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All Journal Journal of Tropical Biodiversity and Biotechnology
Nanik Rahmani
Center for Applied Microbiology Research, Research Institute for Life and Environmental Sciences, National Research and Innovation Agency (BRIN), Cibinong, Bogor 16911, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase Rina Dwi Agustiani; Oedjijono Oedjijono; Nanik Rahmani; Nuraeni Ekowati
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.78346

Abstract

The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%
Co-Authors Agustiani, Rina Dwi Nuraeni Ekowati Oedjijono Oedjijono