Ariyati Retno Pratiwi, Ariyati Retno
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Peran Decellurarized Dental Pulp Sebagai Kandidat Biomaterial Baru Dalam Regenerasi Pulpa : Tinjauan Literatur Pratiwi, Ariyati Retno; Salsabila, Dinia Fitri
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 21 No 1 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v21i1.47327

Abstract

Pulp necrosis is one of the main diseases that are most often found in the teeth and mouth. Root canal treatment is the main solution for pulp necrosis, but its implementation can lead to tooth death and recurrent infections. Regenerating the pulp by transplanting scaffolds, stem cells, and growth factors into the root canal is an alternative that can be offered to treat pulpal disease by keeping the teeth vital. Scaffold plays an important role in pulp regeneration, one of which is the ECM scaffold derived from pulp which combines the micro-collagen structure along with bioactive molecules that are important for pulp regeneration and able to promote the differentiation and proliferation of stem cells. This literature review aimed to identify the role of decellularized dental pulp as a new biomaterial candidate in pulp regenerations. The method being used in this review is systematic literature review using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) method. Decellularized Dental Pulp as a New Biomaterial Candidate has a role in pulp regeneration.
The Antibacterial Activity of Chitosan from Haruan (Channa striata) Fish Scales on the Growth of Streptococcus sanguinis Hutami, Wijayanti Diah Wasi; Tri Putri, Deby Kania; Carabelly, Amy Nindia; Kriswandini, Indah Listiana; Pratiwi, Ariyati Retno; Luthfi, Muhammad
Journal of Indonesian Dental Association Vol 3 No 2 (2020): October
Publisher : Indonesian Dental Association

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Abstract

Introduction: Recurrent Aphthous Stomatitis (RAS) is the most common oral lesion in Banjarmasin, with a prevalence of 45.42%. Streptococcus sanguinis (S. sanguinis) is thought to be one of the factors causing RAS. One natural ingredient that is often used by the people of South Kalimantan is the Haruan fish. The high Haruan fish consumption will eventually leave fish scales that have the potential to pollute the environment if not addressed immediately. Fish scales contain chitin, which when deacetylated produces chitosan, which has antibacterial properties. Objectives: The purpose of this study was to determine the effectiveness of the chitosan from the scales of the Haruan fish (Channa striata) at inhibiting S. sanguinis growth. Methods: This study used a randomized pretest-posttest with control group design using five treatments. The five treatments were subjected to liquid dilution using the UV-Vis Spectrophotometer method to obtain minimum inhibitory concentration (MIC) and a solid dilution test using the Total Plate Count method to obtain minimum bactericidal concentration (MBC). Results: The results showed that Haruan scale chitosan proved to be effective as an antibacterial against S. sanguinis, with a MIC of 1.25% and a minimum lethal concentration of 2.5%. One-Way Anova test results showed significance for the MIC test (p = 0,000) and MBC test (p = 0.000; p < 0.05). Conclusion: Chitosan from Haruan fish scales is both inhibitory and lethal to S. sanguinis.
Potensi Mengembang Hidrogel Komposit Chitosan dengan Tulang Sapi Demineralisasi Beku Kering untuk Regenerasi Tulang Wardani, Sinta Candra; Pratiwi, Ariyati Retno; Istikharoh, Feni; Pramaiswari, Made Adelia; Margareta, Isti Tri Aulia
E-Prodenta Journal of Dentistry Vol 10 No 1 (2026):
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2026.009.01.2

Abstract

Backgrounds: Demineralized freeze-dried bovine bone synthesized in nanoparticle size is expected to improve the osteoinductive of chitosan hydrogel as bone regeneration material. Therefore, an evaluation was carried out on whether this material can affect swelling as one of the physical properties of the hydrogel. Objective: To determine the swelling potential test of the hydrogel composite with varying concentrations of Demineralized Freeze-Dried Bovine Bone Nanoparticle (DFDBB-Np). Methods: The chitosan hydrogels at DFDBB-Np concentrations of 0%, 0.5%, 1%, 1.5%, 2%, and 2.5% are incubated in sterile water at 37°C for varying durations (3, 6, 12, 24 hours) until swelling occurs. The equilibrium Swelling Ratio (SR) is calculated as the ratio of the swelling to the initial weight. Results: After 24 hours of incubation, a significant difference was observed between concentrations of 0% and 0.5%, 1%, 1.5%, 2%, and 2.5% (sig = 0.015, 0.004, 0.000, 0.001, 0.000). However, other concentrations and duration did not show any significant difference. Conclusion: DFDBB-Np retains its potential as a composite material with chitosan hydrogel for bone regeneration.
Efek Pemberian Nanoemulgel Ekstrak Moringa oleifera terhadap Jumlah Sel Makrofag Rattus norvegicus Pratiwi, Ariyati Retno; Risnauli, Yanice Natalia Regar
E-Prodenta Journal of Dentistry Vol 10 No 1 (2026):
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2026.010.01.5

Abstract

Introduction: Inflammation is a complex response of the body's immune system that refers to pathological conditions against various harmful stimuli. One of the factors causing inflammation is the presence of ulcers. At the beginning of the inflammatory response, macrophages tend to be polarized into the M1 phenotype (pro-inflammatory) then switch to the M2 phenotype (anti-inflammatory). The balance between M1 and M2 macrophage activity is essential for proper inflammation and optimal tissue repair. In the context of macrophage modulation, nanoemulgel formulation has the potential to increase the delivery of bioactive compounds from Moringa oleifera extract to target tissues, facilitate interactions with tissue macrophages, and enhance anti-inflammatory and immunomodulatory effects. Objective: This study aims to determine the effect of administering Moringa oleifera extract nanoemulgel on the number of Rattus norvegicus macrophage cells. Method: This study used a true experimental in vivo with a Randomized Post Test Only Control Group design using 24 male Wistar rats divided into 4 groups, 2 control groups and 2 treatment groups. The variables studied were the number of macrophage cells observed on the 5th day. Results: The results showed that Moringa oleifera extract nanoemulgel had an effect on the number of M1 Rattus norvegicus macrophage cells. This was evidenced by a significant increase in the number of M1 macrophage cells in the 30% Moringa oleifera extract nanoemulgel treatment group on the 5th day. Conclusion: After administering Moringa oleifera extract nanoemulgel, there was a difference in the number of macrophage cells in the inflammatory process observed on the 5th day.