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Efektivitas Larutan Dekalsifikasi pada Os tibia Domba Garut (Ovis aries) (THE EFFECTIVENESS OF DECALCIFYING SOLUTIONS ON THE TIBIAL OF GARUT SHEEP (OVIS ARIES)) Handina Rakhmawati; Adrian Situmeang; Nurhidayat Nurhidayat; Andri Maruli Tua Lubis; Harry Murti; Arief Boediono
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Bone is a tissue that has a density of extracellular matrix structures and composed by organic and inorganic components. Decalcification is a stage that plays an important role in bone histology using various types of solutions. The sample used in this study was lateral condyle from tibia of three garut sheeps (Ovis aries) which had been fixed with 10% Neutral Buffered Formalin (NBF) for 24 hours. Sample were cut into pieces ranging from 1 cm x 1 cm x 1 cm in size, the decalcification using three solutions; 10% nitric acid, 10% EDTA (pH 7.4) and 10% EDTA (pH 7.4) + TBD-1®. The aim of this study was to evaluate the effectiveness of three solutions for the decalcification process of lateral tibial condyle of garut sheep. Observation parameter in this study includes: duration of decalcification, sectioning process of ribboning formation, structural integrity and absorption of hematoxylin-eosin (HE) staining. The results shows that 10% EDTA (pH 7.4) solution provides a long duration of decalcification which is ease sectioning process ribboning, the best structural integrity of lateral tibial condyle. In hematoxylin-eosin (HE) staining shows that 10% nitric acid solution does not absorb the optimum color, opposite in 10% EDTA and 10% EDTA + TBD-1® solution, the color intensity between hematoxylin and eosin in the tissue shows the best results. Based on these results, it can be concluded that the 10% EDTA (pH 7.4) is the best decalcification solution for lateral condyle from tibial of garut sheeps.

Efek Regional dan Cardiopulmonary Penggunaan Lidocaine dan Lidocaine-Xylazine pada Blokade Nervus Ischiadicus Domba (REGIONAL AND CARDIOPULMONARY EFFECTS OF LIDOCAINE AND LIDOCAINEXYLAZINE IN SHEEP SCIATIC NERVE BLOCK) Herawati Napitu; Deni Noviana; Raden Harry Soehartono; Nurhidayat Nurhidayat
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

General anaesthesia has a high risk during surgery, regional anaesthesia with nerve block is expected to minimize the use of general anaesthesia while increase the analgesic effect and muscle relaxation effect. This study aimed to compare the analgesic effects of sciatic nerve block on the hind limb and the effect of lidocaine 2%, and a combination of 2% lidocaine with 2% xylazine on sheep cardiopulmonary function. Sciatic nerve block was guided with ultrasonography and nerve stimulator. In this research, sheep were sedated with diazepam (0.5 mg / kg). Sheep was divided into three groups; first injected with saline solution (SS), second with lidocaine 2% 5 mg / kg (Lid) and third with lidocaine 2% 5 mg / kg and xylazine 2% 0.1 mg / kg (LidXyl). The results of this research showed that the LidXyl group had the highest analgesic effect and broader blockade area, namely femoris, cruris, and metatarsus-digit. The LidXyl blockade value was highest in the digitmetatarsus region, while the Lid group shows the highest blockade value in the cruris region. The LidXyl group with the addition of xylazine results in a decrease in heart rate and respiration rate. Xylazine increased and extended analgesic effect compared to lidocaine but resulted in a decrease in heart rate and respiration rate in the early minutes of anaesthesia, it is however safe to be used in sheep.

ANATOMIALAT PENCERNAAN IKAN BUNTAL PISANG (Tetraodon lunaris) [Anatomy of The Digestive Apparatus of The Puffer Fish Tetraodon lunaris] Yusfiati Yusfiati; Koeswinarning Sigit; Ridwan Affandi; Nurhidayat Nurhidayat
Jurnal Iktiologi Indonesia Vol 6 No 1 (2006): Juni 2006
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v6i1.231

This research was aimed to study not only on gross morphologically but also on the histological structure of the Tetraodon lunaris digestive apparatus. The fish samples were obtained from a fisherman at the fish auction in Pelabuhan Ratu, from August 2005 until March 2006. The ratio between intestine and length/body length represented that this fish is carnivorous. The esophagus is short and the gastric forms a simple pouch with two of small diverticula. The intestine consists of a single loop. The esophagus and gastric mucous layer is covered with epithelial stratified cells. The intestine mucous layer has covered with a layer of columnar epithelial cells. The esophagus and gastric muscular layer consist of striated muscle. The sphincter pylorica and the intestine muscular layer consist of smooth muscle. The anus mucous layer consits of epithelial cuboid and it has the muscular layer of striated muscles. The liver has oval-shaped greenish vesica fellea with ductus choledochus emptied at the anterior part of intestine. The hepatocyt cells have a polygonal shape with lipid droplets in its. The exocrine glands of the pancreas tissue showed a set of acinar cells in the liver parencyhm, around portal vein, hepatic artery and biliary ductules.

Development of Adrenocorticotrophic Hormone Cells of Adenohypophysis–Adrenal Cortex Axis of Long-tailed Monkey (Macaca fascicularis) during Pre and Postnatal Period Nurhidayat Nurhidayat; Ika K. Syarifah; Supratikno Supratikno; Sri Wahyuni
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

The objective of this study is to observe the relationship of development of adrenocorticotropic hormone (ACTH) cells of adenohypophysis–adrenal cortex axis during pre and postnatal period of longtailed monkey (Macaca fascicularis). Five fetuses aged 70, 85, 100, 120, and 150 days of gestation and 2new born aged 10 and 105 days old were used as research materials. The sections of the pituitary gland stained immunohistochemically using human ACTH antiserum and the adrenal glands stained by Hematoxylin-Eosin procedures. At the fetus 120 days old, the ACTH cells and blood vessel of fetaladenohypophysis developed well and its similar that found in older age. This condition suggested distributing the ACTH to whole body optimally. At the same time, the adrenal cortex cells of fetus 120 days old were active in proliferation and differentiation processes to form transition zone that lies between definitive and fetal zones, and central vein also developed in adrenal gland. In fetus aged 150 days, the cortex adrenal structure was similar with found in older fetus. The adenohypophysis ACTH cells developed earlier than adrenal cortex and their secretion suggested influencing the development of adrenal cortex cells of long-tailed monkey.

ANALISIS POTENSI DAUN TORBANGUN (Plectranthus amboinicus) SEBAGAI ANTIBAKTERI TERHADAP Klebsiella pneumoniae SECARA IN SILICO Nabila Nur Nafiati; Rini Madyastuti Purwono; Nurhidayat Nurhidayat; Laksmi Ambarsari
Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Vol 8 No 1 (2023): Pharma Xplore : Jurnal Sains dan Ilmu Farmasi
Publisher : Fakultas Farmasi Universitas Buana Perjuangan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36805/jpx.v8i1.5190

Antibiotic-resistant is a global issue. Klebsiella pneumoniae has high priority to find its antibiotic because it has extended-spectrum beta-lactamase (ESBL) enzyme that makes the bacteria easy to resist the antibiotic. The study of phytochemicals in medicinal plants is one of the efforts to find antibiotics. Indrulizia is one of the empirical medicinal plants containing flavonoids used to treat disease. The aim of this study was to analyze the potential of borage as an antibacterial agent against Klebsiella pneumoniae in silico. Docking is the method for this study using AutoDock Vina. This is an application for docking the enzyme ESBL from K. pneumoniae, referred to as SHV (sulfhydryl variable), and the metabolite second from plants. The parameters analyzed were binding free energies, inhibition constants and visualization in 3D and 2D. The free energy of binding and the inhibition constant of the native ligand are -6.7 kcal/mol and 12.118 μM. The binding free energy values of apigenin 7-glucuronide and rosmarinic acid show higher potencies than the native ligand of SHV-1 (1,4-thiazepine).

Development of Adrenocorticotrophic Hormone Cells of Adenohypophysis–Adrenal Cortex Axis of Long-tailed Monkey (Macaca fascicularis) during Pre and Postnatal Period Nurhidayat Nurhidayat; Ika K. Syarifah; Supratikno Supratikno; Sri Wahyuni
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9783

The objective of this study is to observe the relationship of development of adrenocorticotropic hormone (ACTH) cells of adenohypophysis–adrenal cortex axis during pre and postnatal period of longtailed monkey (Macaca fascicularis). Five fetuses aged 70, 85, 100, 120, and 150 days of gestation and 2new born aged 10 and 105 days old were used as research materials. The sections of the pituitary gland stained immunohistochemically using human ACTH antiserum and the adrenal glands stained by Hematoxylin-Eosin procedures. At the fetus 120 days old, the ACTH cells and blood vessel of fetaladenohypophysis developed well and its similar that found in older age. This condition suggested distributing the ACTH to whole body optimally. At the same time, the adrenal cortex cells of fetus 120 days old were active in proliferation and differentiation processes to form transition zone that lies between definitive and fetal zones, and central vein also developed in adrenal gland. In fetus aged 150 days, the cortex adrenal structure was similar with found in older fetus. The adenohypophysis ACTH cells developed earlier than adrenal cortex and their secretion suggested influencing the development of adrenal cortex cells of long-tailed monkey.

Karakteristik Morfofungsi Skelet Ekstremitas Kaki Soa Layar (Hydrosaurus amboinensis) Yanri R.N. Simangunsong; Savitri Novelina; Supratikno Supratikno; Danang C. Cahyadi; Chairun Nisaâ€™; Heru Setijanto; Srihadi Agungpriyono; Nurhidayat Nurhidayat
Jurnal Biologi Papua Vol 15 No 1 (2023)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.2575

The Sailfin lizard is a lizard that has a semi-aquatic life and has a sail fin on its tail. These lizards can be found in habitats close to water such as rivers, lakes, and estuaries of mangrove forests. This study aimed to observe the morphofunctional characteristic of the appendicular skeleton of Sailfin Lizard (Hydrosaurus amboinensis), associated with their function and behavior. The appendicular skeleton of the Sailfin lizard was studied morphofunctional by observing and measuring the bones that make up the extremities. The naming of bones and their parts is based on the Nomina Anatomica Veterinaria 2017 and other journals. The clavicle and interclavicle bones are located anterior to the scapula coracoid which has a convex shape. The patella ulnar bone is found at the humeroulnar joint and the radius et ulna bones have a relatively long antebrachial interosseous space. On the hind legs, the coxal bone has a relatively broad acetabulum, two lunula bones and patellar mineralization are found at the craniodorsal of the femorotibiofibular joint, and tibia et fibula bones have a relatively long crural interosseous space. The palmar part of the carpal bone is found in the palmar sesamoid bone, while the tarsi bones have a fused astragalus and calcaneus bones to become astragalocalcaneus bones. The pedis skeleton's size is relatively long compared to the manus skeleton and has a different middle phalanx bone. The extremity skeletal structure allows the Sailfin Lizard to do movements such as walking, running, climbing, running on water, and swimming.Key words: appendicular skeleton; morphofunction; Sailfin lizard.

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