive research to find the expression of cytokeratin 19 with immunohistochemical method in oral mucosa epithelial of fixed orthodontic appliances users. Sample in this study was chosen from 30 fixed orthodontic appliances users. The result of this study was determined by calculating the number of positive cells (brown), compared with total number of cells. The account of positive cells would present the reaction of the epithelial cells according to the inflamation stage which caused by the use of orthodontic appliances. As a conclusion of this study, the use of fixed orthodontic appliances may cause changes in epithelial mucosa which form an adaptation process by increasing the number of progenitor cells marked by cytokeratin 19.
Determination of ovulation in women using saliva ferning test Ersyari, Riska Mutia; Wihardja, Rosiliwati; Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 26, No 3 (2014): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.537 KB) | DOI: 10.24198/pjd.vol26no3.14011

Abstract

Every human being experiences growth and development, starting from childhood to adulthood. Women who have entered puberty will experience monthly menstrual cycle. One phase of the menstrual cycle is ovulation or the fertile phase of a woman. The fertile period is the period in which there is an egg ready to be fertilized by sperm. At the time of fertility, there is an increase in the amount of estrogen and progesterone hormones. Increase in these hormones is also found in saliva. Saliva as a biological fluid in the body can be used as a diagnostic fluid. Woman’s fertile period can be assessed from the saliva. Saliva containing high estrogen hormones can form a ferning picture on saliva dried on object glass. The type of research is the study of literature. A literature study was conducted to discuss the determining of the fertile woman with saliva ferning test. The results of previous studies showed the existence of differences in saliva pictures at the time of the fertile period and the infertile period. Salivary ferning was very clearly seen in the woman’s fertile period.
Immunoexpression of cytokeratin 19 in oral cavity mucous smear of filter cigarette smokers Meira, Isra; Widyaputra, Sunardhi; Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 20, No 2 (2008): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.539 KB) | DOI: 10.24198/pjd.vol20no2.14138

Abstract

Smoking has become general habits in social life. One popular kind is filtered cigarette. As the base component is tobacco without clove and separated by the filter on it. Long irritation from heat and a toxic component of cigarette changed in oral mucosa epithelial. This condition can stimulate the increase of progenitor cells, which is marked by immunohistochemistry staining method of cytokeratin 19. This descriptive study is to find the expression of cytokeratin 19 in oral mucosa cytoplasm epithelial of filter cigarette smokers. There were 30 smokers which were selected with certain criteria. Samples were taken from a cytological smear of mucosa epithelial then stained with immunohistochemistry method. Analysis has taken by calculating the number of cells in the cytological smear. Then the immunoexpression of cytokeratin 19 was known from the percentage of cytoplasm cells which have brown colour compared with a total number of cells. In conclusion, the smoking filter cigarette would increase the number of progenitor cells from chronic inflammation, which is marked by cytokeratin 19 expression in oral mucosa cytoplasm epithelial of smokers.
Prevalence of oral cancer patients at the Pathology Anatomy Department RSUD Tasikmalaya year 2002-2007 Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 19, No 2 (2007): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.989 KB) | DOI: 10.24198/pjd.vol19no2.14183

Abstract

Malignancy was included which happened in the oral cavity. Oral cavity cancer is statistically fever than other parts of a body but can to be dead. The incidence of malignancy was increased in the last 50 years throughout the world. The purpose of this research is to get distribution data about oral malignancy cases frequency at Rumah Sakit Umum Daerah/RSUD Tasikmalaya. Rumah Sakit Umum Daerah Tasikmalaya is a Referal Hospital from East Priangan region, West Java Indonesia. This is retrospective descriptive research. Samples were taken from Histopathologic form data patient at RSUD Tasikmalaya from January 2002-December 2007. The result of this study indicated that 15 cases oral malignancy cases from all samples 2943 histopathology specimen in the period. The most malignancy cases of carcinoma were squamous cell carcinoma (98%). Tongue became the most area in this cases which was 46.66%. Oral malignancy were common in men 54%. The group of ages 60-70% became the majority namely 40%. The conclusion of the research indicated that carcinoma cell squamous were became the greatest part of oral malignancy types.
The effect of apple (Malus Domestica) juice on the damage of mice liver cells due to paracetamol treatment Hartanto, Anthony; Dardjan, Murnisari; Kintawati, Silvi
Padjadjaran Journal of Dentistry Vol 21, No 2 (2009): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.675 KB) | DOI: 10.24198/pjd.vol21no2.14095

Abstract

The liver is an important organ for body metabolism process. Liver disease is one of serious health problems in developing countries including Indonesia. Liver damage is caused by viral infection, toxic agent exposure (medications, alcohol), hormonal disturbance, neoplasm and autoimmune diseases. The use of high dose paracetamol to reduce pain also leads to liver damage. Apple (Malus domestica) juice is a natural anti oxidant agent. This laboratory experimental study was performed to discover the effect of giving apple juice on damaged cell regeneration due to the use of paracetamol. The study was performed in 21 male mice from Swiss-Webster strain that were divided into group I, II, and III. Group, I served as control while group II received 1 mg/ml paracetamol dose for 5 days and Group III received 1 mg/ml paracetamol for 5 days and 1 ml of apple juice on the 5th to 10th day. The observation of the mice liver cells was conducted using a light microscope with 400x magnification to get the number of necrotic liver cells per view field. The results of this study showed a difference in the number of necrotic liver cells between Group II and III. ANOVA statistical test ( = 0.05) concluded that apple juice significantly helps regeneration process in damaged liver cells caused by paracetamol.
Manajemen instalasi forensik di rumah sakit POLRI sebagai acuan pembentukan instalasi forensik kedokteran gigi di Rumah Sakit Gigi dan MulutManagement of forensic installations at POLRI Hospital as a reference for the establishment of forensic odontology installation at the Oral Hospital Munandar, Firstady Widyarnan; Oscandar, Fahmi; Malinda, Yuti; Dardjan, Murnisari
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 28, No 3 (2016): Desember
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.328 KB) | DOI: 10.24198/jkg.v28i3.18669

Abstract

Pendahuluan: Instalasi Forensik seharusnya terdapat bukan hanya di rumah sakit umum, tetapi juga di Rumah Sakit Gigi dan Mulut untuk menindaklanjuti kasus yang membutuhkan ilmu forensik kedokteran gigi. Tujuan penelitian adalah mempelajari gambaran unsur manajemen instalasi forensik di Rumah Sakit Kepolisian Pusat R.S Sukanto, Rumah Sakit Bhayangkara Sartika Asih serta Laboratorium dan Klinik Odontologi Kepolisian sebagai acuan pembentukan Instalasi Forensik Kedokteran Gigi di RSGM. Metode: Penelitian ini lakukan secara deskriptif dengan metode observasi. Lokasi penelitian di Rumah Sakit Kepolisian Pusat R.S Sukanto, Rumah Sakit Bhayangkara Sartika Asih, Laboratorium dan Klinik Odontologi Kepolisian. Hasil: Dari hasil penelitian menunjukan Instalasi Forensik Rumah Sakit Kepolisian Pusat Tk. I R.S Sukanto, Rumah Sakit Bhayangkara Sartika Asih serta Laboratorium dan Klinik Odontologi Kepolisian memiliki banyak kesamaan dari aspek unsur manajemen. Simpulan: Semua Instalasi Forensik yaitu Rumah Sakit Kepolisian Pusat R.S Sukanto, Instalasi Forensik Rumah Sakit Bhayangkara Sartika Asih serta Instalasi Forensik Odontologi Laboratorium dan Klinik Odontologi Kepolisian memiliki banyak kesamaan dari segi unsur manajemen dan dapat menjadi acuan untuk pembentukan unsur manajemen instalasi forensik kedokteran gigi di RSGM. ABSTRACTIntroduction: Forensic installations should be available not only in public hospitals, but also at the Dental and Oral Hospital to follow up on cases that require dental forensic science. The purpose of the study was to study the description of elements of forensic installation management at the Sukanto Hospital, Central Police, Bhayangkara Sartika Asih Hospital and the Police Odontology Laboratory and Clinic as a reference for the establishment of a Dentistry Forensic Installation at RSGM. Methods: This research is done descriptively by observation method. Research locations at Sukanto Hospital, Central Police Department, Bhayangkara Sartika Asih Hospital, Police Odontology Laboratory and Clinic. Results: From the results of the study showed the Forensic Installation of the Central Police Hospital. I R. Sukanto, Bhayangkara Sartika Asih Hospital and the Police Odontology Laboratory and Clinic have a lot in common with aspects of management. Conclusion: All Forensic Installations, Sukanto Hospital Central Police Hospital, Bhayangkara Sartika Asih Hospital Forensic Installation and the Odontology Laboratory Odontology Forensic Installation and Police have many similarities in terms of management elements and can be used as a reference for the management of dentistry forensic installations at RSGM .Keywords: Management elements, forensic installation, odomtology forensic installation.
Human age estimation based on pulp volume of canines for chronological age estimation: Preliminary research Hidayat, Septian Rahmat; Oscandar, Fahmi; Malinda, Yuti; Sasmita, Inne Suherna; Dardjan, Murnisari; Murniati, Nani; Lita, Yurika Ambar
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.681 KB) | DOI: 10.24198/pjd.vol30no3.19302

Abstract

Introduction: Age estimation has an important role in the process of human identification and needs to be performed in an efficient, fast, and accurate ways. Age estimation based on the pulp cavity volume of canines was referring to the theory stated that the apposition of secondary dentine during human life could be determined as an age indicator. This study was aimed to estimate the human age based on the pulp volume of canines for chronological estimation. Methods: Forty-one canine teeth from Cone Beam Computed Tomography (CBCT) 3D image sample from Dental Hospital of the Faculty of Dentistry Universitas Padjadjaran segmented into dental pulp and enamel were prepared for measurement of pulp volume using the ITK SNAP volumetric software. Descriptive analysis of pulp volume and regression equations were generated from regression analysis of pulp volume and tested for age estimation. Result: Dentine thickness was found to be increased from adolescence age range until adulthood age range. Logarithmic regression between pulp volume was statistically significant (p < 0,001). The coefficient of determination (R2) was 0.753 with a standard deviation of 8.421. Mathematics model for estimating age was: Age = 79.523 – (18.194 x ln(Pulp Volume)). The pulp cavity and dentine thickness were decreasing along the age. The same equation was also used in the same sample. There were several differences between chronological age and estimated age approximately 8.4 years following the regression result of R2 (75.3%). The rest of the sample (24.7%) have a big difference, and this could be affected by modifying variables such as occlusal force and human error measurement procedures. Conclusion: The human age can be estimated by the pulp volume of canines.Keywords: Age estimation, pulp volume, canine, ITK-SNAP, CBCT.
Microscopic appearance of healthy teeth, burned-tooth and automobile battery solution soaked tooth for the importance of visum in forensic dentistry Rozano, Randy; Dardjan, Murnisari; Oscandar, Fahmi
Padjadjaran Journal of Dentistry Vol 25, No 3 (2013): November 2013
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol25no3.26811

Abstract

Introduction: The hard tissue of the teeth is resistant to heat and acid,  so that the teeth have an important role as a material consideration of forensic dentistry. The humans have the same shape of the teeth around one in two billion. The stimuli of burn with high temperature and sour can be change the picture of macroscopic and microscopic tooth. This is the subject of an interesting comparison in the interests of justice and identification of dentistry. Methods: This research was a quasi experiment to analyzed change of microscopic teeth on the hard cases tooth-burning and the case hard acid treatment.The sample used were seven teeth, with details of one normal as a comparison, three teeth burned and three teeth soaked in a solution of water of accu.. Observations were made using the microscope at 40x enlarged on each tooth. The tooth was documented by means of a photographed and compared with healthy teeth. Observing and comparing the microscopic structure and the obtained differences in the teeth and treated with the picture of normal teeth. Results: The results of this study was obtained by observing and comparing the microscopic structure and the obtaineddifferences in the teeth and treated with the picture of normal teeth. Conclusion: The tooth are burned was visible loss of enamel at the cement-enamel junction as a result of the process of carbonization heat exposure so as to cause a breach of micro on the outer layer of the tooth enamel and thinning of dentin tubules, while the teeth are in the soak water accu (strong acid) seen change in color of enamel, micro cracks due to defective enamel and depletion of tubulus dentine and loss of boundary between the cementum and dentine.
Chemical composition of Citrus spp. and oral antimicrobial effect of Citrus spp. peels essential oils against Streptococcus mutans Aripin, Dudi; Julaeha, Euis; Dardjan, Murnisari; Cahyanto, Arief
Padjadjaran Journal of Dentistry Vol 27, No 1 (2015): March 2015
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol27no1.26751

Abstract

Introduction: The essential oils of orange peels had a considerable potential to be used as an antimicrobial agent. The aim of this present study is to analized chemical composition of Citrus spp. And the oral antimicrobial effect of Citrus spp. peels essential oils against Streptococcus mutans. Methods: Five orange peels species were used in this study consist of Lime (Citrus aurantifolia), Tangerine (Citrus nobilis), Sweet Orange (Citrus sinensis), Lemon (Citrus limon), and Kaffir Lime (Citrus hystrix). The isolated essential oils were analyzed using gas chromatography-mass spectroscopy (GC-MS). The Streptococcus mutans ATCC 25175 was employed against the antimicrobial effect of samples. Results: The extraction yields of hydrodistilled-essential oils from Tangerine, Kaffir Lime, Sweet Orange, Lemon, and Lime provided the extraction yields of 4.20, 2.26, 1.97, 1.74 and 0.83% yields, respectively. Major component essential oils of Citrus spp. was D-Limonene. The highest antimicrobial activity against S. mutans was Lime peel essential oil, followed by Lemon, Kaffir Lime, and Sweet Orange or Tangerine. All samples showed antimicrobial activity against S. mutans with the variation of antimicrobial action depending on the constituent of D-Limonene, β-Pinene, and α-terpineol. Conclusion: Major component chemical composition of essential oils of Citrus spp. was D-Limonene and antimicrobial activity by Lime peel essential oil due to its proportional amount of D-limonene and β-pinene and the highest antimicrobial activity.
Determination of ovulation in women using saliva ferning test Ersyari, Riska Mutia; Wihardja, Rosiliwati; Dardjan, Murnisari
Padjadjaran Journal of Dentistry Vol 26, No 3 (2014): November 2014
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.537 KB) | DOI: 10.24198/pjd.vol26no3.14011

Abstract

Every human being experiences growth and development, starting from childhood to adulthood. Women who have entered puberty will experience monthly menstrual cycle. One phase of the menstrual cycle is ovulation or the fertile phase of a woman. The fertile period is the period in which there is an egg ready to be fertilized by sperm. At the time of fertility, there is an increase in the amount of estrogen and progesterone hormones. Increase in these hormones is also found in saliva. Saliva as a biological fluid in the body can be used as a diagnostic fluid. Woman’s fertile period can be assessed from the saliva. Saliva containing high estrogen hormones can form a ferning picture on saliva dried on object glass. The type of research is the study of literature. A literature study was conducted to discuss the determining of the fertile woman with saliva ferning test. The results of previous studies showed the existence of differences in saliva pictures at the time of the fertile period and the infertile period. Salivary ferning was very clearly seen in the woman’s fertile period.