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PERBAIKAN MUTU GENETIK UDANG GALAH (Macrobrachium rosenbergii) BERDASARKAN SELEKSI FAMILI [Genetic Improvement of Giant Freshwater Prawn (Macrobrachium rosenbergii) based on Family Selection] Hadie, Lies Emmawati; Hadie, Wartono
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.694 KB) | DOI: 10.14203/beritabiologi.v11i2.491

Abstract

The base populations were composed to support selective breeding of giant freshwater prawn (Macrobrachium rosenbergii). Composite population were improved additive and dominance of genetic variance, especially for character of economic important. Economic character of giant freshwater prawn have a ratio of carapace length to standard length (ratio CL/SL).This research is aimed to improved the genetic of carapace and standard length ratio for giant freshwater prawn based on family selection. Selection methods were conducted on trait of carapace and standard length ratio as an edible portion. The base population prawn were used from three location i.e. Cimanuk (Cape of Air, West Java), Citanduy (Pamarican,West Java) and Musi River (Palembang, South Sumatra). Family selection was used selectedstructure. Parents were selected based on breeding value. Natural spawning was used product to first generation (F1) production of population. Larval rearing was used clear water system, fingerling production in the concrete tank, and juvenile rearing was conducted on earthen ponds. Respons selection was estimated to five months of freshwater prawn. Result of this experiment indicated that population of giant freshwater prawn can build by breeding program with heritability value of dressing out to 0.56+0.07; selection differential to 13.74 and selection intensity to 4.05. Prediction of genetic improvement from that genetic parameters is a value 7.69% to one generation. Implementation of population would be increased of genetic quality and there is decreased of gen degradation to prawn population.
PENGGUNAAN Chaetoceros calcitrans, Thalassiosira weissflogii DAN KOMBINASINYA PADA PEMELIHARAAN LARVA UDANG VANAME (Litopenaeus vannamei, Boone 1931) [The Use of Chaetoceros calcitrans, Thalassiosira weissflogii and Its Combination to The Larval Rearing of Vaname (Litopenaeus vannamei, Boone 1931)] Panjaitan, Amyda Suryati; Hadie, Wartono; Harijati, Sri
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.496 KB) | DOI: 10.14203/beritabiologi.v14i3.1826

Abstract

The use of one type live food in the larval rearing of vannamei shrimp is insufficient for maximum larval development. This research was aimed to evaluate the use of phytoplankton Chaetoceros calcitrans and Thalassiosira weissflogii and its combination as food to the growth and survivorship of white pacific shrimp Litopenaeus vannamei. The research used prawn larvae at stadia Nauplius4-5 with 150/ litre larval density. The larvae were fed and their effects with 3 kinds of live food, C. calcitrans (A), T. weissflogii (B), and combination of both types (C) for each treatment with five replications.The data was analysed using SPSSV.16. Result showed that the survival rate for treatment A was of 55.04+11.81%, treatment B was of 68.22+6.80%, and treatment C was of 77.04+4.63%. This indicated that treatment A gave significantly different on survival rate (P<0.01) than treatment B and C. Treatment B and C were not significantly different (P>0.05). We recomended the use of combination both of C. calcitrans and T. weisflogii to provide maximum survival rate for vannamei shrimp postlarvae.
PENGGUNAAN VAKSIN Aeromonas hydrophila: PENGARUHNYATERHADAP SIANTAN DAN IMUNITAS LARVA IKAN PATIN (Pangasionodon hypophthalmus) Lusiastuti, Angela Mariana; Hadie, Wartono
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.062 KB) | DOI: 10.14203/beritabiologi.v10i2.1967

Abstract

Aeromonas hydrophila is a pathogen that often causes considerable losses in the area of freshwater fish fanning. Vaccination is one way to simulate parent catfish make specific immunity. Specific immunity generated by the parent will be forwarded through the oocytes produced during a certain time span. The aim of this research was to know the effect and the effectivity of using hydrovac vaccine with and without the complete adjuvant. This research was done on Patin fish Pangasionodon hypophthalmus whose givng Hydrovac 0.4 ml/kg of body weight. The comparation between complete adjuvant and vaccine was 1:1. Injection was done by intra peritoneal for three mothers each with and without complete adjuvant. Injection was done at gonad maturity level II. The result showed that antibody were positively detected on mother serum which used adjuvant or not. On larva stage, antibody was detected until four weeks old. While on 2 weeks old of larva, the concentration of titer antibody was very high and raised the dilution of 1: 2048. Survival rate of juvenile which their mother got a vaccine raised 93%, was better than 73%-63% using mother without vaccine. Booster immersion of hydrovac vaccine could give preferably at the end of three weeks old or in the beginning of fourth weeks old of larva.
Reproductive Characteristics of Female Egg-carrying Buntingi, Xenopoecilus oophorus, an Endemic Fish to Lake Poso in Central Sulawesi Gundo, Meria Tirsa; Rahardjo, Muhammad Fadjar; Batu, Djamar Tumpal Frederik Lumban; Hadie, Wartono
Makara Journal of Science Vol. 20, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Reproduction characteristic of female egg-carrying buntingi, Xenopoecilus oophorus had been studied. This research was conducted at Lake Poso, Central Sulawesi, Indonesia. Specimens were collected monthly from August 2012 to July 2013 at four sampling stations around the lake. Macroscopic observations of ovarian maturity level and gonadosomatic index revealed a long reproductive period during the rainy season, with four spawning peaks in November, January, February and April. The highest total fecundity was 135 oocytes, and the highest batch fecundity was 36 oocytes. Analysis of the oocyte diameter frequency distribution showed X. oophorus is a multiple spawner. Batch fecundity was correlated (r = 0.78) with body weight.