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All Journal JURNAL KIMIA SAINS DAN APLIKASI
Sumariyah Sumariyah
Department of Physics, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

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Phytochemical Test, Determination of Total Phenol, Total Flavonoids and Antioxidant Activity of Ethanol Extract of Moringa Leaves (Moringa oleifera Lam) Enny Fachriyah; Dewi Kusrini; Ifan Bagus Haryanto; Synta Mutiara Bunga Wulandari; Widyaningrum Islami Lestari; Sumariyah Sumariyah
Jurnal Kimia Sains dan Aplikasi Vol 23, No 8 (2020): Volume 23 Issue 8 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (22.509 KB) | DOI: 10.14710/jksa.23.8.290-294

Abstract

Moringa (Moringa oleifera Lam) is a medicinal plant that is a source of nutrients. It is rich in protein, fatty acids, minerals, relatively high polyphenol compounds, and has antioxidant activity. This study aims to obtain information about secondary metabolites, total phenol content, total flavonoids, and antioxidant activity of the ethanol extract of Moringa leaves. Total phenol was determined by the Folin–Ciocâlteu method, while total flavonoids were determined by the AlCl3 colorimetric method. Whilst the antioxidant activity was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method at a wavelength of 517 nm. The results showed that the ethanol extract of Moringa leaves contained flavonoids, phenols, tannins, saponins, alkaloids, and steroids. The total phenol content in the ethanol leaf extract was 63.16 mg GAE/g extract, and the total flavonoid content was 10.477 mg QE/g extract. Moringa leaf ethanol extract has antioxidant activity with an IC50 of 118.6145 mg/L, classified as having moderate antioxidant activity.
Co-Authors Dewi Kusrini Enny Fachriyah Ifan Bagus Haryanto Synta Mutiara Bunga Wulandari Widyaningrum Islami Lestari