Rianida Taisa
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APLIKASI PUPUK KALSIUM DAN LIQUID ORGANIC BIOFERTILIZER TERHADAP PERTUMBUHAN DAN HASIL TANAMAN TOMAT Ratih Rahhutami; Raida Kartina; Rianida Taisa; Wika Anrya Darma; Ferziana Ferziana
Agros Journal of Agriculture Science Vol 25, No 3 (2023): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i3.3077

Abstract

The quality of soil and the availability of nutrients an important role in increasing the gowth and yield of tomato plants. The purpose of this study were to determine the effect of calcium fertilizer, LOB and the interaction between the two as well as to obtain the best concentration of calcium, LOB and a combination of both on the gowth and yield of tomato plants. This study used factorial complete goup randomized design. The first factor was the concentration of calcium: 0 g / l (Ca0) and 2 g / l (Ca1). The second factor was the concentration of LOB: 0 ml/l (P0), 5 ml/l (P1), 10 ml/l (P2), 15 ml/l (P3), and 20 ml/l (P4). The data obtained were analyzed variously at the level of 5%, if there was a significant effect followed by the DMRT test. The results showed that the application of calcium 2 g / l has not been able to increase the gowth and yield of tomato plants and the higher the concentration of LOB given the gowth and yield of tomato plants will increase. The best combination was shown in the treatment of 0 g/l Ca and 15 ml/l LOB. INTISARI Kualitas tanah dan ketersediaan unsur hara berperan penting dalam peningkatan pertumbuhan dan hasil tanaman tomat. Tujuan dari penelitian ini adalah mengetahui pengaruh pupuk kalsium, LOB dan interaksi antara keduanya serta untuk mendapatkan konsentrasi terbaik dari kalsium, LOB dan kombinasi keduanya terhadap pertumbuhan dan hasil tanaman tomat.Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktorial. Faktor pertama adalah konsentrasi kalsium: 0 g/l pupuk kalsium (Ca0) dan  2 g/l pupuk kalsium (Ca1). Faktor kedua adalah konsentrasi LOB: 0 ml/l LOB (P0), 5 ml/l LOB (P1), 10 ml/l LOB (P2), 15 ml/l LOB (P3), dan 20 ml/l LOB (P4). Data yang diperoleh dianalisis ragam pada taraf 5%, jika terdapat pengaruh nyata dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa aplikasi kalsium 2 g/l belum mampu meningkatkan pertumbuhan dan hasil tanaman tomat dan semakin tinggi konsentrasi LOB yang diberikan pertumbuhan dan hasil tanaman tomat akan semakin meningkat. Kombinasi terbaik ditunjukkan pada perlakuan 0 g/l Ca dan 15 ml/l LOB.
IN VITRO PLANT REGENERATION FROM CHRYSANTHEMUM CALLUS INDUCED BY GAMMA IRRADIATION AND ISSR GENETIC ANALYSIS Desi Maulida, Dr.; Rianida Taisa; Lisa Erfa; Nanang Wahyu Prajaka; Dilla Febria; Rahmadyah Hamiranti; Syariful Mubarok
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.11828

Abstract

Conventional breeding methods of chrysanthemum plants face several challenges to overcome, and physical mutagens such as gamma rays have proven effective in creating chrysanthemum mutants. As an important tool in flower germplasm innovation and variety improvement, gamma rays have greater penetrating power compared to other breeding methods. This research aims to determine the effect of various doses of gamma irradiation on chrysanthemum callus growth, plant regeneration, and morphology due to induction and to determine the existence of genetic variations using ISSR markers. The experiment consisted of four stages, namely, chrysanthemum callus explants were irradiated at doses of 0, 10, 20, and 30 Gray using gamma rays to induce mutations and observe the effect on callus growth. The irradiation results were planted in BA media 0.5, 1.5, and 3.0 mg L-1 to observe shoot growth. Next, the shoots were planted in rooting media containing NAA with and without activated charcoal (AC) for plant regeneration and amplification of chrysanthemum genomic DNA. The results showed that a lower radiation dose (10 Gray) had a positive influence on all growth parameters, while 30 Gray caused a significant reduction in plant height and number of leaves. NAA 1 mg L-1 AC 2 mg L-1 media gave the best results in supporting the growth of chrysanthemum plants at various levels of radiation exposure. Analysis using ISSR markers confirmed the presence of genetic variation in irradiated plants.