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All Journal Jurnal Penelitian Kelapa Sawit Menara Perkebunan
Irma Kresnawaty
Indonesian Oil Palm Research Institute
Agriculture, Biological Sciences & Forestry

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Validasi Marker Infeksi Dini Ganoderma pada Kelapa Sawit di Tiga Lokasi Endemik Ganoderma Galuh Wening Permatasari; Mayumi Puspita; Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Happy Widiastuti; Kuwat Triyana; Priyono Priyono
Jurnal Penelitian Kelapa Sawit Vol 31 No 1 (2023): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v31i1.195

Abstract

A serious disease that affects the viability of the oil palm industry is basal stem rot, which is caused by Ganoderma. The current level of disease can be viewed as unmanageable, given that the palms were growing in an unfavorable or unsuitable climate. Today, there are numerous approaches to diagnose diseases early, and one of them using molecular methods. Seven genes for early infection markers were effectively generated by a reference's transcriptome study, including LEUCO, ETHYLENE, CHALCONE, ANTHOCYANIDIN, ETHYLENE, MANNOSE, and SENESCENCE. The purpose of this study is to validate and confirm the presence of Ganoderma infections in three endemic oil palm field in Indonesia i.e. Cisalak Baru, Rejosari, and Bekri plantation. This study conducted real time qPCR of RNA from oil palm roots with four different severities of infection. Manual processing of RNA isolation and cDNA synthesis were carried out, to provide quantification expression level. In addition, gene ontology (GO) analysis was also performed in order to explain the roles of each gene tested. The results revealed that CHALCONE is the only marker that consistently elucidate the Ganoderma's early infection appear in three locations. The drawbacks of the analysis results are tightly correlating to the age of oil palm as well as endemic location. GO results declare that seven genes function related to the response of infection. This work was successful in confirming early infection in three places, elucidating the variables influencing the efficacy and sensitivity of molecular detection, and revealing the function and importance of particular genes for detection.
Synthesis of bio-hydrocarbons pentadecane from crude palm oil (CPO) using recombinant E.coli produced fatty acid photodecarboxylase From Chlorella variabilis Irma kresnawaty; Farhan Palgunadi; Yora Faramitha; Kenny Lischer; Ayu Rahayu Saraswanti; Fauziatul Fitriyah; Djoko Santoso
Menara Perkebunan Vol. 92 No. 2 (2024): 92(2), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i2.592

Abstract

Exploration of natural resources, particularly fossil fuels, is necessary given the sharp rise in energy demand across a wide range of industries. The risk of unpredictable fuel costs, rising pollution, and climate change is elevated as a result of that specific event. In order to address the problem of danger originating from present conventional fuel, it is crucial to use renewable energy that is regarded as sustainable and safe. In the future, bio-hydrocarbons are one energy source that is expected to be used as fuel. In both biological and non-biological processes, biohydrocarbons—hydrocarbons originating from biomass—can be created. Employing the Chlorella variabilis Fatty Acid Photodecarboxylase (CvFAP) enzyme from E. coli recombinant is a remarkable recent technique for producing bio-hydrocarbons. This enzyme has the ability to change free fatty acids, according to extensive studies when induced by blue light and accompanied by the addition of substrates. This study has confirmed the success of producing bio-hydrocarbons in the form of pentadecane with a selectivity of 16.44%. This experiment also indicated that several substantial components are needed in the bio-hydrocarbon synthesis process to obtain an optimal result. The components are the use of TB growth media, the selection of a protein concentration of 1777.5 l ppm, activation time for 3 hours, and the preference for substrate type in the form of 50% CPO.
Co-Authors Agustin Sri Mulyatni Ayu Rahayu Saraswanti Deden Dewantara Eris Djoko Santoso Farhan Palgunadi Fauziatul Fitriyah Galuh Wening Permatasari Happy Widiastuti Kenny Lischer Kuwat Triyana Mayumi Puspita Priyono Priyono Yora Faramitha