Isolation and Characterization of Rhizospheric Bacteria Associated with Canna Plant for Production of Maltooligosaccharide Amylase

Rina Dwi Agustiani (Faculty of Biology, University of Jenderal Soedirman, Jalan dr. Soeparno 63 Purwokerto 53122, Indonesia Department of Biology, Faculty of Science and Technology, International Women University, Bandung 40173, Indonesia.)
Oedjijono Oedjijono (Faculty of Biology, University of Jenderal Soedirman, Jalan dr. Soeparno 63 Purwokerto 53122, Indonesia.)
Nanik Rahmani (Center for Applied Microbiology Research, Research Institute for Life and Environmental Sciences, National Research and Innovation Agency (BRIN), Cibinong, Bogor 16911, Indonesia.)
Nuraeni Ekowati (Faculty of Biology, University of Jenderal Soedirman, Jalan dr. Soeparno 63 Purwokerto 53122, Indonesia.)

Journal of Tropical Biodiversity and Biotechnology
Vol 8, No 2 (2023): August

The objectives of the study were to isolate amylolytic bacteria from the rhizosphere and plant tissue of Canna edulis Ker., as well as litter; to know oligosaccharide compounds produced from starch hydrolyzed by the bacterial enzymes, and to identify the amylolytic bacteria based on phenetic and 16S rRNA gene sequences. From the rhizosphere, Canna plant tissue, and litters obtained thirty-two amylolytic bacterial isolates. Eight isolates (TH6, TH7, T5, T10, D2, D3, A3, S1) produced high clear zone diameters ranging from 18-30 mm; especially an isolate T10, which was consistent in producing a total clear zone diameter of 20 mm. The hydrolysate of starch hydrolysed by the T10 amylase resulted in three oligosaccharide compounds maltotriose, maltotetraose, and maltopentose. The amylase activity of isolate T10 was optimal at a temperature of 40°C and pH at 0.801 U/mL. The isolate T10 was identified as a species member of Bacillus toyonensis based on phenotyphic characterization and 16S rDNA gene sequencing analysis with a similarity value of 99.93%