Article Per Year (5 Year)
p-Index From 2021 - 2026
0.888
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Kelapa Sawit Menara Perkebunan
Irma Kresnawaty
Indonesian Oil Palm Research Institute
Agriculture, Biological Sciences & Forestry

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Validasi Marker Infeksi Dini Ganoderma pada Kelapa Sawit di Tiga Lokasi Endemik Ganoderma Galuh Wening Permatasari; Mayumi Puspita; Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Happy Widiastuti; Kuwat Triyana; Priyono Priyono
Jurnal Penelitian Kelapa Sawit Vol 31 No 1 (2023): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v31i1.195

Abstract

A serious disease that affects the viability of the oil palm industry is basal stem rot, which is caused by Ganoderma. The current level of disease can be viewed as unmanageable, given that the palms were growing in an unfavorable or unsuitable climate. Today, there are numerous approaches to diagnose diseases early, and one of them using molecular methods. Seven genes for early infection markers were effectively generated by a reference's transcriptome study, including LEUCO, ETHYLENE, CHALCONE, ANTHOCYANIDIN, ETHYLENE, MANNOSE, and SENESCENCE. The purpose of this study is to validate and confirm the presence of Ganoderma infections in three endemic oil palm field in Indonesia i.e. Cisalak Baru, Rejosari, and Bekri plantation. This study conducted real time qPCR of RNA from oil palm roots with four different severities of infection. Manual processing of RNA isolation and cDNA synthesis were carried out, to provide quantification expression level. In addition, gene ontology (GO) analysis was also performed in order to explain the roles of each gene tested. The results revealed that CHALCONE is the only marker that consistently elucidate the Ganoderma's early infection appear in three locations. The drawbacks of the analysis results are tightly correlating to the age of oil palm as well as endemic location. GO results declare that seven genes function related to the response of infection. This work was successful in confirming early infection in three places, elucidating the variables influencing the efficacy and sensitivity of molecular detection, and revealing the function and importance of particular genes for detection.
Synthesis of bio-hydrocarbons pentadecane from crude palm oil (CPO) using recombinant E.coli produced fatty acid photodecarboxylase From Chlorella variabilis Irma kresnawaty; Farhan Palgunadi; Yora Faramitha; Kenny Lischer; Ayu Rahayu Saraswanti; Fauziatul Fitriyah; Djoko Santoso
Menara Perkebunan Vol. 92 No. 2 (2024): 92(2), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i2.592

Abstract

Exploration of natural resources, particularly fossil fuels, is necessary given the sharp rise in energy demand across a wide range of industries. The risk of unpredictable fuel costs, rising pollution, and climate change is elevated as a result of that specific event. In order to address the problem of danger originating from present conventional fuel, it is crucial to use renewable energy that is regarded as sustainable and safe. In the future, bio-hydrocarbons are one energy source that is expected to be used as fuel. In both biological and non-biological processes, biohydrocarbons—hydrocarbons originating from biomass—can be created. Employing the Chlorella variabilis Fatty Acid Photodecarboxylase (CvFAP) enzyme from E. coli recombinant is a remarkable recent technique for producing bio-hydrocarbons. This enzyme has the ability to change free fatty acids, according to extensive studies when induced by blue light and accompanied by the addition of substrates. This study has confirmed the success of producing bio-hydrocarbons in the form of pentadecane with a selectivity of 16.44%. This experiment also indicated that several substantial components are needed in the bio-hydrocarbon synthesis process to obtain an optimal result. The components are the use of TB growth media, the selection of a protein concentration of 1777.5 l ppm, activation time for 3 hours, and the preference for substrate type in the form of 50% CPO.
Characterization of sn-1,3 extracellular lipases of Aspergillus niger and Rhizopus oryzae for the crude palm oil hydrolysis Ludwinardo Putra; Eneng Nurhasanah; Eti Rohaeti; Irma Kresnawaty
E-Journal Menara Perkebunan Vol. 93 No. 2 (2025): 93(2), 2025
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i2.673

Abstract

Crude palm oil (CPO) processing for sn-2 palmitate synthesis requires a specific sn-1,3 lipase to catalyze the hydrolysis step. These lipases are known to be derived from filamentous fungal isolates including Aspergillus niger and Rhizopus oryzae. The purpose of this study was to describe extracellular lipases from A. niger and R. oryzae that hydrolyze CPO. Extracellular lipases were successfully recovered from both fungal isolates using CPO-enriched fermentation media. The crude fraction was partially refined using (NH4)2SO4 and dialyzed, yielding two fractions. Lipase fraction I from both fungal species had the highest specific activity, had a molecular mass of ~30 kDa, and was sn-1,3 specific. The best conditions for enzyme activity of both fungal species in CPO hydrolysis were pH 6-7 and 35 ℃. The pH and heat stability of lipase fraction I in both fungi were relatively low. However, this enzyme worked effectively in benzene, ethanol, and methanol solvents.
Biochemical and gene expression analysis for the selection of elite oil palm ortets Irma Kresnawaty; Ludwinardo Putra; Heni Rahmawati; Zulhan Arif; Masna Maya Sinta; Rizka Tamania Saptari; Fauziatul Fitriyah; Heri Adriwan Siregar; Imron Riyadi
E-Journal Menara Perkebunan Vol. 94 No. 1 (2026): 94(1), 2026
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v94i1.676

Abstract

The rising global demand for palm oil necessitates innovative strategies to accelerate yield improvement and ensure sustainable production. Conventional propagation methods are limited by long regeneration cycles, making tissue culture a more practical alternative that relies on elite ortet identification. This preliminary study aimed to evaluate crude palm oil quality, leaf metabolite profiles, and gene expression as early indicators for selecting elite ortets. Mature fruits were used to assess oil yield, free fatty acid, and β-carotene contents, while leaves were analyzed for chlorophyll and total phenolic content. Based on biochemical screening, three candidate ortets were further evaluated for the expression of acetyl-CoA carboxylase (ACCase) and phytoene synthase (PSY) genes. Oil yield ranged from 77-83%, FFA 5-9%, and β-carotene 200-450 mg kg-1 CPO across candidates. Candidate 3.7 exhibited the highest oil yield and elevated ACCase expression, while candidate 6.12 showed the highest β-carotene content and PSY expression. Candidate 5.7 demonstrated relatively low FFA and favorable chlorophyll levels. These results suggest an association between ACCase expression and oil yield, as well as between PSY expression and β-carotene accumulation. Collectively, these findings support a proof-of-concept for an integrated profiling strategy to select elite ortets for advanced tissue culture propagation.
Co-Authors Agustin Sri Mulyatni Ayu Rahayu Saraswanti Deden Dewantara Eris Djoko Santoso Eneng Nurhasanah Eti Rohaeti Farhan Palgunadi Fauziatul Fitriyah Galuh Wening Permatasari Happy Widiastuti Heni Rahmawati Heri Adriwan Siregar Imron Riyadi Kenny Lischer Kuwat Triyana Ludwinardo Putra Masna Maya Sinta Mayumi Puspita Priyono Priyono Rizka Tamania Saptari Yora Faramitha Zulhan Arif