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All Journal Jurnal Biogenerasi Spizaetus: Jurnal Biologi dan Pendidikan Biologi BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Pharmaceutical and Sciences. BIO-CONS: Jurnal Biologi dan Konservasi
Leni Widiarti, Leni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Energy Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary Other

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PENGARUH PEMBERIAN EKSTRAK ETANOL BUAH AREN (Arenga pinnata Merr.) TERHADAP KADAR KREATININ, UREUM DAN HISTOLOGI GINJAL TIKUS PUTIH (Rattus norvegicus L.) YANG DIINDUKSI KARBON TETRAKLORIDA (CCl4) Awaliah Sifa, Nurharija; Febriani, Husnarika; Widiarti, Leni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5637

Abstract

One substance that produces radical compounds is carbon tetrachloride (CCl4). As a solvent, insecticide, coolant, stain remover, and soap, carbon tetrachloride is widely used. Direct skin contact, ingestion, inhalation, and eye contact are ways this material enters the body. The antioxidants found in palm fruit (Arenga pinnata Merr.) can help protect against damage caused by free radicals. This study aims to determine how giving ethanol extract of palm fruit (Arenga pinnata Merr.) to white rats (Rattus norvegicus L.) affects creatinine, urea levels and kidney histology when exposed to carbon tetrachloride (CCl4).This study used a completely randomized design with 20 mice divided into 5 treatment groups. Negative controls were given food, drink and 1 ml/kg BW olive oil, positive controls were given 1 ml/kg BW carbon tetrachloride and olive oil with a ratio of 1:3. Treatments 1, 2 and 3 were given carbon tetrachloride 1ml/kg BW and olive oil with a ratio of 1:3 and given ethanol extract of palm fruit at different doses (P1= 90 mg/kg BW, P2= 180 mg/kg BW, P3= 360 mg/kg BW) for 16 days. The results obtained include that ethanol extract of palm fruit has a very significant effect on creatinine, urea and kidney histology. The optimal dose of palm fruit ethanol extract to improve kidney is a 360 mg/kg BW.
Pengaruh Pemberian Ekstrak Daun Salam (Syzygium polyanthum) terhadap Ginjal Tikus Putih (Rattus norvegicus L.) yang Diinduksi Kadmium Klorida (CdCl2) Rismayani, Fanya Audi; Syukriah, Syukriah; Widiarti, Leni
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 1 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i1.2218

Abstract

Kadmium merupakan logam berat yang jika terpapar secara berlebihan dapat menyebabkan nefroksisitas yang ditandai dengan penurunan fungsi ginjal akibat stres oksidatif yang dipicu oleh radikal bebas. Daun salam diketahui mengandung senyawa antioksidan seperti flavonoid dan tanin yang berperan dalam menangkal radikal bebas. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun salam (Syzygium polyanthum) terhadap morfologi, indeks organ ginjal, kadar kreatinin dan kadar ureum ginjal pada tikus yang diinduksi CdCl2. Penelitian ini menggunakan RAL dengan 20 ekor tikus dibagi menjadi 5 kelompok perlakuan, Kontrol Negatif : makan dan minum, Kontrol Positif : CdCl2 40 mg/kg BB, Perlakuan 1 : CdCl2 40 mg/kg BB + ekstrak daun salam 200 mg/kg BB, Perlakuan 2 : CdCl2 40 mg/kg BB + ekstrak daun salam 300 mg/kg BB dan Perlakuan 3 CdCl2 40 mg/kg BB + ekstrak daun salam 400 mg/kg BB. Tahapan dalam penelitian ini meliputi skrining fitokimia, uji aktivitas antioksidan, pengamatan morfologi, perhitungan indeks berat organ pemeriksaan kadar kreatinin dan ureum ginjal tikus putih. Analisis data menggunakan uji one way ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian yang didapatkan diantaranya, daun salam tidak berpengaruh terhadap morfologi ginjal, namun daun salam berpengaruh dalam menurunkan rata-rata indeks organ ginjal dengan yaitu pada dosis ekstrak 300 mg/kg BB, serta berpengaruh dalam menurunkan kadar kreatinin dan ureum pada tikus putih yang diinduksi kadmium klorida dengan dosis ekstrak daun salam yang optimal yaitu pada dosis 400 mg/kg BB.
PENGARUH PEMBERIAN EKSTRAK ETANOL BUAH AREN (Arenga pinnata Merr.) TERHADAP KADAR KREATININ, UREUM DAN HISTOLOGI GINJAL TIKUS PUTIH (Rattus norvegicus L.) YANG DIINDUKSI KARBON TETRAKLORIDA (CCl4) Awaliah Sifa, Nurharija; Febriani, Husnarika; Widiarti, Leni
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5637

Abstract

One substance that produces radical compounds is carbon tetrachloride (CCl4). As a solvent, insecticide, coolant, stain remover, and soap, carbon tetrachloride is widely used. Direct skin contact, ingestion, inhalation, and eye contact are ways this material enters the body. The antioxidants found in palm fruit (Arenga pinnata Merr.) can help protect against damage caused by free radicals. This study aims to determine how giving ethanol extract of palm fruit (Arenga pinnata Merr.) to white rats (Rattus norvegicus L.) affects creatinine, urea levels and kidney histology when exposed to carbon tetrachloride (CCl4).This study used a completely randomized design with 20 mice divided into 5 treatment groups. Negative controls were given food, drink and 1 ml/kg BW olive oil, positive controls were given 1 ml/kg BW carbon tetrachloride and olive oil with a ratio of 1:3. Treatments 1, 2 and 3 were given carbon tetrachloride 1ml/kg BW and olive oil with a ratio of 1:3 and given ethanol extract of palm fruit at different doses (P1= 90 mg/kg BW, P2= 180 mg/kg BW, P3= 360 mg/kg BW) for 16 days. The results obtained include that ethanol extract of palm fruit has a very significant effect on creatinine, urea and kidney histology. The optimal dose of palm fruit ethanol extract to improve kidney is a 360 mg/kg BW.
Pemberian Pupuk Kompos dan Cair Azolla terhadap Pertumbuhan dan Kandungan Flavonoid Tanaman Kedelai Hitam (Glycine max L.) Hariani, Widia; Rahmadina, Rahmadina; Widiarti, Leni
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.545

Abstract

Black soybean (Glycine max L.) is a food crop commodity. The low productivity of soybean in Indonesia is caused by several factors, including the availability of low-quality seeds and the unavailability of nutrients. Therefore, this study aims to determine the effect of compost and liquid Azolla fertilizer (Azolla pinnata) on the growth and flavonoid content of black soybean plants (Glycine max L.). With the factorial RAK method. The first factor is without Azolla compost fertilizer (K0), Azolla compost fertilizer 250 gr / polybag (K1), Azolla compost fertilizer 300 gr / polybag (K2). The second factor is without liquid azolla fertilizer (C0), azolla liquid fertilizer 130 ml / l of water (C1), azolla liquid fertilizer 150 ml / l of water (C2). And the data is processed using SPSS with the ANOVA test. If the treatment obtained has a very significant effect, it will be continued with the DMRT test. The results showed that Azolla compost fertilizer affected plant height, number of leaves and total leaf area with a dose of 250 gr/polybag. While Azolla liquid fertilizer did not affect all parameters at all doses, namely 130 and 150 gr/polybag. The highest flavonoid content in black soybean leaves was found in the 250gr compost fertilizer treatment, namely 99.72247 QE/g. It can be concluded that Azolla compost fertilizer is more effective in increasing the flavonoid content of black soybean leaves. The flavonoid content of the 250gr compost fertilizer treatment was 99.72247 QE/g in the leaves.
Analisa Kromatografi Gas Spektrometri Massa dan Gugus Fungsi Ekstrak Metanol Daun Bidara (Ziziphus mauritiana): Analisa Kromatografi Gas Spektrometri Massa dan Gugus Fungsi Ekstrak Metanol Daun Bidara (Ziziphus mauritiana) Widiarti, Leni; Febriani, Husnarika; Dur, Sajaratud; Ningrum, Nurlian Augustin; Nurcahyani, Nadya; Andry, Muhammad
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.449

Abstract

Bidara leaves are a plant that is quite abundant in Medan and has many benefits in the health sector. The aim of this research is to predict the content of secondary metabolite compounds in bidara leaf extract, through qualitative tests by screening using specific reagents, functional group analysis with FT-IR and mass analysis with GC-MS. The screening results showed that the methanol extract of bidara leaves contained alkaloids, flavonoids, tannins, saponins and phenols. Functional group analysis shows that the wavelength area is 3300 cm-1, namely OH absorption, 2700 - 2900 cm-1 shows C-H absorption, 1600 - 1700 cm-1 shows C=O absorption, 1400 - 1450 cm-1 shows C-H absorption, 1365 - 1385 cm-1 shows CH3 bent absorption, and 1000 – 1100 cm-1 shows C-O absorption. The results of mass analysis using GC-MS showed four dominant peaks from the compound, namely Z-5-Methyl-6-heneicosen-11-one, 17-Pentatriacontene, Ethyl iso-allocholate and Pentacosane.
Pengaruh Konsentrasi dari Gliserol Terhadap Plastik Biodegradable Berbahan Pati dari Kulit Singkong Widiarti, Leni; Dur , Sajaratud; Siregar , Aisyah Rafiqah Azla
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 4 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i4.1072

Abstract

Indonesia is the second-largest producer of plastic waste in the world, while conventional plastics require a long time to decompose and may generate harmful emissions. Therefore, the development of biodegradable bioplastics has become increasingly important. Abundant cassava peels have potential as a starch source, with glycerol serving as a plasticizer and chitosan as a reinforcing agent. This study aimed to analyze the effect of varying glycerol concentrations (0, 2, 4, 6, and 8 mL) on the functional group characteristics and thermal properties of cassava peel starch–chitosan-based bioplastics. The bioplastics were synthesized by blending starch and chitosan at an 8:2 ratio, followed by the addition of glycerol at different concentrations. Characterization was conducted using Fourier Transform Infrared (FTIR) spectroscopy and Differential Scanning Calorimetry (DSC). FTIR results indicated no formation of new functional groups; however, shifts and broadening of the –OH absorption bands were observed, suggesting physical interactions through hydrogen bonding among starch, chitosan, and glycerol. DSC analysis revealed that glycerol concentration significantly affected the thermal properties of the bioplastics, with the highest melting temperature (Tm) obtained at 6 mL glycerol (160.67 °C) and the lowest at 8 mL glycerol (129.33 °C). The formulation containing 8 mL glycerol exhibited the highest fusion enthalpy (321.73 J/g). These findings indicate that the addition of 6 mL glycerol provides the most optimal plasticization condition with the highest thermal stability, whereas higher glycerol concentrations result in over-plasticization. The optimal formulation shows potential for further development as an environmentally friendly packaging material.
Penurunan Kadar Tembaga (Cu) dengan Biostimulasi Glukosa pada Bakteri Indigenous dari Sungai Bedera Kecamatan Medan Marelan, Kota Medan, Sumatera Utara Lubis, Resti Ayunda; Nasution, Rizki Amelia; Widiarti, Leni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.18072

Abstract

Sungai Bedera adalah sungai yang tergolong tercemar logam berat akibat pembuangan limbah rumah tangga dan industri yang memiliki kadar logam berat tembaga (Cu) yang tinggi yaitu 0,6518 mg/L. Cara yang dilakukan untuk menurunkan kadar logam berat tembaga (Cu) adalah dengan memanfaatkan bakteri Indigenous. Penelitian ini bertujuan untuk mengidentifikasi bakteri indigenous dari Sungai Bedera dan menguji kemampuannya dalam menurunkan kadar Cu. Bakteri Indigenous diberi perlakuan dengan nutrisi tambahan yaitu biostimulan glukosa. Metode yang dilakukan yaitu isolasi bakteri indigenous, uji resistensi bakteri dengan menghitung nilai Optical Density (OD) menggunakan spektrofotometer Uv-Vis dan proses penurunan kadar logam berat (Cu) dilakukan dengan metode SSA (Spektrofotometer Serapan Atom). Hasil uji menggunakan One Way ANOVA menunjukkan adanya perbedaan nyata antarperlakuan (Sig. < 0,05). Empat genus bakteri indigenous yang berhasil diidentifikasi memiliki kemampuan menurunkan kadar logam berat tembaga (Cu) yaitu Enterococcus, Bacillus, Corynebacterium, dan Staphylococcus. Persentase penurunan Cu tanpa biostimulan glukosa pada masing-masing bakteri Enterococcus, Bacillus, Corynebacterium dan Staphylococcus adalah 51,49%, 40,65%, 29,39%, dan 20,70%. Persentase penurunan Cu dengan penambahan biostimulan glukosa pada masing-masing bakteri Bacillus, Enterococcus, Staphylococcus dan Corynebacterium adalah (77,60%), (72,28%), (66,66%), dan (60,63%). Bakteri indigenous yang paling tinggi dalam menurunkan kadar tembaga (Cu) dengan penambahan biostimulan glukosa adalah Bacillus dengan persentase penurunan 77,60% dan bakteri indigenous yang paling rendah dalam menurunkan kadar tembaga (Cu) tanpa penambahan biostimulan glukosa adalah Staphylococcus dengan persentase penurunan 20,70%. Kata kunci : Bakteri Indigenous, Biostimulan, Logam Berat Tembaga (Cu), dan Sungai Bedera
Co-Authors Awaliah Sifa, Nurharija Dur , Sajaratud Hariani, Widia Husnarika Febriani, Husnarika Lubis, Resti Ayunda Muhammad Andry Nasution, Rizki Amelia Ningrum, Nurlian Augustin Nurcahyani, Nadya Rahmadina, Rahmadina Rismayani, Fanya Audi Sajaratud Dur, Sajaratud Siregar , Aisyah Rafiqah Azla Syukriah Syukriah, Syukriah