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All Journal Majalah Ilmiah METHODA Malahayati International Journal of Nursing and Health Science
Budi Darmanta Sembiring
Universitas Methodist Indonesia
Agriculture, Biological Sciences & Forestry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health

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C-REAKTIVE PROTEIN Budi Darmanta Sembiring
Majalah Ilmiah METHODA Vol. 11 No. 1 (2021): Majalah Ilmiah METHODA
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.656 KB) | DOI: 10.46880/methoda.Vol11No1.pp35-39

Abstract

CRP which is produced in liver is an acute glycoprotein fase that concentrate will increase at the cell injury infection or inflammation. CRP can be use as predictor to primary risk or the second prevention to the heart disease and as a medical evaluation and has prognostic value. The time of CRP checking becomes important to know how long has been happened the disease risk. The CRP measurement can be done by latex-by-latex Agglutination Assay method, Immunoassay dan High Sensitivity C-Reaktif Protein (hs-CRP).
BRAIN NATRIURETIC PEPTIDE (BNP) SEBAGAI BIOMARKER GAGAL JANTUNG KONGESTIF Budi Darmanta Sembiring; Jekson Martiar Siahaan
Majalah Ilmiah METHODA Vol. 10 No. 3 (2020): Majalah Ilmiah METHODA
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.223 KB) | DOI: 10.46880/methoda.Vol10No3.pp130-138

Abstract

Brain Natriuretic Peptide (BNP) is mainly secreted by the heart ventricles and acts as an antagonist to Renin-Angiotensin-Aldosterone. BNP is secreted as pre-proBNP which is broken down into BNP and proBNP in circulation. The BNP examination is done by measuring the levels of BNP or N-Terminal-proBNP depending on the method and the manufacturer. Increased levels of BNP and NT-proBNP indicate heart failure so that BNP and NT-proBNP are considered markers of heart failure. This examination is also indicated to help establish the diagnosis, monitoring, and prognosis of heart failure. Natriuretic peptide examination can be done by several methods such as radioimmunoassay (RIA), Enzyme immunoassay (EIA), Fluorescence immunoassay (FIA), and Sandwich Electro Chemiluminescence Immuno Assay (Sandwich ECLIA).
IMUNOPATOGENESIS DAN MARKER VIRUS HEPATITIS B Budi Darmanta Sembiring; Hendrika Andriana Silitonga
Majalah Ilmiah METHODA Vol. 8 No. 2 (2018): Majalah Ilmiah METHODA
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methoda.Vol8No2.pp31-35

Abstract

Hepatitis B is a sistemic disease which causes infection and necrosis so that it is important to understand about immunophatology and the check meaning of hepatitis B serology markers, expectl liver function test. By doing the serology test can be known the etiology of liver disease besides can be also used to determine the diseases status, also controlling the course of disease and the treatment and also useful from the epidemiology terms. The technique done by examiner of marker Hepatitis B based WHO by method of Radio immuno Assay (RIA), Enzyme linked immuno sorbent Assay (ELISA), Antibody monoclonal
Effectiveness of gram staining and 10% KOH in detecting STIs from anal swabs of sexual assault victims Renatha N.H. Nainggolan; Budi Darmanta Sembiring
Malahayati International Journal of Nursing and Health Science Vol. 9 No. 2 (2026): Volume 9 Number 2
Publisher : Program Studi Ilmu Keperawatan-fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/minh.v9i2.2771

Abstract

Background: Sexually Transmitted Infections (STIs) significantly impact global reproductive health. Sodomy, defined as anal intercourse often involving coercion, poses a high risk for STI transmission. Purpose: To analyze the effectiveness of Gram staining and 10% KOH examinations in detecting pathogens from anal swabs of sexual assault victims. Method: This analytical observational study used a retrospective cross-sectional design. Medical record data from 33 sodomy victims at Dr. Pirngadi Regional Hospital, Medan (2022–2024), were analyzed using total sampling. The reporting of this section adheres to the STROBE. Results: Most victims were male (57.58%), with the 11–20 year age group being most prevalent (69.70%). Gram staining identified Gram-negative diplococci in 17 individuals (51.52%), while 10% KOH detected yeast in 4 individuals (12.12%). McNemar’s analysis yielded a p-value 0.0016 indicates that Gram staining is more effective for STI detection in this context than 10% KOH. Conclusion: Gram staining is a more reliable diagnostic method for detecting STIs in anal swab samples compared to 10% KOH. The high prevalence of bacterial infections highlights the urgent need for early STI screening in sexual assault cases.
Co-Authors Hendrika Andriana Silitonga Renatha N.H. Nainggolan Siahaan, Jekson Martiar