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Fauziatul Fitriyah
Indonesian Oil Palm Research Institute
Agriculture, Biological Sciences & Forestry

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Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets Hayati Minarsih; Fauziatul Fitriyah; Annisa Aulya Aksa; Turhadi Turhadi; Deden Sukmadjaya; Sustiprajitno Sustiprajitno
Menara Perkebunan Vol. 91 No. 1 (2023): 91 (1), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i1.512

Abstract

Dehydrin is known to have an important role in plant response and adaptation to abiotic stresses including drought and high salinity. Previous research reported the isolation of the full-length coding sequence (CDS) of DHN1 from sugarcane var. PSJT 941, and it shares a high homology with DHN genes from sorghum and other sugarcane varieties. In this study, the full-length CDS was cloned under the constitutive CaMV35S promoter and transformed into sugarcane calli mediated by Agrobacterium tumefaciens. The DHN promoter, Pr-1DHNSo, was also successfully isolated from the sugarcane var. PSJT 941 and cloned into the pBI121 expression vector. The promoter construct was subsequently transformed into sugarcane calli of var. Kidang Kencana. Transgenic sugarcane carrying DHN1 gene and DHN promoter constructs were regenerated according to the standard protocol of sugarcane tissue culture. Optimization of an acclimatization protocol using modified post-rooting media was also conducted and the resulting protocol reduced the total mortality rates of the transformed plantlets. The presence of the gene and promoter constructs was periodically tested by PCR using specific primers. The genotyping results showed that the constructs were present for more than a year after transformation.
Synthesis of bio-hydrocarbons pentadecane from crude palm oil (CPO) using recombinant E.coli produced fatty acid photodecarboxylase From Chlorella variabilis Irma kresnawaty; Farhan Palgunadi; Yora Faramitha; Kenny Lischer; Ayu Rahayu Saraswanti; Fauziatul Fitriyah; Djoko Santoso
Menara Perkebunan Vol. 92 No. 2 (2024): 92(2), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i2.592

Abstract

Exploration of natural resources, particularly fossil fuels, is necessary given the sharp rise in energy demand across a wide range of industries. The risk of unpredictable fuel costs, rising pollution, and climate change is elevated as a result of that specific event. In order to address the problem of danger originating from present conventional fuel, it is crucial to use renewable energy that is regarded as sustainable and safe. In the future, bio-hydrocarbons are one energy source that is expected to be used as fuel. In both biological and non-biological processes, biohydrocarbons—hydrocarbons originating from biomass—can be created. Employing the Chlorella variabilis Fatty Acid Photodecarboxylase (CvFAP) enzyme from E. coli recombinant is a remarkable recent technique for producing bio-hydrocarbons. This enzyme has the ability to change free fatty acids, according to extensive studies when induced by blue light and accompanied by the addition of substrates. This study has confirmed the success of producing bio-hydrocarbons in the form of pentadecane with a selectivity of 16.44%. This experiment also indicated that several substantial components are needed in the bio-hydrocarbon synthesis process to obtain an optimal result. The components are the use of TB growth media, the selection of a protein concentration of 1777.5 l ppm, activation time for 3 hours, and the preference for substrate type in the form of 50% CPO.
Co-Authors Annisa Aulya Aksa Ayu Rahayu Saraswanti Deden Sukmadjaya Djoko Santoso Farhan Palgunadi Hayati Minarsih Irma Kresnawaty Kenny Lischer Sustiprajitno Sustiprajitno Turhadi Turhadi Yora Faramitha