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All Journal Jurnal Sain Peternakan Indonesia Jurnal Media Pertanian
Siti Shofiya Nasution
Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Languange, Linguistic, Communication & Media Veterinary

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Eksplorasi Bakteri Antagonis Asal Jaringan dan Rizosfer Tanaman Celosia argantea Untuk Menekan Pertumbuhan Fusarium sp. Secara In Vitro Siti Shofiya Nasution; Alfi Saryanah
Jurnal Media Pertanian Vol 8, No 1 (2023): April
Publisher : Universitas Batanghari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jagro.v8i1.176

Abstract

Fusarium sp. is a fungus causing serious disease in many plants. This study aims to explore antagonistic agents which potential to suppress Fusarium sp. in vitro. This research was conducted from October to December 2015 at Nematology laboratory, Department of Plant Protection, Bogor Agricultural University. The materials used in this study were Celosia argantea, 20% and 100% TSA media, sterile distilled water, 3% sodium hypochlorite (NaOCl). The tools used are laminar air fliw, autoclave, shaker, petridish. This candidate antagonist agent was isolated from the endophytic, phyllosphere, rhizosphere, and rhizoplane of Celosia argantea. The results of this study showed that the abundance of bacteria in the phyllosphere of C. argantea was higher than the rhizosphere, rhizoplane, and endophytes, but the diversity was higher in the rhizoplane. Bacterial isolates RP10 and RP 11 have potential as antagonistic agents against Fusarium sp.
Uji Infektivitas Tobacco Mosaic Virus (TMV) dan Cucumber Mosaic Virus (CMV) pada Chaenopodium amaranticolor Siti Shofiya Nasution; Raichan Izzati; Durrah Hayati
Jurnal Media Pertanian Vol 8, No 2 (2023): Oktober
Publisher : Universitas Batanghari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jagro.v8i2.215

Abstract

The infectivity test is one of the completeness in knowing the physical properties of the virus. Determination of the physical properties of the virus can be done by: Thermal inactivation point (TIP), in vitro resistance (Longevity in vitro / LIV), and dilution end point (DEP). The purpose of this study was to determine the infectivity of TMV and CMV in sap through LIV, DEP, and TIP.LIV testing is done by sap incubation until the test time (0 hours, 6 hours, and 24 hours), DEP test is done by diluting sap (not diluted, 10-1, 10-2, 10-3, 10-4, 10-5, 10-6, 10-7), while TIP test is done by heating sap at room temperature, 50oC, 70oC, 90oC for 10 minutes. The results of this study show TMV is stable compared to CMV.TMV is still infectious in sap storage for 24 hours, while CMV is not infectious between 6-24 hours after incubation. The cutoff point of sap TMV dilution is between 10-5 and 10-6, while CMV has been unable to cause symptoms in indicator plants at dilutions between 10-1 and 10-2. TMV is still infectious at 90oC temperature treatment, while CMV inactivation hot spots are 40oC and 50oC.
The Effect of Sheep Dung on the Growth of Odot Grass (Pennisetum purpureum cv. Mott) Pratama, Said Mirza; Nasution, Siti Shofiya; Izzati, Raichan; Zikria, Virda
Jurnal Sain Peternakan Indonesia Vol 18 No 4 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.18.4.209-214

Abstract

Forage plays a vital role because it contains substances needed by livestock that can be used for energy metabolism and to support reproduction. Odot grass is very potent and is one of the varieties of elephant grass that grows not too high so that it can be used as shepherd grass. This study aims to see the effect of sheep dung fertilizer application on plant height, number of leaves and leaf area of Odot grass. This study was an experimental trial designed based on a completely randomized design (RAL), with five treatments and four replications: P0 (without sheep dung), P1 (125 gr/hole), P2 (250 gr/hole), P3 (375 gr/hole), and P4 (500 gr/hole). The results showed that the number of sheep dung did not have a significant effect on all research parameters (height, leaves, and leaf area on Odot grass plants); this was most likely caused by the nutrient conditions of the soil where the research was still relatively extensive and met the needs for the growth of Odot grass, so it was not seen the effect of sheep dung on the growth phase of Odot grass (age 0-60 days).
Co-Authors Alfi Saryanah Durrah Hayati Pratama, Said Mirza Raichan Izzati