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All Journal Proceeding Biology Education Conference BIOVALENTIA: Biological Research Journal
Sri Pertiwi Estuningsih, Sri Pertiwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Asosiasi Konsorsium Bakteri Pseudomonas Pseudoalcaligenes dan Micrococus Luteus dengan Lamtoro (Leucaena Leucocephala (Lamk.) De Wit) dalam Upaya Meningkatkan Bioremediasi Minyak Bumi Marsandi, Fenky; Estuningsih, Sri Pertiwi
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.706 KB)

Abstract

Penelitian mengenai Asosiasi Konsorsium Bakteri Pseudomonas pseudoalcaligenes dan Micrococus luteus dengan Lamtoro (Leucaena leucocephala (Lamk.) de Wit) Dalam Upaya Meningkatkan Bioremediasi Minyak Bumi telah dilakukan pada bulan Januari-Maret 2012 di area jurusan Biologi, Laboratorium Mikrobiologi Jurusan Biologi dan Laboratorium Kimia Analisa Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sriwijaya. Penelitian ini bertujuan untuk mengkaji kerja sama antara tanaman Leucaena leucocephala dengan konsorsium bakteri Pseudomonas pseudoalcaligenes dan Micrococus luteus dalam proses bioremediasi minyak bumi dengan menghitung penurunan nilai TPH, jumlah sel bakteri dan pertumbuhan tanaman. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 6 kali ulangan. Dimana penanaman lamtoro dilakukan setelah 4 minggu inokulasi bakteri. Variabel yang diamati yaitu penurunan nilai TPH, jumlah populasi bakteri dan pertumbuhan tanaman. Hasil yang didapat, menunjukkan bahwa perlakuan B3 (inokulasi bakteri Micrococus luteus dan Pseudomonas pseudoalcaligenes dan penanaman lamtoro) menghasilkan rata-rata jumlah sel bakteri tertinggi setiap minggunya, pertambahan berat basah tertinggi mencapai 9,67 ± 2,357 dan penurunan rata-rata nilai TPH tertinggi yaitu sebesar 2,85%. Sedangkan perlakuan yang terendah pada perhitungan rata-rata jumlah sel bakteri terdapat pada B0 (bioreaktor) dan  pada pertambahan berat basah tanaman , B1 (lamtoro saja) memiliki nilai yang terendah yaitu 1,55%. Konsorsium bakteri Micrococus luteus dan Pseudomonas pseudoalcaligenes menunjukkan kerja sama yang baik dengan lamtoro dalam upaya meningkatkan bioremediasi minyak bumi.Keywords:        Asosiasi,  Bakteri, Lamtoro, Bioremidiasi
HORMESIS PHENOMENA OF SOME PARAMETER ASPECT IN USAGE OF WATER LETTUCE (Pistia stratiotes L.) FOR PHYTOREMEDIATION PROCESS OF PETROLEUM LIQUID WASTE Marisa, Hanifa; Estuningsih, Sri Pertiwi
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.753 KB) | DOI: 10.24233/BIOV.3.1.2017.59

Abstract

The research about potential test of water lettuce (Pistia stratiotes) in order of phytoremediation of petroleum liquid waste had been done during June untill December 2012 at Microbiology Laboratory, Department of Biology, Faculty of Science, The University of Sriwijaya, Indonesia. Completely Randomized Design was used in the experiment while the concentration of liquid waste as treatment were: 0, 15, 30, 45, 60 and 75 %. Each treatment were replicated 4 times. Three parameter was measured; Total Petroleum Hydrocarbons (TPH) reduction percentage, efficiency of phytoremediation and number of tillers. Regression analisys were used for the data and two of parameters show the hormesis phenomena, were; efficency of phytoremediation and number of tillers. The highest  peak effect of treatment for efficiency of phytoremediation and number of tillers was on 45 %. TPH reduction percentage was the only one parameter that shows linier regression Keywords hormesis, Pistia stratiotes, TPH, phytoremediation, tiller
SETTINGS OF TEMPERATURE AND TIME SAVING ON SEED GERMINATION OF Magnolia champaca (L.) Baill. ex Pierre Nuradinda, Odetta Maudy; Estuningsih, Sri Pertiwi; Harmida, Harmida
BIOVALENTIA: Biological Research Journal Vol. 4 No. 1 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.648 KB) | DOI: 10.24233/BIOV.4.1.2018.86

Abstract

Setting storage space temperature and time saving becomes an important application in supporting the development of plantations to provide seeds as needed and sustainable. The purpose of this research is to delay the rapid of seed germination in Magnolia champaca by assessing the effect of various suspend temperatures and times saving on seed germination of Magnolia camphaca . The research was conducted in Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University in February until June 2016. The method was used completely randomized design pattern Factorial by 3 factors with 12 treatments and 3 replications with the temperature: temperature ± 31°C, temperature ± 25 °C, temperature ± 18 °C, temperature ± 4 °C and the retention of time 0 week, 2 weeks , 4 weeks and 6 weeks. The parameters include the observation of germination rate, germination, vigor index and sprout morphology. The results of the research the treatment of interaction of temperature and time saving significantly affected on germination rate, germination and seed vigor index of Magnolia champaca. Germination speed, germination and vigor index were both produced by the treatment of storage at a temperature of ± 18 °C and a temperature of ± 25 °C at all times to keep (2 weeks, 4 weeks, 6 weeks) compared to control treatment. A normal sprout produced in temperature treatment ± 18 °C and ± 25 °C temperature ranges between 90-93%. An abnormal sprout produced in control treatment (0 week) by 25%. Measurement of moisture content and physiological maturity level of Magnolia champaca seed needs to be done before it is stored and germinated to test the viability of seeds of sprouts.
Co-Authors Hanifa Marisa Harmida Harmida Marsandi, Fenky Nuradinda, Odetta Maudy Nuradinda, Odetta Maudy