Jason Theola
Fakultas Kedokteran, Universitas Indonesia, Salemba, Jakarta, Indonesia

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Laporan Kasus Berbasis Bukti: Akurasi Diagnostik RT-PCR Spesimen Saliva terhadap Spesimen Swab Nasofaring pada Suspek COVID-19 Jason Theola; Ananda Maulana Fanshur; Muhammad Rayyan Faher Shahab; Nico Gamalliel
Majalah Kedokteran Indonesia Vol 71 No 6 (2021): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.71.6-2021-414

Abstract

Introduction: Nasopharyngeal swab followed by RT-PCR has become the gold standard in diagnosing COVID-19. This method is difficult to perform because the procedure is inconvenient for the patient. There is another diagnostic method using saliva as an RT-PCR specimen.Objective: To determine the diagnostic accuracy of saliva specimen RT-PCR in diagnosing COVID-19 compared to nasopharyngeal swab specimens as the gold standard.Methods: Literature searching was performed by searching literature through MEDLINE, EBSCO, and Scopus. The articles selected were diagnostic or cross-sectional studies containing sensitivity, specificity, prevalence data, and appropriate to our PICO and eligibility criteria. A critical appraisal was performed after obtaining the selected articles.Results: Six articles were critically appraised. All articles showed that the saliva sample used for RT-PCR in diagnosing COVID-19 had good diagnostic accuracy.Conclusion: Saliva specimen to diagnose COVID-19 can replace nasopharyngeal swab specimen because it has comparable diagnostic accuracy with the gold standard. It is also a non invasive method which is more convenient for patients.
Perbandingan Hasil Biakan Spesimen Respirasi pada Agar Miring dan Cawan Petri (Plate) di Laboratorium Parasitologi Fakultas Kedokteran Universitas Indonesia Jason Theola; Mulyati Mulyati; Anna Rozaliyani; Ridhawati Ridhawati; Robiatul Adawiyah
Majalah Kedokteran Indonesia Vol 72 No 5 (2022): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.72.5-2022-401

Abstract

Introduction: One of the main health problems in Indonesia is respiratory disease. Respiratory disease can be caused by fungus, so-called lung mycosis. The fungi species that cause respiratory infections in humans range from Candida sp. to more dangerous species such as Aspergillus fumigatus. Culture methods using respiratory specimens, especially sputum, can be used to identify species of fungi that cause pulmonary mycosis. Aim this study was conducted to compare the culture results between conventional culture methods on sloped agar and high volume culture method on plate.Method: This study used a cross-sectional design in which the data of culture results were obtained from different culture methods.Result: High volume culture method grew more colonies including Aspergillus fumigatus than conventional culture method which grew no Aspergillus fumigatus. The correlation of data between culture methods and culture results were analyzed with McNemar test and it showed p=0.000.Conclusion: The relationship between the culture method and culture results has a significant correlation (p less than 0.05). Therefore, high volume culture was a better method to grow more fungal colonies including Aspergillus fumigatus than conventional culture methods.