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All Journal International Journal of Basic and Applied Science Jurnal Informasi Sains dan Teknologi (ISAINTEK) Indonesian Journal of Life Sciences
Mario Donald Bani
Department Of Biotechnology, Indonesia International Institute For Life-Sciences, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Medicine & Pharmacology Physics Other

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The Effects Of Planting Distance Treatment And Zeolite Application As Ameliorants On Growth And Yield Of Bok Choy Plants Mario Donald Bani; Gregorio Antonny Bani
Jurnal Informasi, Sains dan Teknologi Vol. 6 No. 1 (2023): Juni: Jurnal Informasi Sains dan Teknologi
Publisher : Politeknik Negeri FakFak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/isaintek.v6i1.76

Abstract

The increasing market demand for Bok choy makes the need to apply a good cultivation system for the optimum growth and productivity of the plants very important. One of the main factors that must be considered in cultivating Bok choy is the treatment of plant spacing in which the soil is often enriched with ameliorants, such as zeolite. The combination treatment of plant spacing and zeolite showed a significant effect on the growth and productivity of Bok choy plants, where the further the plant spacing, the higher the growth rate. However, the same plant spacing treatment did not affect the pH value. The best zeolite treatment was 150 g/1x5 m2 soil bed, or 300 kg/ha.
Ende natural zeolite as a catalyst in the biodiesel production from nyamplung oil Gregorio Antonny Bani; Mario Donald Bani
International Journal of Basic and Applied Science Vol. 12 No. 2 (2023): Sep: Basic and Applied Science
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/ijobas.v12i2.250

Abstract

Fuel use as a primary energy source is increasing daily, and the reserves of these natural resources are diminishing quickly. Different studies have been performed to see the potential use of the seeds of the Nyamplung plant (Chalophyllum inophyllum L.) as biodiesel. To produce biodiesel from Nyamplung oil, a catalyst, such as zeolite, is often required to speed up the reaction, save energy use, and increase the quantity and quality of the biodiesel. There is a high variation in terms of natural zeolite’s catalytic activity, depending on the location where the zeolite was formed in the past. The objectives of this study were to characterize and understand the Ende natural zeolite, before and after activation, and to see the catalytic activity of the HCl-activated zeolite in the production of biodiesel from Nyamplung oil. When zeolite was used as a catalyst, optimum effects on the esterification were observed at 700 rpm stirring speed with a reaction time of 15 minutes. These conditions optimally converted 100% of Nyemplung oil into biodiesel.
The Potential Use of Algae as Biosorbents for Mercury Removal in the Indonesian Water Bodies Josefano, Richelle Bertly; Belva, Felicia Lael; Yoel, Abigail; Rahardja, Richelle Tirta; Dharmawan, Nethania Angeline; Tjandra, Noah William; Bani, Mario Donald
Indonesian Journal of Life Sciences 2023: IJLS Vol 05 No .02
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v5i02.164

Abstract

Contamination of mercury in the water body in Indonesia has become a big concern for many people due to the harmful effects of this heavy metal when it enters the body. Mercury contamination may cause neurological disorders that lead to loss of senses, and damage the brain, the central nervous system, the kidney, and can lead to birth defects. Human activities, such as Coal-Fired Power Plants (CFFPs) and Artisanal and Small-Scale Gold Mining (ASGM) as well as other mining activities, are among the biggest contributors of mercury emissions in Indonesia’s water body. Biosorbents such as fungi, bacteria and algae can be utilized to alleviate this problem, with algae being the most reliable biosorbent due to its abundance in Indonesia, low cost manufacturing, and high metal ion binding capacity. There are three varieties of algae that can be used as a mercury biosorbent: green algae (Chlorophyta), red algae (Rhodophyta), and brown algae (Phaeophyta). Different studies have shown that the most compatible mercury biosorbent is green algae due to its highest mercury absorption capacity. However, there are limited studies about the use of algae in Indonesia as mercury biosorbent.
In Silico Evaluation of the Inhibition of Pseudomonas aeruginosa Biofilm Production in Burn Wound Infections Using CATH-2 and LL-37 Peptides Adhiwijaya, Priscilla Klaresza; Kaitlyn, Gabriella Zevania; Valenthenardo, Louis; Anjani, Patricia Tiara; Bani, Mario Donald
Indonesian Journal of Life Sciences 2025: IJLS Vol 07 No.02
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v7i02.238

Abstract

Patients with burn injuries are at high risk of bacterial infection due to the loss of the skin barrier, often leading to complications that contribute to increasing death tolls from burn injuries. The formation of biofilms in bacteria increases its survival rate, especially in the rise of antibiotic resistance cases, which ineffectively combats biofilm production. This research explores the use of two types of cationic antimicrobial peptides, LL-37 and CATH2, commonly originating from humans and chickens, respectively, as a form of host defense in preventing the formation of biofilms by one of the most common pathogenic bacterial strains in severe burn wounds, Pseudomonas aeruginosa, through inhibition in its LPS region. In silico analyses were performed using AlphaFold, GLYCAM-Web, YASARA, and AutoDock Vina. It was found that the CATH-2 model has the strongest binding affinity towards the three types of LPS—alginate, Pel, and Psl—scoring between -5.5 and -6.0 kcal/mol, as opposed to the score range of -4.1 to -6.0 for LL-37. However, the LL-37 model is considered more precise than the CATH-2 model overall, meaning the in silico results of the former are likely more accurate in real life than the latter. These results suggest the utilization of the two peptides as treatments in severe burn cases. In future developments, the application of genetically engineered plasmid-inserted Staphylococcus epidermidis, a commensal bacterium commonly found on human skin, to produce peptides may be considered.
Preliminary in silico study of a novel paratransgenic weapon against malaria: Genetically modifying wild plasmodium populations via recombinant mosquito symbiont Sun, Joshua; Candra, Daniel Christopher; Pramanda, Ihsan Tria; Bani, Mario Donald; Wu, Nicholas; Husada, William; Alexander, Jonathan; Saputra, Silvan; Aberly, Rachel Sofian; Fidelia, Michelle
International Journal of Basic and Applied Science Vol. 12 No. 4 (2024): March: Basic and Applied Science
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/ijobas.v12i4.305

Abstract

Malaria is an infectious disease caused by Plasmodium spp., a protist whose infection is spread by Anopheles mosquito as a vector. A potential method to counter the infection is through paratransgenesis, a promising genetic control approach. This study proposed a new approach by using a transgene carried in a modified Ti plasmid hosted in Asaia bacterium to carry out two purposes: sensing the presence of Plasmodium protein biomarkers and transferring the toxin transgene to the parasite upon detection, thereby killing it without harming the Asaia carrier. The biosensor mechanism was created by repurposing a TonB dependent iron-uptake transport pathway to transcribe the vir genes of Agrobacterium tumefaciens that facilitate the gene transfer. The aim of this in silico project was to serve as a preliminary study on the likelihood of success of the aforementioned biosensor mechanism. This study utilized Alphafold and RCSB (Research Collaboratory for Structural Bioinformatic Protein Data Bank). As for the research tools: PyMOL, ClusPro website, and PRODIGY (PROtein binDIng enerGY prediction) server was used for data preparation, protein-protein docking and binding affinity analysis respectively. The results were assessed with t-test to analyze the significance of the binding affinity, in comparison to other studies that employed similar methods. The result showed Plasmodium protein GGCS (Gamma-glutamylcysteine synthetase) having the highest binding affinity with FecA (Fe(3+) dicitrate transport protein A). The preliminary data suggested that introducing the toxin transgene may be possible through vir gene transcription from the TonB pathway.
Co-Authors Aberly, Rachel Sofian Adhiwijaya, Priscilla Klaresza Alexander, Jonathan Anjani, Patricia Tiara Belva, Felicia Lael Candra, Daniel Christopher Dharmawan, Nethania Angeline Fidelia, Michelle Gregorio Antonny Bani Husada, William Josefano, Richelle Bertly Kaitlyn, Gabriella Zevania Pramanda, Ihsan Tria Rahardja, Richelle Tirta Saputra, Silvan Sun, Joshua Tjandra, Noah William Valenthenardo, Louis Wu, Nicholas Yoel, Abigail