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Roushandy Fardani
Politeknik Medica Farma Husada Mataram
Chemical Engineering, Chemistry & Bioengineering Mathematics Nursing Physics Public Health

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Uji Aktivitas Antibakteri Ekstrak Daun Suruhan (Peperomia Pellucida (L.) Kunth) Terhadap Bakteri Staphyloccocus Epidermidis Roushandy Fardani; Rabulani Apriliani
JSN : Jurnal Sains Natural Vol 1 No 2 (2023): May
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.1 KB) | DOI: 10.35746/jsn.v1i2.339

Abstract

Staphylococcus epidermidis is a gram-positive bacterium that can cause skin infections, one of which is acne. Acne (acne vulgaris) is a chronic inflammatory disease of the pilosebaseus unit characterized by the presence of blackheads, papules, pustules, nodules, cysts, and scars. Acne often occurs on the skin of the face, neck, chest and back. Although acne is not fatal, it is quite troubling because it can reduce self-confidence, especially those who care about appearance. The purpose of this study was to identify the antibacterial activity of suruhan leaf extract (Peperomia pellucida (L.) kunth) against Staphyloccocus epidermidis bacteria. The research method with well diffusion is to see the presence of clear zones formed around wells that show the inhibition of the growth of Staphyloccocus epidermidis bacteria by Peperomia pellucida (L.) kunth leaf extract. The object of study was the extract of uruhan leaves and bacteria Staphyloccocus epidermidis. Data were obtained using primary data from observation or direct observation of the area of inhibition zone of uruhan leaf extract against Staphyloccocus epidermidis bacteria. From the results of the study showed that there was an inhibitory power of the leaf extract against Staphyloccocus epidermidis bacteria.
Uji Aktivitas Antioksidan Ekstrak Bunga Rosella (Hibiscus Sabdariffa) Menggunakan Metode 1,1-difenil-2-pikrilhidrazyl (DPPH) Roushandy Fardani; Rodiatullah Rodiatullah; Idham Halid; Bustanul Atfal
JSN : Jurnal Sains Natural Vol 1 No 4 (2023): November
Publisher : Puslitbang Sekawan Institute Nusa Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35746/jsn.v1i4.411

Abstract

Antioksidan merupakan suatu senyawa yang dibutuhkan oleh tubuh untuk menangkal radikal bebas antioksidan ini dapat menghambat terjadinya reaksi oksidasi, dengan mengikat radikal bebas dan molekul yang sangat reaktif. Salah satu senyawa yang dapat menangkap radikal bebas yaitu turunan Flavonoid. Tanaman bunga rosella mengandung senyawa flavonoid yaitu golongan antosianin. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antioksidan alami ekstrak bunga rosella dengan metode DPPH (1,1-Diphenyl-2-Picrylhidrazyl). Pengukuran absorbansi dari kandungan flavonoid yang terdapat pada bunga rosella menggunakan psektrofotometri Uv-Vis. Uji aktivitas antioksidan dengan metode DPPH dan parameter nilai IC50, untuk menghitung aktivitas antioksdian menggunakan persamaan regresi linear. Hasil aktivitas antioksidan dari bunga rosella yaitu 1.01 μg/mL dan aktivitas antioksidan dari vitamin C sebagai pembanding yaitu 43.29 μg/mL. Aktivitas antioksidan pada bunga rosella masuk kedalam kategori sangat kuat dan untuk vitamin C termasuk kedalam kategori sangat kuat.
Co-Authors Bustanul Atfal Idham Halid Rabulani Apriliani Rodiatullah Rodiatullah