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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Muhammad Hipzul Mursyid
University of Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Isolation of Cellulolytic Bacteria from Kalkun (Meleagris gallopavo) Gastro-Intestinal Tract as a Candidate Probiotics for Poultry Muh. Aidil Fitriyan Fadjar Suryadi; Muhammad Hipzul Mursyid; Khairil Anwar; Muhamad Ali; Djoko Kisworo
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3739

Abstract

This study has been conducted to isolate and identify cellulolytic bacteria from the digestive tract of turkeys (Meleagris gallopavo) as probiotic candidates for poultry. This study has obtained 4 bacteria that have the potential as probiotic candidates through the morphological identification stage, the ability to produce cellulase enzymes and resistance to acidic pH conditions. The test found that RDL code bacteria bacteria have the highest ability to produce enzyme cellulase characterized by the formation of a clearing zone with a diameter of 11 mm, the second highest PVB2 code bacteria with a clearing zone diameter of 7 mm, DB1 4 mm, and RSL 2 mm and the results of bacterial viability tests isolated at low pH (2.0) showed the highest viability after incubating for 4 hours. RDL viability is 65%, RSL 48%, DB1 50%, and PVb2 29%. So based on the tests that have been carried out, it is estimated that the 4 bacteria are candidates for Lactobacillus sp. worthy to be used as a probiotic for poultry
Isolation and Identification of Swan (Cygnus olor) Digestive Tract Cellulothic Bacteria to Support Fiber Degradation Muhammad Subhan Bahruddin Rosyidi; Muhammad Ali; Wayan Wariata; Djoko Kisworo; Khairunnisah; Qothrunnada Amira Salma; Muhammad Hipzul Mursyid
Jurnal Penelitian Pendidikan IPA Vol 10 No SpecialIssue (2024): Science Education, Ecotourism, Health Science
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10iSpecialIssue.8516

Abstract

Cellulolytic bacteria are bacteria that produce cellulase enzymes capable of degrading cellulose substrates. This study aimed to isolate and identify as well as measure the cellulase enzyme activity of cellulolytic bacteria isolated from the digestive tract of swans (Cygnus olor) in Mataram. The bacteria were isolated using de Man Rogosa Sharpe (MRS) selective media with the spread plate method. Then, the extracellular enzyme activity test was conducted by growing selected pure isolates on 1% Carboxy Methyl Cellulose (CMC 1%) media, followed by pouring 0.1% congo red solution and rinsing with 1M NaCl solution to determine cellulolytic activity (cellulolytic potential indicated by the appearance of a clear zone around the colony) and measuring the cellulolytic index. The bacterial isolation results obtained 4 isolates with the potential to support fiber degradation in animal feed. The highest cellulolytic index was produced by the isolate coded S1, reaching 34.5 mm, while the lowest cellulolytic index was produced by 2 isolates coded J21 and I2, reaching 18.8 mm.
Co-Authors Djoko Kisworo Djoko Kisworo Khairil Anwar Khairunnisah Muh. Aidil Fitriyan Fadjar Suryadi Muhamad Ali Muhammad Ali Muhammad Subhan Bahruddin Rosyidi Qothrunnada Amira Salma Wayan Wariata