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All Journal Animal Production : Indonesian Journal of Animal Production
M Indradji, M
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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In Vitro and in vivo Test of Exctract of Morinda citrifolia and Allium sativum on Subclinical Mastistis Cows Sufiriyanto, Sufiriyanto; Indradji, M
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Two subsequent experiments were conducted in the current study. Experiment I (in vitro), milk obtained from sub-clinical mastitis dairy cows were used to identify the content of bacteria types causing mastitis. It was identified that the milk contained Staphylococcus sp (76.92%), Streptococcus sp (15.38%) and E. coli (7.69%). In order to determine the optimum combination of Morinda citrifolia (M) and Allium sativum (A) for reducing the number of the identified bacteria. The milk were randomly assigned to one of four treatments with six replicates in each treatment (Completely Randomized Designed). There were four treatments, which were four kinds of Morinda citrifolia (M) to Allium sativum (A) ratio, i.e. T1 (80:20); T2 (60:40); T3 (40:60); and T4 (20:40). Collected data were analysed using analysis of variance. When analysis of variance indicated significant effects, Least significant different (LSD) was then employed.  It is indicated that T1 was the most optimum treatment to reduce bacteria counts. Experiment II (in vivo), the purpose of the experiment was to detemine the optimum dose of T1 to cure sub-clinical mastitis dairy cows and to investigate the effects of different doses of T1 on milk quality.  A total of 20 sub-clinical mastitis dairy cows were randomly treated with one of five treatments for 3 days, which were C (received penicillin), P1 (received T1:100 ml/d); P2 (received T1:200 ml/d); P3 (received T1:300 ml/d) and P4 (received T1:400 ml/d). Analysis for variance was applied followed by dunnet’s test. Milk production and somatic cell counts were not significantly (P>0.05) affected by the treatments, but they significantly (P<0.005) influenced milk dry matter content. Dairy cows treated with P4 produced milk with the highest dry matter content (11.92 ± 1.90%). In conclusion, The mixtures of  Morinda citrifolia and Allium sativum can cure mastitis and improve milk dry matter content. (Animal Production 7(2): 101-105 (2005) Key Words: Mastitis, Dairy cattle, Allium sativum, Morinda citrifolia, Quality of milk
Evaluation of the Effectiveness of Avian Influenza Vaccines in Native Chicken Using Two by Two Contingency Table Indradji, M; Sufiriyanto, .
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. The aim of this study was to evaluate the effectiveness of vaccination program analysis method using the 2 x 2 contingency table to provide better assessment to the vaccination program management and implementation. This study used survey methods. A total of 230 serum samples from vaccinated chickens and 20 serum samples from unvaccinated chickens were used. The blood serum samples were then examined with the Hemagglutination Inhibition Test/HI to measure antibody levels. The data were analyzed using a 2 x 2 contingency table. Results showed that the level of vaccine protection was 68.92% with 31.31% vaccines failure rate, the level of natural protective immunity in samples was 0%,  100% vaccines specificity and the effectiveness of the vaccine was 71.20%. Keywords: Vaccination, contingency table, hemaglutination inhibition. Abstrak. Penelitian bertujuan untuk mengevaluasi keberhasilan program vaksinasi dengan menggunakan tabel kontingensi 2 x 2 yang diharapkan dapat digunakan untuk menilai manajemen program vaksinasi yang lebih komprehensif. Penelitian ini menggunakan metode survei, dengan total 230 sampel serum dari ayam divaksinasi dan 20 sampel serum dari ayam yang tidak divaksinasi. Sampel serum darah ayam kemudian diperiksa dengan uji hemaglutinasi inhibisi/HI untuk mengukur tingkat antibodi. Data dianalisis dengan menggunakan tabel kontingensi 2 x 2. Hasil menunjukan bahwa tingkat proteksi vaksin yang diaplikasikan sebesar 68,92 % dengan  tingkat kegagalan vaksin 31,31 %. Tingkat kekebalan alami yang protektif pada sampel  sebesar 0 %, spesifitas vaksin 100 %, dan efektifitas  vaksin sebesar 71,20 %. Kata kunci: Vaksinasi, tabel kontingensi, inhibisi hemaglutinasi.M Indradji and Sufirianto/Animal Production 15(1):62-68, January 2013 
Effectiveness of Temulawak (Curcuma xanthoriza) and Kunyit (Curcumae domestica) Extracts to Enhance Productivity and as Immunostimulator of Avian Influenza in Broiler Sufiriyanto, Sufiriyanto; Indradji, M
ANIMAL PRODUCTION Vol 9, No 3 (2007): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The objective of the experiment was to investigate the effectiveness of treating broiler with temulawak (Curcuma xanthoriza) and kunyit (Curcumae domestica) extracts to enhance productivity and as imunostimulator of avian influenza. Broilers were given either temulawak, kunyit or temulawak+kunyit extracts. The treatments, including a control, were arranged in a factorial design. Variables measured were production index and immune titter with haemaglutination inhibition (HI) test at 35 days of age. Results showed that control, temulawak-, kunyit- and temulawak+kunyit-treated chicken have production indexes of 302.80, 382.30, 327.71, and 358.30, respectively. HI test results were all negative. It can be concluded that neither temulawak, kunyit nor temulawak+kunyit extracts is effective imunostimulator of avian influenza in broiler. Nevertheless, temulawak-treated chicken showed highest production index.  (Animal Production 9(2): 178-183 (2007) Key Words: Avian influenza, haemaglutination inhibition, temulawak, kunyit
Evaluation of Inhibition Steroid Effect by Diazinon Pollutions on Ovarium and Cortex Adrenal Glands of the Animal Model Sufiriyanto, Sufiriyanto; Indradji, M
ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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A study was conducted to influence of the diazinon pollutions effect to histopathologic change examination of ovarium and cortex adrenal glands the rat (Rattus norvegicus) caused of steroid inhibition metabolism. The treatment consisted of four level doses of diazinon as follows 0, 50, 100 and 200 ppm for 40 days, in drinking water. The microscopic examination result showed that vacuolar degeneration change in surrounding corpus atreticum and hemorrhagic lesion on interstitial cell of ovarian, in starting dose 50 ppm and then, the swollen cells and congestive lesion of cortex adrenal glands, in starting dose 100 ppm. (Animal Production 4(2): 101-111 (2002) Key words : Diazinon, Steroid, Cortex Adrenal Glands
Contamination Rate of Lead in Pigeon (Columbia livia) at Semarang City Indradji, M; Yuwono, E; Santosa, EB
ANIMAL PRODUCTION Vol 2, No 2 (2000): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The aim of this research was to find out the lead concentration in some pigeon tissues in Semarang city. Fifty four pigeons 4-8 weeks old, which collected from 4 locations in Semarang were devided into 4 groups. Group I (15 pigeons) were obtained from the bird markets, group II (15 pigeons) were obtained from who sale fried birds, and group III (14 pigeons) were collected from the bird owners who look after the birds extensively (not in cages), then group IV (10 pigeons) were obtained from the bird owners who look after the birds intensively (in cages). All pigeons were euthanasia by chloroform then necropsied. A part of tissues eg: muscle, lung, liver and kidney were collected for lead examination by using Atomic Absorpsion Spectrophotometer (AAS). The result of the experiment showed that muscle, lung, liver and kidney pigeons were contaminated by lead, and lead concentration of kidney were higher (P< 0.05) than 0,1 ppm. Location of pigeons were not significant (P>0.05) with lead concentration in tissues pigeons. (Animal Production 2(2): 69-74 (2000) Key words : lead, pigeon, Semarang city
Grassing as potential factor in Gastrointestinal Nematodiasis in Goat in Banyumas District Indradji, M; Yuwono, Endro
ANIMAL PRODUCTION Vol 6, No 2 (2004): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Goat system manajemen like housing, feeding dan grassing have been studied on the diseases association in gastrointestinal nematodiasis infection. The method applied was survey on 150 goat (Jawarandhu) faeces sample in Banyumas District. The Research was carried out from July to September 2004. The result showed that  the nematode infection 1,54 times to goat if grassing and 88,62% infection in groups. Housing, Feeding have no risk and associated with disease infection. (Animal Production 6(2): 95-100 (2004) Key words : management system, disease association, nematode
Co-Authors . Sufiriyanto, . E Yuwono, E EB Santosa, EB Endro Yuwono Sufiriyanto Sufiriyanto, Sufiriyanto