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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal EnviScience (Environment Science) Jurnal Ners
Pendrianto Pendrianto, Pendrianto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Health Professions Medicine & Pharmacology Nursing Public Health

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Potential of Soursop Leaf Extract as an Antioxidant in MCF-7 Cells Mustofa, Muhammad Samsul; Hadi, Restu Syamsul; Rahmah, Nunung Ainur; Pendrianto, Pendrianto
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.265-269

Abstract

The frequency of breast cancer tends to increase. Malondialdehyde (MDA) is a marker of oxidative stress as an end product from the chain reaction of lipid peroxidation. The use of traditional medicine soursopleaf (Annona muricata L.) has been reported for a long time because of its bioactivity as an antioxidant. This study analyzes the relationship between MDA levels and glutathione enzymes in MCF-7 cells given the methanol extract of soursop leaves. The methanol extract of soursop leaves was carried out by infusion method. The methanol extract of soursop leaves was given to cancer cells at several doses with an incubation of 24 hours. The cytotoxic test was carried out using the MTT method. Measurement of MDA levels was carried out using the thiobarbituric acid reactive substance (TBARS/TBA) reactivity test method. GSH measurements used the colorimetric method. The results showed that the ethanol extracts of soursop leaves have cytotoxic activity in the MCF-7 breast cancer cell line with IC50 values of 23.96 ppm. Ethanol extract of soursop leaves increased levels of MDA inhibition and GSH level. Soursop leaf extract could increase MDA inhibition GSH level in human breast cancer cells MCF-7.
Detection of Mice DNA in Meatballs Using Real Time – PCR Septiani, Triayu; Pendrianto, Pendrianto
Jurnal EnviScience (Environment Science) Vol. 2 No. 2 (2018)
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.591 KB) | DOI: 10.30736/jev.v2i2.66

Abstract

Meat is one of perishable food and has the high of water content and protein content. Meatballs are processed food who are using meat as main ingredient and other ingredients, such tapioca flour, salt, pepper, and the other ingredients as food additives. Meatballs is the mostpopular because has tasty flavor, high protein content, especiallyessential amino acids.In Indonesia, until now there were still cases of counterfeiting of processed meat products. One of method is mixing the beef meat and other animalsmeat in the making process to reduce production costs, so that similar products can be obtained at a much lower cost. One common case is the mixing of beef meat with rat meat. This study aims to identify meatballs contaminated by rat meat. Meatball samples used do not have Halal certificate from LPPOM MUI. From the results of the study, obtained the green amplification curve with the highest peak showed positive control of rat DNA, followed by three other green curves from the sample that showed the occurrence of amplification, thus indicating samples positive containing rat DNA. In the positive sample, the rat DNA concentration in the sample is quite low which can be seen from the intensity of the peak produced on the amplification curve when compared with positive control. Keywords :DNA, Meat,Meatballs, Rat, RT – PCR
Pengaruh Efek Ekstrak Kayu Bajakah Merah (Sphatolobus littoralis) Terhadap Kadar Protein mTOR pada Sel Kanker Payudara MCF-7 Siskowati, Ratu Salia; Harliansyah, Harliansyah; Rahmah, Nunung Ainur; Randy, Ahmad; Pendrianto, Pendrianto
Jurnal Ners Vol. 10 No. 1 (2026): JANUARI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i1.54258

Abstract

Abstrak Salah satu penyakit yang paling umum terjadi pada wanita adalah kanker payudara, yang terkait dengan pembelahan sel yang tidak terkendali melalui jalur sinyal mTORC. Studi ini bertujuan untuk menentukan bagaimana ekstrak Kayu Bajakah Merah (Spatholobus littoralis) mempengaruhi ekspresi protein mTORC secara in vitro pada sel kanker payudara MCF-7. Penelitian menggunakan desain true experimental dengan post-test only control group, melibatkan enam kelompok perlakuan, termasuk kontrol negatif, kontrol positif (Tamoxifen), dan perlakuan ekstrak Bajakah Merah pada konsentrasi IC₅ 72 jam. Sel dikultur, diolah, dan viabilitas sel diukur menggunakan CCK-8 untuk menentukan IC₅, sedangkan kadar protein mTORC dianalisis menggunakan ELISA. Hasil penelitian menunjukkan bahwa ekstrak Bajakah Merah menurunkan viabilitas sel secara dosis- dan waktu-dependen, dengan IC₅ terendah pada inkubasi 72 jam. Kadar mTORC meningkat signifikan pada kelompok perlakuan ekstrak dibanding kontrol negatif, menunjukkan pengaruh ekstrak terhadap jalur pensinyalan mTORC. Meskipun efek sitotoksik masih lebih rendah dibanding Tamoxifen, ekstrak kayu Bajakah Merah memiliki potensi sebagai agen kemopreventif berbasis bahan alam.. Kata Kunci: kanker payudara, MCF-7, ekstrak kayu Bajakah Merah, mTORC, sitotoksik, kemoprevensi. Abstract One of the most prevalent diseases in women is breast cancer, which is linked to unchecked cell division via the mTORC signaling pathway. This study sought to determine how Red Bajakah Wood (Spatholobus littoralis) extract affected the in vitro expression of the mTORC protein in MCF-7 breast cancer cells. A true experimental design with post-test only control group was employed, including six treatment groups: negative control, positive control (Tamoxifen), and Red Bajakah Wood extract at IC₅₀ 72-hour concentration. Cells were cultured, treated, and viability assessed using CCK-8 to determine IC₅₀, while mTORC protein levels were measured via ELISA. Results showed that Red Bajakah Wood extract reduced cell viability in a dose- and time-dependent manner, with the lowest IC₅₀ at 72 hours incubation. mTORC levels increased significantly in extract-treated cells compared to negative control, indicating the extract’s effect on the mTORC signaling pathway. Although its cytotoxicity was lower than Tamoxifen, Red Bajakah Wood extract demonstrated potential as a natural-based chemopreventive agent.. Keywords: breast cancer, MCF-7, Red Bajakah Wood extract, mTORC, cytotoxicity, chemoprevention.
Co-Authors Ahmad Randy Harliansyah, Harliansyah Mustofa, Muhammad Samsul Nunung Ainur Rahmah Restu Syamsul Hadi Siskowati, Ratu Salia Triayu Septiani, Triayu