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All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology Jurnal Ilmu Kehutanan Jurnal Manusia dan Lingkungan
Sena Adi Subrata
Faculty of Forestry, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Social Sciences

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Tingkat Kesukaan Rusa Bawean (Axis kuhlii) terhadap Jenis-Jenis Tumbuhan Bawah di Suaka Margasatwa dan Cagar Alam Pulau Bawean Sena Adi Subrata; Muhammad Faddel Jauhar
Jurnal Ilmu Kehutanan Vol 1, No 2 (2007)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.001 KB) | DOI: 10.22146/jik.1554

Abstract

Level of Food Preference of Bawean Deer (Axis kuhlii) on Food Species in Wildlife Sanctuary and Nature Reserve of Bawean IslandThis research has a background on the importance of food preference of Rusa Bawean (Axis kuhlii). This information can be used to ensure the adequacy of preferred food availability within their habitat. This paper aimed to estimate food preference of Rusa Bawean employing faecal analysis. The technique was selected because it is safe for the animal and is easy in data analysis and interpretation. To achieve the aim, quasi experimental design was applied to 30 vegetation plots and 60 faecal samples. Data was collected during January 2005 and June 2006 in the area of Wildlife Sanctuary and Nature Reserve of Bawean Island. Data was analyzed with chi-square test and food selection index. Result shows Rusa Bawean have preferences on some of food species, and they do not select their food species randomly according to its abundance.  Preferred food species include Kabak-kabakan alas (Brachiaria distachya), Talioar (Panicum cordayum), Gadung (Dioscorea hispida), Andudur (Caryota mitis), Rombok putih (Merremia peltata), Pele (Urena lobata) and Kabak-kabakan merah (Ischaemum timorense). Whereas avoided food species include Lating-latingan (Scleria hebecarpa), Ancucu (Pericampylus glaucus), Lambu Merah (Paspalum conjugatum), Alang-alang (Imperata cylindrica), Padi-padian (Centotheca lappacea), Lente-lentean (Fimbristylis dichotoma), Taliata (Lygodium circinnatum ) dan Kayu sape (Symplocos adenophylla).
Seleksi Pohon untuk Sarang Kuntul Kerbau (Bubulcus ibis) di Dusun Wisata Ketingan Kab. Sleman Daerah Istimewa Yogyakarta Eko Kosasih; Sena Adi Subrata
Jurnal Ilmu Kehutanan Vol 5, No 2 (2011)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (744.396 KB) | DOI: 10.22146/jik.1851

Abstract

Penelitian seleksi pohon untuk sarang Kuntul kerbau (Bubulcus ibis) di desa Ketingan, Kabupaten Sleman, Yogyakarta bertujuan untuk menyediakan informasi dasar dalam manajemen populasi. Survei keberadaan sarang Kuntul kerbau dilakukan bersama dengan identifikasi ketersediaan jenis pohon. Pengukuran tingi pohon, ukuran tajuk, kerapatan tajuk dan jarak dari gangguan manusia juga dilakukan untuk masing-masing pohon. Uji Kai-kuadrat menunjukkan bahwa Kuntul kerbau memilih beberapa jenis pohon untuk bersarang seperti Gnetum gnemon L., Cassia siamea Lamk. Artocarpus heterophyllus Lamk., Callophyllum inophyllum L., Melia azedarach L., Antidesma bunius (L.) Spreng., dan Dysoxylum gaudichaudianum (Juss.) Miq. Tinggi pohon, tinggi tajuk, diameter dan jarak dari gangguan manusia menunjukkan perbedaan nyata antara pohon yang digunakan dan tidak digunakan untuk sarang tetapi diameter setinggi dada dan kerapatan tajuk pohohn tidak menunjukkan perbedaan nyata. Manajemen habitat Kuntul kerbau diperlukan dalam pengendalian populasi untuk konservasi jenis burung ini.Kata kunci : penggunaan sumberdaya, ketersediaan sumberdaya, seleksi habitat, Bubulcus ibis Tree Selection for Nest of Cattleengret (Bubulcus ibis) in Ketingan Tourisme Village, Sleman Regency, YogyakartaAbstractHabitat selection of Bubulcus ibis in Ketingan Village, Sleman Regency, Yogyakarta was investigated to provide basic information for population management. Presence of Bubulcus ibis nest was surveyed along with identification available tree species. Tree height, canopy size and openness, and distance from human disturbance were also measured for each tree. The Chi Square test showed that bubulcus ibis selected several species as nest trees such as Gnetum gnemon L., Cassia siamea Lamk., Artocarpus heterophyllus Lamk., Calophyllum inophyllum L., Melia azedarach L., Antidesma bunius (L.) Spreng., and Dysoxylum gaudichaudianum(Juss.) Miq. Tree height, canopy height, canopy diameter and the distance from human disturbance showed a significant difference between used and non-used trees for the nest, however diameter breast high (DBH) and canopy openness of trees did not show significant differences. Habitat management of Bubulcus ibis should be improved by controlling its population that needed for the conservation of this bird species.
PCR Primer Spesifik Berdasarkan Gen Cytochrome b untuk Deteksi Garangan (Herpestes javanicus) secara Molekuler Sena Adi Subrata; Subeno Subeno; Atus Syahbudin
Jurnal Ilmu Kehutanan Vol 14, No 1 (2020)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.356 KB) | DOI: 10.22146/jik.57463

Abstract

Garangan (Herpestes javanicus) merupakan salah satu spesies meso-carnivora yang berperan penting dalam ekosistem sebagai pemangsa. Kehadirannya dianggap mampu mengendalikan populasi spesies mangsa, termasuk beberapa spesies hama. Namun anggapan ini dianggap hanya anekdot karena kekurangan data ekologis pendukungnya sebagai dampak dari kesulitan pengamatan visual atas spesies ini. Penelitian ini bertujuan untuk merancang PCR primer untuk deteksi Garangan secara molekuler dari material organik yang ditinggalkan, misalnya kotoran. Perancangan PCR primer dimulai dengan memilih penanda spesifik Garangan yang melibatkan 51 sekuen DNA gen Cytochrome-b dari 19 spesies karnivora Jawa, menentukan primer forward dan reverse, dan menguji in-silico dan in vitro dari primer yang berhasil dirancang. Proses tersebut dilakukan dengan bantuan software MEGA 5 dan SP-Designer, dan memanfaatkan basis data genetik dari GenBank (NCBI). Uji in silico dan in vitro menunjukkan bahwa sekuen primer forward 5’- CAAATCACACCCACTCATTAAAATC-3’ dan reverse 5’-TGTGGGTTACTGATGAAAAGG-3’ akan mampu mendeteksi Garangan secara molekuler dari material organik spesies ini. Penelitian ini berkontribusi dalam pengumpulan data dasar kehadiran Garangan untuk mengumpulkan informasi lanjut tentang okupansi, distribusi spesies, dan pakannya. Informasi ini merupakan fondasi untuk memahami peran spesies ini dalam ekosistem. Specific PCR Primers Based on Cytochrome-b Gene for Molecular Detection of JavanMongoose (Herpestes javanicus)AbstractJavan Mongoose is a meso-carnivore species that have an important role in ecosystem as a predator. It is believed that its occurrence controls prey populations including some pest species. However, the belief is an anecdote because of lacking supportive data. Difficulty in visually observing the species is a major problem preventing data collection. This study aims to design PCR primer for detecting the Mongoose molecularly from organic material remaining, such as feces. The design starts with selecting specific marker from 51 DNA sequences of cytochrome-b. The DNA sequences were of 19 Javan carnivore collected from GenBank (NCBI). PCR primers were designed and tested using both in-silico and in-vitro techniques. The sequence collection and selection, and primer design process employed MEGA 5 and SP-Designer software. We successfully designed PCR primers: forward 5’- CAAATCACACCCACTCATTAAAATC-3’ dan reverse 5’-TGTGGGTTACTGATGAAAAGG-3’. The primer is capable of detecting Javan Mongoose from remaining organic material. The study contributed to basic data collection of Javan Mongoose for advanced studies such as occupancy modeling, species distribution modeling and diet analysis. This information is fundamental for understanding the role of the Javan Mongoose in an ecosystem.
Diet Composition and Overlap of Two Mesocarnivores, Leopard Cat (Prionailurus bengalensis) and Javan Mongoose (Urva javanica), in Java, Indonesia Sena Adi Subrata; Ulfah Darmayani Permatasari
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.596-605

Abstract

Dietary study of carnivores has garnered a great deal of attention for a very long time since it provides information for comprehending ecological processes and tracking the economic effects of animals on food production. Few studies reported alternative prey that is detrimental to food production, such as rodents and insects, in addition to the numerous studies on livestock predation. Therefore, this study aims to provide information on the diet composition and overlap of two mesocarnivore species, Javan mongoose (Urva javanica) and leopard cat (Prionailurus bengalensis), in Java, Indonesia. The macroscopic observation technique was applied for identifying food remnants found in the 90 feces of the mongoose and 100 feces of the cat collected in 2018. Twenty three and 21 taxa of prey were found in the leopard and Javan mongoose feces. Rodentia was the most frequent taxa found in the feces of both species, followed by Galliformes. Insects comprised the majority of diet items but at low frequencies. Two mesocarnivores were specialists (Levin’s index of leopard cat: 0.08; Javan mongoose: 0.26) with fairly high diet overlap (Pianka’s index = 0.78). The results provided compelling evidence of the positive and negative impacts of mesocarnivores on food production in Java.
PERAN TUMBUHAN BAWAH DALAM KESUBURAN TANAH DI HUTAN PANGKUAN DESA PITU BKPH GETAS (The Role of Undergrowth Species for Soil Fertility in Hutan Pangkuan Desa Pitu BKPH Getas) Frita Kusuma Wardhani; Ikhwanudin Rofi’i; Ambar Kusumandari; Sena Adi Subrata; Kristiani Fajar Wianti
Jurnal Manusia dan Lingkungan Vol 27, No 1 (2020): 1
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.49668

Abstract

AbstrakKehadiran tumbuhan bawah pada hutan tanaman jati dapat memberikan dampak positif yaitu, menjadi sumber keragaman hayati, menciptakan iklim mikro di lantai hutan, menjaga tanah dari bahaya erosi, serta dapat memelihara kesuburan tanah. Namun keberadaannya seringkali dianggap sebagai kompetitor bagi tanaman yang dibudidayakan. Hutan Pangkuan Desa (HPD) Pitu telah dikelola secara intensif oleh masyarakat yang dilakukan baik di bawah maupun di luar tegakan sehingga diduga mempengaruhi kondisi ekosistem di kawasan tersebut baik terhadap kelimpahan tumbuhan bawah maupun kualitas kimia tanahnya. Tujuan penelitian ini adalah untuk mengetahui keanekaragaman jenis tumbuhan bawah dan kualitas kimia tanah pada berbagai tipe penutupan lahan di HPD Pitu, BKPH Getas. Pengamatan dilakukan dengan menempatkan petak kuadrat berukuran 1 m x 1 m sebanyak 5 petak pada setiap unit lahan secara random. Hasil penelitian menunjukkan pada 4 (empat) penutupan lahan yaitu hutan, semak, ladang, dan pemukiman didominasi oleh jenis yang berbeda tetapi komposisi komunitas pada hutan dan semak memiliki similaritas yang relatif tinggi. Kualitas kimia tanah pada berbagai penutupan lahan tidak berbeda secara signifikan. Aktivitas masyarakat yang tinggi pada berbagai penutupan lahan diduga menjadi penyebab rendahnya hara pada berbagai tipe penutupan. Kondisi keharaan yang miskin hara khususnya pada kawasan hutan tentu akan memengaruhi produktivitas tegakan jati yang merupakan tanaman pokok di kawasan tersebut. AbstractThe presence of undergrowth on teak plantations can have a positive impact i.e, a source of biodiversity, creating a microclimate on the forest floor, keeping the soil from the erosion, and can maintain soil fertility. On the other hand, its existence is often regarded as a competitor for cultivated plants. Hutan Pangkuan Desa (HPD) Pitu has been intensively managed by communities undertaken either below or outside the stand so that it is suspected to affect the ecosystem condition in the region both to the abundance of plants and quality of soil chemistry. The purpose of this study was to determine the diversity of undergrowth species and soil chemical qualities in various types of land cover in HPD Pitu, BKPH Getas. The observation was done by placing a 1 m x 1 m square plot of 5 plots on each unit of land. The results showed that 4 (four) land closures i.e forests, shrubs, dry farmland, and settlements were dominated by different species but the community composition in forests and shrubs had a relatively high similarity. The soil chemical quality at various land coverings did not differ significantly. High community activity in various land closures is thought to be the cause of low nutrients in various types of closure. The nutrient-poor condition of the forest, especially in the forest area, will affect the productivity of teak stands, which are the staple crops in the area.
Specific PCR primers for rapid detection of five rat and mouse species in Java, Indonesia Pramana Yuda; Stephanie Rani Tiurma Siregar; Sena Adi Subrata
Indonesian Journal of Biotechnology Vol 29, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.89125

Abstract

Identifying rat and mouse species quickly, affordably, and accurately is crucial for effective population management, as well as for eradication or conservation purposes. However, the sheer diversity of these species poses a challenge. To address this, a molecular approach has been developed, involving the amplification of a short genetic marker from materials commonly left by the animal, such as hairs and feces. Recent available PCR primers were not suitable for the surveillance of large sample sizes. As a solution, this study designed and validated a PCR primer set capable of detecting five species of rats and mice (Mus musculus, Rattus tanezumi, Bandicota indica, Rattus tiomanicus, and Rattus argentiventer) commonly found in Java, Indonesia. The specific primers were derived from the cytochrome c oxidase subunit 1 (COI) gene, designed using the SP‐Designer V7.0 application, and validated using both in silico and in vitro methods. The validation results demonstrated that all five pairs of primers were highly specific, generated correct amplicons, and successfully detected the five distinct species present in a Javan mongoose feces sample. These findings are significantly important as they enable the effective detection of rat and mouse species and potentially provide valuable ecological insights from the field.
Co-Authors . Subeno Ambar Kusumandari Atus Syahbudin Eko Kosasih Frita Kusuma Wardhani Ikhwanudin Rofi’i Kristiani Fajar Wianti Muhammad Faddel Jauhar Pramana Yuda Stephanie Rani Tiurma Siregar Ulfah Darmayani Permatasari