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All Journal International Journal of Basic and Applied Science Indonesian Journal of Life Sciences
Pramanda, Ihsan Tria
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Medicine & Pharmacology Physics

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Inulin-producing Genes in Gembili (Dioscorea esculenta) and Future Applications for Food Industries in Indonesia Pramanda, Ihsan Tria; Anjani, Dellamartha; Heriawan, Gili
Indonesian Journal of Life Sciences 2022: IJLS Vol 04 No .01
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (965.162 KB) | DOI: 10.54250/ijls.v4i1.133

Abstract

Inulin can be found in abundance in nature, this form of carbohydrate (fructans) is utilized by plants as an energy storage and possibly for other uses. Human use inulin as a dietary fiber and prebiotics to improve the health of their digestive system or to improve the physicochemical and sensory properties of foods. Gembili (Dioscorea esculenta) is a type of yam that grows well in Indonesia and contains inulin up to 14.77% of its dry weight. The current state of inulin production utilizes conventional extraction from tubers; thus, the demands of the market cannot be satisfied, and the price skyrocketed. Biotechnological approaches such as incorporating genes that encodes the enzymes involved in inulin biosynthesis into bacteria or yeasts can be exploited to improve the yield and sustainability. The biosynthesis of inulin involves three main genes 1-SST, 6-SFT and 1-FFT. Genetic information of the gene responsible for the biosynthesis of inulin in Gembili needs to be elucidated.
Process Modeling and Techno-Economic Analysis of Xylitol Production from Oil Palm Empty Fruit Bunch (OPEFB) using SuperPro Designer® Salam, Wildan Qoharisma; Pramanda, Ihsan Tria; Harjanto, Fariz Hutama Putra; Rukmana, Jaka
Indonesian Journal of Life Sciences 2023: IJLS Vol 05 No .02
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v5i02.183

Abstract

The utilization of OPEFB as a biomass waste from the crude palm oil industry offers a potential avenue for producing xylitol through biotechnological processes. The production of xylitol involves a series of steps, including hydrolysis, fermentation, and purification. Xylitol, a sugar alcohol used as a food sweetener, is obtained in crystalline form. However, the fermentation process generates impurities that need to be separated to achieve a high purity level of xylitol. To address this, a combined approach of membrane distillation and cooling batch crystallization was employed in this study. The feasibility of establishing a xylitol production plant was assessed through simulation and techno-economic analysis using SuperPro Designer software version 12. The plant was designed to produce 3.00 MT/batch of xylitol as the primary product, requiring 20 MT/batch of OPEFB. Each batch operation lasted for seven days, with a batch cycle occurring every three days. The simulation was evaluated based on the annual operating cost (AOC). The project incurred a total investment of $2.00 MM, with an annual operating cost of $3.78 MM, and generated yearly revenues of $6.84 MM. The economic analysis revealed that the plant was economically viable, with a payback period of 11 months, a gross margin of 44.71%, and an internal rate of return (IRR) of 62.26%.
Preliminary in silico study of a novel paratransgenic weapon against malaria: Genetically modifying wild plasmodium populations via recombinant mosquito symbiont Sun, Joshua; Candra, Daniel Christopher; Pramanda, Ihsan Tria; Bani, Mario Donald; Wu, Nicholas; Husada, William; Alexander, Jonathan; Saputra, Silvan; Aberly, Rachel Sofian; Fidelia, Michelle
International Journal of Basic and Applied Science Vol. 12 No. 4 (2024): March: Basic and Applied Science
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/ijobas.v12i4.305

Abstract

Malaria is an infectious disease caused by Plasmodium spp., a protist whose infection is spread by Anopheles mosquito as a vector. A potential method to counter the infection is through paratransgenesis, a promising genetic control approach. This study proposed a new approach by using a transgene carried in a modified Ti plasmid hosted in Asaia bacterium to carry out two purposes: sensing the presence of Plasmodium protein biomarkers and transferring the toxin transgene to the parasite upon detection, thereby killing it without harming the Asaia carrier. The biosensor mechanism was created by repurposing a TonB dependent iron-uptake transport pathway to transcribe the vir genes of Agrobacterium tumefaciens that facilitate the gene transfer. The aim of this in silico project was to serve as a preliminary study on the likelihood of success of the aforementioned biosensor mechanism. This study utilized Alphafold and RCSB (Research Collaboratory for Structural Bioinformatic Protein Data Bank). As for the research tools: PyMOL, ClusPro website, and PRODIGY (PROtein binDIng enerGY prediction) server was used for data preparation, protein-protein docking and binding affinity analysis respectively. The results were assessed with t-test to analyze the significance of the binding affinity, in comparison to other studies that employed similar methods. The result showed Plasmodium protein GGCS (Gamma-glutamylcysteine synthetase) having the highest binding affinity with FecA (Fe(3+) dicitrate transport protein A). The preliminary data suggested that introducing the toxin transgene may be possible through vir gene transcription from the TonB pathway.
Co-Authors Aberly, Rachel Sofian Alexander, Jonathan Anjani, Dellamartha Candra, Daniel Christopher Fidelia, Michelle Harjanto, Fariz Hutama Putra Heriawan, Gili Husada, William Jaka Rukmana Mario Donald Bani Saputra, Silvan Sun, Joshua Wildan Qoharisma Salam Wu, Nicholas