Supratikno .
Bagian Anatomi, Histologi, Dan Embriologi, Departemen Anatomi, Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor.

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Morfofisiologi dan Profil Biokimia Darah Kalong Kapauk (Pteropus vampyrus) dari Wilayah Pesisir Kabupaten Garut Danang Dwi Cahyadi; . Nurhidayat; Chairun Nisa'; . Supratikno; Savitri Novelina; Heru Setijanto
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.316 KB) | DOI: 10.29244/avi.6.1.51-59

Abstract

Aktivitas terbang pada kelelawar membutuhkan energi paling banyak dibandingkan dengan aktivitas lokomosi lainnya. Morfofisiologi dan profil biokimia darah diduga memiliki peranan penting terhadap kemampuan terbang hewan tersebut. Penelitian ini bertujuan untuk mengetahui karakteristik morfofisiologi eritrosit dan profil biokimia darah P. vampyrus. Penelitian ini menggunakan 15 ekor kelelawar dewasa dengan bobot badan antara 669,7 g sampai 1211,5 g (x̄ = 957,51 ± 177,52 g). Sampel darah diambil secara langsung melalui ventrikel kiri jantung. Pengamatan dan pengukuran terhadap preparat ulas darah menunjukkan bahwa morfologi eritrosit P. vampyrus mirip dengan mamalia secara umum dan mempunyai diameter rata-rata 7,15 ± 0,45 µm. Koefisien variasi ukuran eritrosit (RDWc) hewan ini sebesar 18,11 ± 1,16%.  Pemeriksaan hematologi yang dilakukan menggunakan automated counter menunjukkan bahwa total eritrosit (8,89 ± 1,36 106/µl),  konsentrasi hemoglobin (14,33 ± 2,38 g/dl), dan nilai hematokrit (42,13 ± 6,49%) P. vampyrus relatif lebih tinggi dibandingkan dengan mamalia pada umumnya. Neutrofil dan limfosit merupakan komponen yang mendominasi jumlah leukosit. Adapun persentase jumlah neutrofil lebih tinggi dibandingkan dengan jumlah limfosit. Penelitian ini memberikan informasi dasar yang dapat mendukung penelitian terkait dengan kemampuan terbang dari P. vampyrus.
PF-7 Pre Slaughter Stunning of Ruminant Slaughter Based on MUI HAS 23103 2012, MS 1500 2009 and SNI 99003 2018 . Supratikno; Danang Dwi Cahyadi; Heru Setijanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

The debate about the use of pre slaughter stunning (PSS) in halal slaughter is still an interesting topic to discuss due to the difficulties to determine the stunned animal are unconscious or dead. Indonesia and Malaysia as a country with majority Muslim population have recognized non-penetrative PSS (NPPSS) as a method in halal slaughter. Indonesian Council of Ulama (MUI) issued the Halal Assurance System (HAS 23103 2012)[1] as guidelines for animal slaughter process, while Department of Standard  Malaysia issued  Malaysian Standard (MS) 1500 2009[2], which regulate Halal Food-Production, preparation Handling and Storage-General Guidelines. Recently, the Indonesian National Standardization Body issued Indonesia's National Standard for ruminant halal slaughter (SNI 99003 2018) [3]. This article will discuss mechanical NPPSS based on these three guidelines in animal welfare and halal perspective 
PF-30 Anatomical Characteristic of Hindlimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis) . Nurhidayat; Eni Puji Lestari; Danang Dwi Cahyadi; Chairun Nisa'; . Supratikno; Savitri Novelina; Heru Setijanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Sumatran rhino (Dicerorhinus sumatrensis) is an endangered animal in Indonesia. Currently, Sumatran rhino only found on Sumatera and Kalimantan in very small populations. These herbivorous animals are classified into the order of Perissodactyla (odd-toed animals) and family Rhinocerotidae [1]. Sumatran rhino is one of the largest living land mammals, reaching 1000 kg [1], although this species has the smallest body weight among all extant rhinos. This animal has a round and long body shape, relatively short legs with three digits on each leg. These body structures correspond to their habitat in the highlands, so the Sumatran rhino has an excellent ability to pass steep terrain [2]. For this reason, a strong hind limb structure is needed to push the body when walking, running and climbing the steep slopes. Therefore, this study was carried out in order to analyze the correlation between the Sumatran rhino’s hind limb skeleton and its functional roles.
AH-02 Cutaneous Muscle of Javan Porcupines (Hystrix javanica) Supratikno Supratikno; Halim Bakti Harjo; Danang Dwi Cahyadi; Srihadi Agungpriyono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Cutaneous Muscle of Javan Porcupines (Hystrix  javanica)
AH-03 Anatomical Characteristic of Forelimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis) Nurhidayat Nurhidayat; Eni Puji Lestari; Chairun Nisa'; Danang Dwi Cahyadi; Supratikno Supratikno
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Anatomical Characteristic of Forelimb Skeleton of Sumatran Rhino        (Dicerorhinus sumatrensis)
AH-04 Anatomical Characteristic of the Pelvic and Thigh Region Muscles of Javan Pangolin, Manis javanica Chairun Nisa'; Singgih Pratiknyo Sundawa; Supratikno Supratikno; Danang Dwi Cahyadi
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Anatomical Characteristic of the Pelvic and Thigh Region Muscles  of Javan Pangolin, Manis javanica
Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) I Ketut Mudite Adnyane; Supratikno .; Adi Winarto; Srihadi Agungpriyono
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
INDENTIFIKASI TINGKAT KESEHATAN DAN STRES KELELAWAR PEMAKAN BUAH (Cynopterus tithaecheilus) SEBAGAI MAMALIA TERBANG Anisa Rahma; Desrayni Hanadhita; Andhika Yudha Prawira; Danang Dwi Cahyadi; Supratikno Supratikno; Hera Maheshwari; Aryani Sismin Satyaningtijas; Srihadi Agungpriyono
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.937 KB) | DOI: 10.19087/jveteriner.2018.19.4.460

Abstract

Hematology value is important to evaluate individual health status. This study was purposed to observe the hematological values of male and female Cynopterus titthaecheilus. The study was conducted on 16 C. titthaecheilus (six adult males, six adult females and four juveniles) to study the haematological profiles. Blood samples were taken using tuberculin needles and analyzed by hematology analyzer for hematological values. Females C. titthaecheilus had a higher number of white blood cells than the males, but no differences in the white blood cells differential. Males C. titthaecheilus had a higher number of platelets than the females. Generally, the juveniles had a higher number of leucocytes as well as number of platelets than the adults. Based on the stress index value, it was concluded that young bats had a lower stress level compared to adult bats.
Bleeding Time and False Aneurysm Incidence on Cattle Slaughtering Using Non-Penetrative Pre-Slaughter Stunning in Indonesia Supratikno; H. Setijanto; H. Nuraini; S. Agungpriyono
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.482

Abstract

The use of non-penetrating pre-slaughter stunning (NPPSS) in ruminants remains a contentious issue in Indonesia since it is thought to interfere with the bleeding. This paper evaluated the bleeding time (BT), incidence of the false aneurysm (FA), and factors influencing these parameters in 460 NPPSS and 50 non-NPPSS slaughters in Indonesia. In this research, bodyweight, neck skin fold, sex, incisors condition, stunner strength, door closed-final stunning interval, number of shots, stunning success rate (SSR), stunning final-slaughter interval, broken skull, shooting placement, stunner-men and slaughter-men competencies, incision direction, incision location, BT, and FA were assessed for NPPSS slaughter. The same parameters were also assessed for non-NPPSS except the stunning parameter. The results showed that the BT of the non-NPPSS slaughters (187±47.37 seconds) was faster than that of the NPPSS slaughters, with the BT for the NPPSS dependent upon the level of unconsciousness. The longest BT was found in fully unconscious cattle shot once (304.32±69.76 seconds). The risk of FA incidence in non-NPPSS cattle was higher than in the NPPSS cattle. The FA incidence was 2.64 times higher in non-NPPSS cattle than in unconscious NPPSS cattle. The BT of non-NPPSS cattle was affected by FA, whereas the NPPSS cattle were affected by SSR, number of shots, broken skull, and FA incidence. The primary influence factor of FA in non-NPPSS cattle was incision location, whereas the NPPSS cattle were DCFS, incision location, and incision direction. Based on the results of this study, it can be concluded that the use of NPPSS prolongs BT and reduces the risk of FA in the slaughter of Australian Brahman cross cattle.
CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE Andhika Yudha Prawira; Desrayni Hanadhita; Anisa Rahma; Supratikno Supratikno; Savitri Novelina; Srihadi Agung Priyono
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (743.751 KB) | DOI: 10.17969/rtp.v%vi%i.6892

Abstract

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.