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All Journal Bioscientist : Jurnal Ilmiah Biologi SEHAT : JURNAL KESEHATAN TERPADU
Intan Kurnia Putri
Program Studi Farmasi, STIKes Mitra Keluarga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Neuroscience Nursing Public Health Social Sciences Veterinary

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Bioprospeksi Ekstrak Etanol Batang Serai Dapur Cymbopogon citratus (DC.) Stapf. sebagai Antibakteri Staphylococcus aureus ATCC : 25923 Reza Anindita; Anna Affifah Ramadhena; Melania Perwitasari; Dede Dwi Nathalia; Maya Uzia Beandrade; Intan Kurnia Putri
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7072

Abstract

Bioprospection of medicinal plants does not only require empirical information, but also requires scientific evidence information. One of the potential medicinal plants that require scientific evidence regarding the ability of antibacterial activity is lemon grass (Cymbopogon citratus). The purpose of this study was to determine the effectiveness of lemongrass in inhibiting the growth of Staphylococcus aureus bacteria. The design of this study was an experiment using ethanol extract samples of lemon grass stems and bacteria S. aureus ATCC : 25923. The research procedures included: determination, preparation, extraction, evaporation, and phytochemical screening of lemon grass stem powder taken from the Pasir Angin area, Bogor, West Java. Treatments in this study included: ethanol extract of lemon grass stems with concentrations of 10%, 20%, 30%, 40%, 50%, 30µg chloramphenicol as positive control, and sterile distilled water as a negative control. All treatments were given 30µL on MHA media containing S. aureus culture. Data analysis in this study was carried out with a quantitative descriptive test. The results showed that the yield percentage of the viscous extract was 18.59% with positive phytochemical screening for alkaloids, tannins, saponins, flavonoids, and terpenoids. The antibacterial test results of the ethanol extract of lemon grass stem 10%, 20%, 30%, 40%, and 50% against S. aureus were able to produce inhibition zone diameters of 1 mm, 1.25 mm, 2 mm, and 2.41 mm, and 3 mm with sensitivity response category resistant. The conclusion in this study was that the administration of ethanol extract of lemon grass stems with 10%, 20%, 30%, 40%, and 50% was not effective in inhibiting the growth of S. aureus so that the lemon grass stems were taken from the Pasir Angin area, Bogor, West Java not recommended as a candidate raw material for pharmaceutical formulations.
UJI EVALUASI DAN AKTIVITAS SALEP EKSTRAK DAUN BINAHONG (ANREDERA CORDIFOLIA), MINYAK ATSIRI LEMON, DAN MINYAK CALENDULA TERHADAP PENYEMBUHAN LUKA SAYAT Anisa Rachmita Arianti; Maya Uzia Beandrade; Intan Kurnia Putri; Dimo Pratama
SEHAT : Jurnal Kesehatan Terpadu Vol. 5 No. 4 (2026)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/s-jkt.v5i4.61673

Abstract

Meningkatnya minat masyarakat terhadap produk perawatan tubuh berbahan alami mendorong pengembangan sabun cair natural yang tidak hanya aman digunakan, tetapi juga memiliki mutu fisik yang baik dan diterima oleh konsumen. Kombinasi minyak atsiri jeruk nipis (Citrus aurantifolia) dan kemangi (Ocimum basilicum) dipilih karena memiliki aktivitas antibakteri, antiseptik, serta memberikan aroma yang menyegarkan. Penelitian ini bertujuan untuk mengevaluasi tingkat penerimaan konsumen terhadap sabun cair natural yang diformulasikan dengan kombinasi kedua minyak atsiri tersebut melalui uji hedonik serta menganalisis stabilitas fisik sediaan selama penyimpanan. Penelitian menggunakan rancangan eksperimental dengan beberapa variasi formula. Penilaian hedonik dilakukan oleh panelis berdasarkan parameter warna, aroma, tekstur, dan tingkat kesukaan secara keseluruhan. Evaluasi stabilitas fisik meliputi pengamatan organoleptik, pengukuran pH, viskositas, tinggi busa, serta uji iritasi kulit. Data hasil pengujian dianalisis menggunakan One Way ANOVA pada tingkat kepercayaan 95% untuk mengetahui perbedaan antarformula. Hasil penelitian diharapkan menunjukkan bahwa formula sabun cair natural dengan kombinasi minyak atsiri jeruk nipis dan kemangi memiliki karakteristik fisik yang stabil, tidak menimbulkan iritasi, serta memperoleh tingkat penerimaan yang baik berdasarkan uji hedonik. Dengan demikian, formulasi yang dikembangkan berpotensi menjadi produk kosmetik berbahan alami yang memenuhi aspek mutu, keamanan, dan preferensi konsumen sehingga layak untuk dikembangkan lebih lanjut sebagai sabun cair natural.
Co-Authors Anisa Rachmita Arianti Anna Affifah Ramadhena Dimo Pratama Dwi Nathalia, Dede Maya Uzia Beandrade Melania Perwitasari Reza Anindita Anindita