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All Journal Nusantara Scientific Medical Research Journal PCJN Pharmaceutical and Clinical Journal of Nusantara

Abdul M Ukratalo

Department of Biology, Faculty of Mathematics and Natural Sciences, Pattimura University, Ambon

Author-ID : 6098485

Dentistry Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Inhibition of Apoptosis of Liver Cells of Mice Infected With Plasmodium berghei Through The Expression of Caspase-3 Using Sargassum duplicatum Extract Abdul M Ukratalo
NSMRJ: Nusantara Scientific Medical Research Journal Vol. 1 No. 1 (2022): NSMRJ: Nusantara Scientific Medical Research Journal
Publisher : Nusantara Scientific Medical

The process of apoptosis is an integrated process between external and internal factors involving several enzymes (Caspase-9, -8, -7, -6, -3) that act as major players in the process of apoptosis. This research aims to determine the potential of brown algae methanol extract Sargassum duplicatum against inhibition of apoptosis of liver cells of mice infected with Plasmodium berghei through the expression of caspase-3. Mice weighing 20–30 grams in Plasmodium berghei infection as much as 0.1 ml per head and left until the percent of parasitemia reaches 1-5%. Then mice (Mus musculus) were given methanol extract of Sargassum duplicatum seaweed at a dose of 1 gr / 100 ml, 10 gr / 100 ml, 100, gr / 100 ml, and 200 gr / 100 ml for 4 consecutive days and observed until day 6. After that, a histological preparation was made with immunohistochemistry staining to see the expression of caspase-3. The results of the observations will be analyzed descriptively. The results showed that Sargassum duplicatum methanol extract was able to inhibit liver cell apoptosis in mice infected with Plasmodium berghei. The decrease in Caspase-3 expression in this study is thought to be caused because the brown algae Sargassum duplicatum contains flavonoid compounds, tannins, and saponins which can reduce the pro-inflammatory cytokine caspase-3 through the role of NF-kB which is a transcription factor that plays a role in stimulating and coordinating innate and adaptive immune responses.

Improved Quality of Spermatozoa Mice (Mus musculus) Model of Diabetes Mellitus Type 1 After Being Given Cinnamon Bark Methanol Extract (Cinnamomum burmanii Bl.) Abdul M Ukratalo; Titus Frendi Wedilen; Ian Risaldy Tofure; Dodikrisno Ekaputra Manery; Muhammad Reza Ramadhany
NSMRJ: Nusantara Scientific Medical Research Journal Vol. 1 No. 1 (2022): NSMRJ: Nusantara Scientific Medical Research Journal
Publisher : Nusantara Scientific Medical

Diabetes mellitus has to do with male fertility, a hormone disorder that affects spermatogenesis. This study aims to determine the effect of cinnamon bark methanol extract (Cinnamomum burmani) on the quality of diabetes mellitus mice spermatozoa. This study used a complete randomized design with 4 treatments and 3 repeats. 22 mice were divided into 4 groups. Mice have injected with streptozotocin dose of 0.1 mL and observed blood sugar levels were, if sugar levels increased, they are given cinnamon bark methanol extract at a dose of 250 mg/kg BB and 500 mg/kg BB, and blood sugar levels are measured. After that, the mice are dissected for observation of spermatozoa's morphology, viability, and motility after administration of the extract. The results showed that cinnamon bark methanol extract at a dose of 250 mg/kg BB and 500 mg/kg BB can reduce the number of abnormal spermatozoa and increase the viability and motility of diabetes mellitus mice spermatozoa. This indicates that cinnamon bark extract as an antioxidant has a positive effect in maintaining structure and development, as well as the function of spermatogenesis cells so that in the presence of these active substances, the number of seed cells that experience developmental failure, degeneration, death due to free radicals can be suppressed or reduced.

Leukocyte Differentiation Mice (Mus Musculus) Infected with Plasmodium Berghei after Therapy With Sargassum Duplicatum Extract Abdul M Ukratalo; Suyono Lateke
NSMRJ: Nusantara Scientific Medical Research Journal Vol. 2 No. 01 (2023): NSMRJ: Nusantara Scientific Medical Research Journal
Publisher : Nusantara Scientific Medical

Leukocytes are white blood cells produced by hematopoietic tissue that serves to help the body fight various infectious diseases as part of the immune system. Sargassum duplicatum contains steroid, alkaloid, phenol, flavonoid, saponin, and tannin compounds that can be immunomodulatory. The purpose of this research is to know the effect of methane extract of brown algae Sargassum duplicatum on the differentiation of leukocyte mice (Mus musculus) infected with Plasmodium berghei. Mice weighing 20-30 grams in Plasmodium berghei infection as much as 0.1 ml per head and left to the percentage of parasitemia reaches 1-5%. Then the mice (Mus musculus) were given brown algae methanol extract (Sargassum duplicatum) at doses 10, 100, and 200 mg / g BW for 4 consecutive days. From day 0 to 6 also made a blood smear to calculate the number of leukocytes. Data analysis will be done using ANOVA. The results showed that the administration of brown algae extracts of Sargassum duplicatum influenced in increasing the number of neutrophils, monocytes, and basophils and decreased the number of eosinophils and lymphocytes of mice infected with Plasmodium berghei to near normal range. Dose 200 mg/kg BB methanol extract of brown alga Sargassum duplicatum is a dose that can affect the number of neutrophils, eosinophils, lymphocytes, and monocytes of mice infected with Plasmodium berghei most closely to normal range.

Potential Bark Cinnamomum burmanii in Regenerating Damaged Liver Cells of Mice (Mus musculus) Diabetes Mellitus Model Abdul M Ukratalo; Martha Kaihena; Dion P O Sirait; Nadira Pattimura; Dodikrisno Ekaputra Manery
PCJN: Pharmaceutical and Clinical Journal of Nusantara Vol. 2 No. 01 (2023): PCJN: Pharmaceutical and Clinical Journal of Nusantara
Publisher : Nusantara Scientific Medical

Diabetes mellitus is a chronic disease that can quickly increase the prevalence of complications in sufferers, such as liver damage. This study aims to examine the effect of methanol extract cinnamon bark (Cinnamomum burmanii Bl) in regenerating liver cell damage mice (Mus musculus) Diabetes Mellitus through the histological picture. 20 mice were divided into 5 groups which consisted of a negative control group, Positive control and a group of mice that were given methanol extract bark Cinnamomum burmannii dose of 125 mg/head/day, 250 mg/head/Day and 500 mg/head/day. In the positive control group and the group of mice that will be given extract streptozotocin injected dose of 0.2 ml and observed blood sugar levels if sugar levels have increased then given methanol extract bark Cinnamomum burmannii with a dose that has been determined and blood sugar levels were measured. On the last day, surgery is performed to remove the liver, after which histological preparations are made. The results showed that the administration of bark extract Cinnamomum burmanii can regenerate damaged liver cells of mice due to diabetes mellitus. This is due to the content of secondary metabolite compounds contained in the bark of Cinnamomum burmannii.

Co-Authors Dion P O Sirait Dodikrisno Ekaputra Manery Ian Risaldy Tofure Martha Kaihena Muhammad Reza Ramadhany Nadira Pattimura Suyono Lateke Titus Frendi Wedilen

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