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All Journal Journal of Tropical Marine Science
Gregorius Nugroho Susanto
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Isolasi, Karakterisasi, dan Identifikasi Bakteri Endofit Sargassum polycystum Penghasil Enzim Alginat Lyase Yesica Bella Safitri; Agus Setiawan; Ni Luh Gede Ratna Juliasih; Endang Linirin Widiasuti; Gregorius Nugroho Susanto
Journal of Tropical Marine Science Vol 6 No 1 (2023): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v6i1.3732

Abstract

Alginate, polysaccharide from marine brown algae, is widely used for antitumor, anti-inflammatory, and also antiviral. However, alginate has large molecular weight that difficult to digest in some organsims. Therefore, it is important to look for the alginate lyase enzyme to break down the alginate. The purpose of this study was to obtain bacterial isolates that produce alginate lyase enzymes from Sargassum polycystum. Isolation of alginate lyase-producing bacteria was carried out by collecting Sargassum polycystum. from Sebalang Beach, Tarahan, South Lampung then the bacteria were isolated by the dilution method. The colonies obtained were purified and selected using qualitative (clear zone) and semi-quantitative activity tests, and identified morphologically, biochemically and molecularly using the 16S-rDNA gene. In this study, 20 pure bacterial isolates were obtained with one isolate having alginate lyase activity, namely PTF and PTH. The results of biochemical tests and molecular characterization showed that the isolate was Bacillus cereus.
Analisis Kualitas Air Sebagai Pemicu Eutrofikasi di Habitat Kerang Bulu (Anadara antiquata) di Pesisir Kabupaten Pesawaran Andy Sahalatua Gultom; Qadar Hasani; Gregorius Nugroho Susanto; Munti Sarida; Endang Linirin Widiastuti; Winarto Santosa
Journal of Tropical Marine Science Vol 9 No 1 (2026): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekvmbn43

Abstract

The blood cockle (Anadara antiquata) is an economically valuable marine species that depends on suitable water quality conditions in its habitat. This study aims to analyze water quality parameters that potentially trigger eutrophication in the habitat of the blood cockle in the coastal waters of Pesawaran Regency, Lampung Province. The study was conducted at three key harvesting sites—Teluk Hurun, Teluk Cikunyinyi, and Teluk Ratai—with sampling carried out four times during September–October 2024. The measured parameters included temperature, pH, salinity, dissolved oxygen (DO), transparency, depth, nitrite, ammonia, phosphate, and total organic matter (TOM). The results showed that several chemical parameters, such as nitrite (up to 0.11 mg/L), phosphate (up to 0.63 mg/L), and TOM (up to 39.14 mg/L), exceeded marine water quality standards based on Government Regulation No. 22 of 2021. The low N/P ratio (<1) at all sites indicates a high potential for eutrophication, with Teluk Ratai identified as the most critical site. These findings highlight the importance of continuous water quality monitoring to support the sustainability of blood cockle habitats and coastal ecosystems.
Co-Authors Agus Setiawan Andy Sahalatua Gultom Endang Linirin Widiastuti Endang Linirin Widiasuti Munti Sarida Ni Luh Gede Ratna Juliasih Qadar Hasani Winarto Santosa Yesica Bella Safitri