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NILAI HETEROSIS DAN PERANAN INDUK PADA KARAKTER PERTUMBUHAN HASIL PERSILANGAN INTERSPESIFIK Tor soro DAN Tor douronensis [Growth Heterosis Values and The Role of Parent Tor soro and Tor douronensis in Interspesific Crossed] Radona, Deni; Subagja, Jojo; Kusmini, Irin Iriana; Gustiano, Rudhy
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2784.425 KB) | DOI: 10.14203/beritabiologi.v15i2.2394

Abstract

Fish Tor is one of potential fish commodities to be developed because of their economic value. This on experiment was designed to evaluate the effect of male and female parent on morphological characters i.e length and weight growth and the value of heterosis from crosses of Tor soro and Tor douronensis. Crosses were performed in both directions (reciprocal) to form four populations. Observations lenght, weight gain, specific growth in length, specific growth in weight and value of heterosis were carried out for two months (January-March, 2014). The results showed an increase of  length, weight, specific growth in weight, specific growth in length occurred in seed produced from parent male of T. soro and female of T. douronensis with values of 0.54 cm, 0.09 g, 0.65 % and 3.79 %, rescpectively. Crossed of ? T. douronensis x ? T. soro yield heterosis value (partial) of length (40.90%) and weight (116.66%) was higher than crossed of ? T. soro x ? T. douronensis  that only have heterosis value of 0 and 33.33 %, and heterosis value (reciprocal) on the length of 20.45 % and the weight of 45.22 %.
RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements] Iskandariah, Iskandariah; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Kusmini, Irin Iriana; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.892 KB) | DOI: 10.14203/beritabiologi.v14i1.1866

Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.
ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)] Gustiano, Rudhy; Oktaviani, Tia; Soelistyowati, Dinar Tri; Kusmini, Irin Iriana; Wahyutomo, Wahyutomo; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.515 KB) | DOI: 10.14203/bb.v12i3.641

Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.
KARAKTERISASI GENETIK IKAN KELABAU (Osteochilus kelabau) DARI BERBAGAI LOKASI DI KALIMANTAN BARAT MENGGUNAKAN METODE RAPD (Random Amplified Polymorphism DNA) Kusmini, Irin Iriana; Gustiano, Rudy; Mulyasari, Mulyasari
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.725 KB) | DOI: 10.14203/beritabiologi.v10i4.762

Abstract

Kelabau fish (Osteochilus sp.) is an endemic fish to Kalimantan inland waters that is potential to be developed. The aim of this research was to characterize Kelabau fish and to study the data base (genetic character) of kelabau as well as its relationship. The result showed that the highest polymorphism and heterozigosity was on Kelabau Sintang and the lowest was on Kelabau Kapuas Hulu. The closest genetic distance value were between Kelabau Pontianak-Kapuas Hulu (0.5351) and the furthest were between Kelabau Pontianak-Sintang (0.6852).
HUBUNGAN PANJANG DAN BERAT, FAKTOR KONDISI , FEKUNDITAS, DAN PERKEMBANGAN TELUR IKAN TENGADAK (Barbonymus schwanenfeldii) DARI SAROLANGUN, JAMBI DAN ANJONGAN, KALIMANTAN BARAT, INDONESIA Kusmini, Irin Iriana; Subagja, Jojo; Putri, Fera Permata
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4785.944 KB) | DOI: 10.14203/beritabiologi.v17i2.3017

Abstract

Tinfoil barb (Barbonymus schwanenfeldii) is one of the potential local fish to be cultivated as a food or pet fish. The data and information of the growth pattern of species are essential for fish farming success. The observation on the length and weight relationship is an usefull indicator to determine the pattern of growth fish observed. This study aims to determine the growth patterns of tinfoil barb fish based on length and weight relationship, condition factor and fish fecundity of tinfoil barb from Sarolangun, Jambi and Anjongan, West Kalimantan. The 30 samples were taken randomly from each group. Data were analyzed using analysis regression Microsoft Excel. The result showed that the regression coefficient of length and weight relationship was 2.811 for Sarolangun and 2.686 for Anjongan. The regression value indicates that the the growth of tinfoil barb was allometric negative, with an average factor condition ranged from 0.99 to 1.002. Length and weight relationship had determinant value (R2) ranged from 0.79 to 0.96, with the fecundity ranged from 20168 to 232.040 eggs from 9–45.5g of gonad weight. 
HUBUNGAN PANJANG DAN BOBOT IKAN NILA LOKAL, BEST F5 DAN F6 DI PANGKEP, SULAWESI SELATAN PADA UMUR 60 HARI PEMELIHARAAN Kusmini, Irin Iriana; Gustiano, Rudhy; Putri, Fera Permata
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.685

Abstract

Best tilapia is breed fish developed by Research Center and Development of Freshwater Aquaculture (BPPBAT) Bogor that has been launched since 2008. Best tilapia is the third generation of gift tilapia. In order to obtain a better growth performance, BPPBAT continued the process of breeding, and to date we have obtained the sixth generations of Best tilapia. This study was aimed to determine growth of tilapia through observation of the relationship between the length and weight of local tilapia, F5 and F6 BEST which were maintained in brackish waters in the Pangkep area, South Sulawesi. Fish samples were taken at random, 30 fishes were taken from each population for length and weight measurement. Calculation of the length-weight of fish in the Pangkep area obtained regression coefficient (b) for local fish 2.4361; BEST F5 3.1077 and F6 3.0522. These values indicated that the local tilapia was negatively allometric while BEST was positively allometric, with local fish condition factor ranged between 0.471-1.334, F5; 0.468-1.479 and 0.498-1.534 F6 respectively. This results has indicating the normal condition of the fish. The length-weight relationship of fish had a value of determinant (R 2) ranged from 0.7829-0.9182, and indicated fish variations influenced by other variables was rather small and has suggested close relationship between length and weight.
A STUDY ON THE GROWTH AND PHYSIOLOGICAL RESPONSE OF JUVENILE TINFOIL BARB Barbonymus schwanenfeldii (Bleeker, 1854) UNDER THE INFLUENCE OF pH CHANGES Oktavia, Nia; Prakoso, Vitas Atmadi; Kamal, Mohammad Mukhlis; Arifin, Otong Zenal; Subagja, Jojo; Kurniawan, Kurniawan; Iswantari, Aliati; Kusmini, Irin Iriana; Irawan, Deni; Cahyanti, Wahyulia; Radona, Deni; Putri, Fera Permata; Bosman, Ofan; Wibowo, Arif; Kristanto, Anang Hari; Gustiano, Rudhy
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.35-44

Abstract

Tinfoil barb Barbonymus schwanenfeldii is recognized as one of the potential aquaculture commodities in Indonesia. Nonetheless, lack of data on their environmental tolerance causing this issue to become essential to be investigated. The present study was carried out to assess the pH influence on growth and physiological response of juvenile tinfoil barb. Juvenile tinfoil barbs (TL: 5.5 ± 0.8 cm; BW: 2.4 ± 1.0 g) were treated with four pH level treatments (5, 6, 7, and 8) and three replications with the stocking density of 10 fish each aquarium. Fish were kept for 21 days and fed with commercial fish feed. In this study, pH 8 exposure resulted to lower growth of juvenile tinfoil barb compared to pH treatment 5 to 7 (p<0.05). In terms of survival rate, the results show significantly lower value at pH 8 treatment compared to those of pH 6 and 7 treatment (p<0.05), while it is not significantly different with pH 5 treatment (p>0.05). Additionally, this study found the highest value of glucose, cortisol, and aspartate aminotransferase (AST) on pH 8 treatment (p<0.05). On the other hand, pH 8 exposure led to the lowest creatinine, blood urea nitrogen (BUN), and ALP level (p<0.05). In conclusion, the optimal pH for juvenile tinfoil barb rearing ranged around 6-7.
FIRST GENERATION REPRODUCTIVE PERFORMANCE AND SECOND GENERATION LARVAL PRODUCTION ON THE DOMESTICATED TINFOIL BARB, Barbonymus schwanenfeldii (BLEEKER, 1854) Kusmini, Irin Iriana; Cahyanti, Wahyulia; Gustiano, Rudhy; Sundari, Sri; Iskandar, Andri; Radona, Deni; Kurniawan, Kurniawan; Prakoso, Vitas Atmadi; Putri, Fera Permata; Arifin, Otong Zenal; Subagja, Jojo; Kristanto, Anang Hari; Prihadi, Tri Heru; Mulyasari, Mulyasari; Yosmaniar, Yosmaniar; Subaryono, Subaryono
Indonesian Aquaculture Journal Vol 18, No 2 (2023): (December, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.2.2023.97-104

Abstract

Tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854), is a potential freshwater fish for Indonesian aquaculture. Before widely used, the candidate needs to be evaluated. This research aimed to evaluate the reproductive performance of the first generation (G1) and larval performance of the second generation (G2) of domesticated tinfoil barb. A total of 68 female and 24 male broodstocks were selected for the artificial breeding program. The gonadal maturity test was carried out by canulating the eggs every month. Before spawning, GnRHa hormone was injected into the dorsal area (0.5 mL/kg for female and 0.2 mL/kg for male). Fecundity, fertility rate, hatching rate, embryogenesis, and larvae ontogeny were recorded. The results showed that the first matured G1 males of tinfoil barb were at the standard length of 16.01 ± 1.18 cm, while females at 15.79 ± 1.23 cm. The mature broodstock indicated by the gonad maturity stage III and IV confirming higher estradiol concentration (above 400 pg mL-1). The fecundity of two mature broodstock-sized of 217.2 g and 197.3 g were 12,495 and 15,782 eggs, respectively. The spawning season of G1 tinfoil barb was in October and November (rainy seasons). The fertilized eggs latency time was 10 hours 44 minutes at 25°C and hatched after 23 hours 7 minutes. The fertility rate was 96.96 % and the hatching rate was 95.16 %. The survival rate of G2 normal larvae was 100 % at three days of the rearing period. The G2 larvae production in this experiment provides an excellent opportunity for fish diversification both for aquaculture and restocking.
RESILIENCE AND PHYSIOLOGICAL RESPONSES OF THE DOMESTICATED ASIAN REDTAIL CATFISH Hemibagrus nemurus TO HYPOXIA CONDITION Iswantari, Aliati; Kurniawan, Kurniawan; Prakoso, Vitas Atmadi; Radona, Deni; Irawan, Deni; Putri, Fera Permata; Cahyanti, Wahyulia; Arifin, Otong Zenal; Subagja, Jojo; Gustiano, Rudhy; Kusmini, Irin Iriana; Wibowo, Arif; Kristanto, Anang Hari
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.53-60

Abstract

Hypoxia is one of the critical issues in aquaculture production systems as it can lead to physiological disturbances in cultured fish. This research aimed to evaluate the tolerance level and physiological responses of domesticated Asian redtail catfish Hemibagrus nemurus reared in various hypoxia conditions. A total of 12 fish/treatment were acclimated to gradually decreased dissolved oxygen treatments until fish experienced aquatic surface respiratory (ASR) and loss of equilibrium (LOE). Cortisol, haemoglobin, and glucose levels were detected in the blood plasma to evaluate the stress response of the fish to hypoxia. The result showed that ASR of H. nemurus was identified at 2.17 ± 0.14 ppm of dissolved oxygen (DO) concentration with the percentage of ASR was 77.67 ± 9.53%, while LOE critical of H. nemurus happened at 0.63 ± 0.15 ppm of DO where 55.56 ± 4.81% of the fish experienced LOE. There were significant differences in the values of physiological parameters (blood cortisol, haemoglobin, and glucose) between control and treatments as fish experienced LOE (P<0.05). In the present  study, it was found that the Asian redtail catfish is classified as a hypoxia-sensitive fish group. Tehis finding is valuable information for the rearing and growing of the fish to provide an optimal DO concentration for their growth and survival.
Small Tropical Abalone (Haliotis squamata Reeve, 1846) in Indonesia: Current Research Status and Future Prospect Permana, I Gusti Ngurah; Gustiano, Rudhy; Chodriyah, Umi; Ahmad, Riza Zainuddin; Kusmini, Irin Iriana; Lukman, Lukman; Yosmaniar, Yosmaniar; Kadarini, Tutik; Kurniasih, Titin; Murdinah, Murdinah
Zuriat Vol 35, No 2 (2024): September, 2024
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v35i2.56861

Abstract

Abalone farming is gaining popularity worldwide as demand grows. The importance of abalone farming throughout Indonesia is due in part to expanding demand for both local and foreign abalone, and to a growing awareness of the necessity for sustainable seafood production. This study relies on fourty-six scientific papers published between 2000 and 2023. The distribution and variety, bio-reproduction and breeding, growth, and commerce of small tropical abalones are carefully explored to highlight the current research state, as well as the opportunities and challenges in the existing abalone sector. Finally, all interactions yield findings that can be utilized to guide government policy. To realize its full potential, the Indonesian abalone industry must overcome difficulties such as competition and price fluctuations. Key goals include developing sustainability collection and trading methods, reliably supplying high-quality abalone goods, and expanding their reach through global trade. Addressing these challenges will enable the Indonesian abalone industry to thrive in the next years. The abalone business in Indonesia has a long history, is now growing and active, and presents significant potential. With ongoing R&D investments, the Indonesian abalone sector can meet national and global market demands while being sustainable. Understanding the current situation of abalone research in Indonesia is critical to maintaining the industry's long-term survival.