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All Journal Bioeksperimen: Jurnal Penelitian Biologi Biology Natural Resources Journal
Nadia Puspita Maya
Universitas PGRI Ronggolawe
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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DETEKSI CEMARAN BAKTERI Eschericia Coli PADA DAGING AYAM BROILER DI UPT LABORATORIUM KESEHATAN HEWAN DINAS PETERNAKAN PROVINSI JAWA TIMUR DI TUBAN Nadia Puspita Maya; Kuntum Febriyantiningrum; Mahdi Selomashar; Nia Nurfitria
Binar – Biology Natural Resources Journal Vol 1 No 2 (2023)
Publisher : Prodi Biologi Universitas PGRI Ronggolawe

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.789 KB) | DOI: 10.55719/Binar.2023.2.1.6-12

Abstract

Food safety is the main demand of consumers. Food quality and safety must be ensured to be free from pathogenic materials and safe for consumption. One of the pathogenic bacteria found as contaminants in broiler chicken meat is the Escericia coli. Broiler meat products that are accepted and that will be consumed by the community, they must meet the specified requirements and be proven by a certificate of test results from a recognized institution and based on ISO 17025. One of the testing agencies that has been accredited by the Komite Akreditasi Nasional (KAN), namely the UPTD Laboratorium Kesehatan Hewan Dinas Peternakan Provinsi Jawa Timur Livestock Service, provides community services in laboratory examinations for testing microbial contamination in animal products (meat, milk and eggs) with quality reference standards for microbial contamination limits according to SNI 7388: 2009. The method used in this study is to use the MPN method. This study used 2 samples of broiler chicken meat from PT. Trijaya Lestari Food. The MNP method is a method used to determine the calculation and estimate the amount of contamination of Escherichia coli bacteria contained in broiler chicken meat samples. The result obtained from this study is that the number of positive tubes shows the MPN value as the amount of Escherichia coli per ml / per g. For dilutions 10ˉ¹, 10ˉ² and 10ˉ³ are valued at 0 and if added up the value is <3.0 which indicates that broiler chicken meat samples are below the maximum contamination limit of the number of Escerichia coli bacteria based on SNI 01-3924-2009.
Exploration of nyamplung rizosphere nitrogen fixing bacteria in mine reclamation Sriwulan Sriwulan; Tita Dewi Fitriani; Nadia Puspita Maya; Ni&#039;matur Rohmah; Eko Purnomo
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23011

Abstract

Nitrogen fixing bacteria have the ability to utilize free nitrogen as a nitrogen source for their growth. These bacteria are able to make efficient availability of N- in the soil, so that it can be utilized by plants. The aim of this study was to obtain nitrogen fixing bacteria isolates from the rhizosphere of nyamplung plants in limestone reclamation lands. Isolation and testing of nitrogen fixation ability by nitrogen fixing bacteria was carried out using selective Ashby's Mannitol Agar media. The isolation results obtained 5 isolates on Ashby's Mannitol Agar media. The morphological characters of the colonies of the five isolates showed round and irregular shapes, flat and convex elevations with flat edges, white, cloudy white and clear. Microscopic observation showed that the isolates consisted of gram-positive and negative bacteria with bacilli cell shape. The catalase test showed 4 positive isolates and 1 negative. The conclusion of this study was that 5 nitrogen fixing bacteria isolates were obtained from the rhizosphere of the nyamplung limestone land plant. Isolates 1, 3, and 5 showed the character of the genus Clostridium, isolate 2 led to the genus Bacillus, and isolate 4 had the same character as the genus Klabsiella.
Co-Authors Eko Purnomo Kuntum Febriyantiningrum Mahdi Selomashar Ni&#039;matur Rohmah Nia Nurfitria Sriwulan, Sriwulan Tita Dewi Fitriani