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Gleni Hasan Huwoyon, Gleni Hasan

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Author-ID : 597780

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Other

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PENGARUH PEMBERIAN HORMON METHYLTESTOSTERONE PADA LARVA IKAN GUPPY (Poecilia reticulata) TERHADAP PERUBAHAN JENIS KELAMIN Huwoyon, Gleni Hasan; -, Rustidja -; Gustiano, Rudhy -
ZOO INDONESIA Vol 17, No 2 (2008): November 2008
Publisher : Masyarakat Zoologi Indonesia

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Huwoyon, G.H., Rustidja & R. Gustiano. 2008. Effect of methyltestosterone hormoneon sex reversal of Guppy (Poecillia reticulate) larvae fish. Zoo Indonesia 17(2): 49-54. Male ornamental fish is more exotic than female one. The objective of study wasto increase the percentage of male guppy fish (Poecillia reticulate) usingmethyltestosterone hormone at the larvae stage. Control (A = 0 ppm) and four differentdosages of methyltestosterone hormone (B = 5 ppm, C = 10 ppm, D = 15 ppm & E =20 ppm) were used as treatments with three replications. The results showed the besttreatment to produce the highest percentage (58.41%) of male guppy fish was at 10ppm dosage (p<0.01). However, the dosages hormone treatments given in this studywere not significantly different on intersexes production, survival rate and specificgrowth rate (p>0.05).

RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements] Iskandariah, Iskandariah; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Kusmini, Irin Iriana; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.

ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)] Gustiano, Rudhy; Oktaviani, Tia; Soelistyowati, Dinar Tri; Kusmini, Irin Iriana; Wahyutomo, Wahyutomo; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and âTrussâ morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.

OPTIMALISASI KEPADATAN BENIH IKAN MAS (Cyprinus carpio) STRAIN RAJADANU PADA PENDEDERAN DI KOLAM AIR TENANG [Density Optimization of Carp Seed (Cyprinus carpio) Strain Rajadanu in the Nursery in Calm Pool Water] Radona, Deni; Asih, Sidi; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

National fisheries production is not proportional to the increase of the population now, so that the consumption of animal protein needs are not met.For the sake of national fisheries production there is a need for intensive cultivation technology breakthroughs to spur the density in nursery phase ârajadanuâ carp (Cyprinus carpio) strain. This experiment aim to determine the appropriate density of rajadanu carp growth in the pond at the nursery phase. The average length of tested fishes is 2.02 cm and the average initial weight is 0.25 g maintained in the out door plot pool of Research Installation for Germ Plasm of the Research and Development Division of Freshwater Fisheries, Cijeruk, Bogor measuring 1x1x1 m with density 100, 150 and 200 fishes/m3 .Each treatment was repeated 3 times. Feed used was a commercial feed containing 28% protein given in 2 times a day as much as 5% of the total weight of fish. The results showed that after 40 days, each treatment showed no significant difference (P> 0.05) with absolute length and weight growth of the absolute highest in nursery density of 200 fishes/m3 of 1.30 Â± 0.10 cm and 1.14 Â± 0, 18 cm with a daily growth rate of 1.30 Â± 0.10% and 4.08 Â± 0.55%, and the highest survival at densities 200 fishes/m3 of 92.5 Â± 6.55%.

PEMBESARAN IKAN BAUNG {Hemibagrus nemurus) YANG DIBERI PAKAN BERBEDA DIKOLAM TANAH Huwoyon, Gleni Hasan; Suhenda, Ningrum; Nugraha, Aditiya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Green catfish is a commodity that is not only potential to be cultivated in the pond and in the floating net cages, but also easy to adjust to the artificial feed. The objective of this research is to evaluate the effect of different feed types (sinking and floating)on the growth performance of green catfish(Hemibagrus nemurus.One thousand and six hundred fingerlings averaging 2.50 g of 3 individual body weight were stocked in each of earthern ponds (10 x 8 x 1.2 m ). They were fed daily for four months with the same protein content (31%) and lipid (6%) diets. The feed was given in pelleted form at 8-4% of the total body weight. The result of this study showed that no significant difference (P>0.05) between different feed types. The sinking feed gave specific growth rate (2.35%), feed conversion ratio (2.49), protein retention (18.92%) and protein efficiency ratio 1.13 while the floating type gave the values 2.49%, 2.37, 20.48% and 1.21, respectively. Lipid retention of sinking feed (34.84%) is lower than that of floating type(48.39%).The survival rates ranged between 76.56-84.54%.Feed type sinking with protein content of 31% can be used in the rearing of fish in earthern pond.

Co-Authors Asih, Sidi Deni Radona, Deni Dinar Tri Soelistyowati Irin Iriana Kusmini, Irin Iriana Iskandariah, Iskandariah Ningrum Suhenda Nugraha, Aditiya Oktaviani, Tia Rudhy - Gustiano, Rudhy - Rudhy Gustiano Rustidja - -, Rustidja - Wahyutomo, Wahyutomo

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