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All Journal Chalaza Journal of Animal Husbandry Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan
Langgeng Priyanto
Universitas Sriwijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Veterinary

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The Effect of Centrifugation Time on Sexing Spermatozoa with Bovine Serum Albumin Media on the Morphology of X-Y Spermatozoa in Simmental Cattle Langgeng Priyanto; R. Riswandi; Oktora Dwi Putranti; Muhammad Gunawan; Apriansyah Susanda; Khusnul Khotimah
Chalaza Journal of Animal Husbandry Vol 7, No 1 (2022): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.126 KB) | DOI: 10.31327/chalaza.v7i1.1706

Abstract

Sexing and centrifugation of spermatozoa is one way to improve the quality of spermatozoa. This study aimed to obtain the right centrifugation time for sexing spermatozoa with Bovine Serum Albumin media on the morphology of X-Y spermatozoa in Simmental cattle. This study was carried out from October 2021 to November 2021 at the Laboratory of Animal Reproduction and Health at the Breeding Center for Superior Livestock and Forage, Sembawa. The design used in this study was a completely randomized design (CRD) with 3 treatments and 4 replications. Centrifugation treatments for sexing spermatozoa with BSA media P1: centrifugation time 4 minutes, P2: centrifugation time 8 minutes, and P3: centrifugation time 12 minutes. The parameters observed were the morphology of the X and Y spermatozoa. The results showed that the centrifugation time for sexing spermatozoa—which was different with Bovine Serum Albumin (BSA) media—indicates that the X spermatozoa abnormalities had no significant effect (P0.05) but the Y spermatozoa abnormalities had a significant effect (P0.05). The result of the right time use was in the P1 treatment with a centrifugation time of 4 minutes where the abnormalities value in the lower layer Y was 28.79%.
Different effects of swamp probiotics application frequency as a biofloc-forming agent on the production of catfish (Clarias gariepinus) Mohamad Amin; Retno Cahya Mukti; Ferdinand Hukama Taqwa; Andini Andini; Marsi Marsi; Langgeng Priyanto; Marini Wijayanti
Depik Vol 13, No 2 (2024): AUGUST 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.2.34280

Abstract

Catfish (Clarias gariepinus) that are reared with probiotics as biofloc-forming agent is thought to increase the fish production. Applying swamp probiotics to the water media has never been studied to ensure the flocks' availability in the rearing media. This study aimed to determine the appropriate frequency of probiotics application collected from swamps for biofloc formation to improve the catfish production. This study used a completely randomized design with two treatments and three replications. The treatments were composed of different application frequency of swamp probiotics: (P1) once in 42 days of rearing and (P2) twice in 42 days of rearing. Data on flock volume, total bacterial colonies, absolute growth rate, feed efficiency, survival rate, and water quality were analyzed by T-test with a 95% confidence level. Meanwhile, the flock composition data were analyzed descriptively. The results showed that P2 obtained the best treatment with a floc volume of 68.33 10.41 mL/L, absolute length growth of 8.18 1.03 cm, absolute weight growth of 19.30 3.12 g, feed efficiency of 135.24 7.98%, survival rate of 89.33 6.21%, biomass production of 24639.50 1344.51 g, temperature of 28.85-29.59C, pH of 7.27-7.42, dissolved oxygen (DO) of 3.91-5.72 mg/L, ammonia of 0.45-1.15 mg/L, and total dissolved solids (TDS) of 717.33-885.50 mg/L. Therefore, swamp probiotics should be applied to catfish culture media twice for 42 days of rearing or once every 21 days.Keywords:BioflocCatfishProbiotics from swamp
Co-Authors Andini Andini Apriansyah Susanda Ferdinand Hukama Taqwa Khusnul Khotimah Marini Wijayanti Marsi Marsi Mohamad Amin Muhammad Gunawan Oktora Dwi Putranti Retno Cahya Mukti Riswandi Riswandi