Neneng Syarifah Syafei, Neneng Syarifah
Departemen Mikrobiologi dan Parasitologi, Universitas Padjadjaran

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Bacterial and Antibiotic Susceptibility Patterns in Patient with Pediatric Urinary Tract Infection at Dr. Hasan Sadikin General Hospital Muttaqin, Muhammad Ilham; Hilmanto, Dany; Syafei, Neneng Syarifah
Althea Medical Journal Vol 4, No 1 (2017)
Publisher : Althea Medical Journal

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Abstract

Background: Pediatric urinary tract infection (UTI) should become attention due to high prevalence, severe complication, and high cost treatment. Bacterial pattern and its susceptibility are different in every region.The objective of this study was to discover pattern of bacteria causing UTI and their susceptibility to antibiotics. Methods: This study was an observational descriptive study. The data was obtained from medical records and susceptibility testing results of pediatric UTI in Department of Child Health at Dr. Hasan Sadikin General Hospital in period of 2014. Data taken was presented in table.Results: There were 26 cultures which had microbiological findings among 79 cultures taken from 106 patients with UTI. The majority of findings were Escherichia coli (10 cases), Pseudomonas aeruginosa (4 cases), and Klebsiella pneumoniae (4 cases). Antibiotic classes with high susceptibility level were aminoglycoside, sulfonamide, and fluoroquinolone. Resistance level were higher in cephalosporin class than others.Conclusions: Most common bacterial found are Escherichia coli, Pseudomonas aeruginosa, and Klebsiellapneumoniae. The result of susceptibility testing for cultures shows variance of susceptibility pattern to antibiotics in pediatric patients with UTI at Department of Child Health Dr. HasanSadikin General Hospital. [AMJ.2017;4(1):52–7] DOI: 10.15850/amj.v4n1.1021 
Gambaran Kontainer Potensial dan Kondisi Lingkungannya Sebagai Tempat Perindukan Nyamuk di Universitas Padjadjaran Jatinangor Faridah, Lia; Hamda, Muhammad Ersyad; Syafei, Neneng Syarifah; Agrianfanny, Yukan Niko
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.967 KB) | DOI: 10.15395/mkb.v50n2.1151

Abstract

Nyamuk memiliki peran penting sebagai vektor penyakit menular seperti demam berdarah dengue, malaria, filariasis, demam kuning, dan chikungunya sehingga keberadaan nyamuk perlu dikontrol. Pengawasan tempat perindukan nyamuk akan membantu pihak berwenang untuk merancang pengendalian kepadatan nyamuk. Pertumbuhan larva nyamuk dipengaruhi oleh kondisi lingkungan seperti suhu air, kelembapan udara, dan pH air. Kondisi tersebut dapat bervariasi karena perbedaan geografis, variasi musiman, atau bahkan perubahan iklim.Tujuan penelitian ini adalah mengetahui kontainer potensial dan kondisi lingkungannya sebagai tempat perindukan nyamuk di Universitas Padjadjaran Jatinangor. Penelitian deskriptif dilakukan dengan mengamati larva nyamuk dalam wadah air baik di dalam maupun di luar gedung fakultas. Kondisi lingkungan (pH, suhu dan kelembapan) diukur dan dicatat selama pengamatan untuk setiap kontainer. Hasil penelitian menunjukkan bahwa dari 582 kontainer yang diteliti terdapat 72 (12,4%) positif  larva nyamuk. Kontainer yang paling potensial di dalam bangunan adalah ember, sedangkan di luar bangunan adalah bambu Aedes sp. Mendominasi penemuan larva di lapangan. Suhu air rerata yang diperoleh adalah 24,3oC, kelembapan 66,7% dan pH 8,1. Kondisi suhu dan pH sesuai dengan kondisi optimum perkembangan larva pada umumnya. Sementara tingkat kelembapan yang lebih rendah (kelembapan 81,6–89,5%) masih mampu membuat larva nyamuk tumbuh dengan baik di Universitas Padjadjaran Jatinangor.Kata kunci: Kelembapan, larva nyamuk, pH, suhu air, Universitas Padjadjaran Potential Container and Its Environmental Conditions for Mosquito Breeding Site in Universitas Padjadjaran JatinangorPlaying pivotal role as vector of infectious disease such as dengue hemorrhagic fever, malaria, filariasis, yellow fever and chikungunya, mosquito needs to be controlled. Surveillance for mosquito breeding places will help the authorities in devising means in controlling mosquito density. The growth of mosquito larvae is influenced by environmental conditions such as water temperature, humidity, and pH. Those conditions may vary due to geographic differences, seasonal variations, or even climate change. The purpose of this study was to understand the potential container and its environmental conditions for larvae in Universitas Padjadjaran Jatinangor. A descriptive study was conducted by observing the presence of mosquito larvae in water containers both inside and outside of faculty buildings. Environmental conditions (pH, water temperature and humidity) were measured and recorded during observation for each water container.  Results showed that from 582 containers examined, 72 (12.4%) were positive for larvae. The most potential container in the building was bucket, while foroutside of the building, the most potential container was bamboo. Aedes sp. dominated larvae discovered in this study. The average water temperature obtained was 24.30C with 66% humidity, and pH 8.1. These temperature and pH values are the optimum condition for larvae development in general.  However, lower humidity level (humidity 81.6–89.5%) can still enable good growth of mosquito’s eggs in the campus area of Universitas Padjadjaran Jatinangor.Key words: Humidity, mosquito larvae, pH, water temperature, Universitas Padjadjaran 
Perbandingan Efektifitas Antara Minyak Atsiri Kulit Batang Kayu Manis (Cinnamomum burmannii) dengan Temephos sebagai Larvasida Aedes aegypti Al Kamal, Muhamad Rizki; Syafei, Neneng Syarifah; Nasution, Gita Tiara Dewi
Pharmaceutical Sciences and Research Vol. 4, No. 1
Publisher : UI Scholars Hub

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Abstract

Dengue fever is a major health issue in the world. Preventive measure with temephos/abate has long been used to combat this problem. Istiana et al reported that there were cases of resistance of Aedes aegypti larvae to temephos in various parts of the world, including Indonesia. Indonesian Cinnamon (Cinnamomum burmannii) has been proven to possess larvacide effect and can be used as an alternative insecticide. This research was meant to compare the larvacide effect of Indonesian cinnamon (Cinnamomum burmannii) essential oil to the larvacide effect of temephos against Aedes aegypti larvae. This research used laboratory experimental design analysis. The total number of samples were 20 larvae of Aedes aegypti at 3rd and 4th instar which were tested four times on each four experimental groups. The collected data were the total number of Aedes aegypti larva deaths observed 24 hours after the variables were applied. The analysis of the results was done by using statistic analysis program. There were significant differences on larva deaths due to the essential oil and abate variables with p value < 0.001. The results were as follows: negative control death (0%), emulsifier (2.5%), essential oil (22.5%), and abate (100%). The larvacide effectiveness of abate is significantly better than that of Indonesian cinnamon essential oil.
Perbandingan Efek Larvasida Minyak Atsiri Daun Cengkeh (Syzygium aromaticum L.) Varietas Zanzibar dengan Temephos terhadap Larva Nyamuk Aedes aegypti Pamungkas, Ridzmullah Wishnu; Syafei, Neneng Syarifah; Soeroto, Arto Yuwono
Pharmaceutical Sciences and Research Vol. 3, No. 3
Publisher : UI Scholars Hub

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Abstract

The use of temephos as larvicides in disease prevention Dengue Hemorrhagic Fever (DHF) in Indonesia has been going on for 40 years. Larviciding very long led the resistance to temephos. Cloves, one of native Indonesia plant contains eugenol that are toxic to the insect’s body. This study aims to examine the effectiveness of Zanzibar clove leaf essential oil compared to temephos in killing the larvae of Aedes aegypti. This study uses experimental laboratory research design. The samples are larvae of Aedes aegypti stage III. The mosquito larvae were classified into six groups, each given a clove leaf essential oil with different concentrations. Next will be observed the number of larvae that die after 24 hours. Based on statistical test p-value p < 0.05 it indicates there are differences in the average mortality in the test group, and post hoc analysis showed the test group with 100 ppm of essential oil is not a significant difference with temephos. This study suggests the essential oil of clove leaf varieties of Zanzibar has the same effectiveness with temephos in killing the larvae of Aedes aegypti.