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All Journal Biology, Medicine, & Natural Product Chemistry JSMARTech : Journal of Smart Bioprospecting and Technology
Nafisa Nafisa
Universitas Islam Malang
Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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In Vitro Screening of Antibacterial Activity of Endophytic Mold Extract from Mango Mistletoe Leaves (Dendrophthoe pentandra (L.) miq) Nafisa Nafisa; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.59

Abstract

Endophytic microbes live in plant tissues and can be extracted to obtain active compounds. One conserved endophytic microbes is endophytic mold, found in plant tissue systems, such as leaves, flowers, branches, and plant roots. The endophytic mold was obtained from the mango parasite (Dendrophthoe pentandra (L.) Miq), a parasitic plant that contains secondary metabolites. This study aimed to determine the antibacterial properties of the endophytic mold isolates extract from mango parasite leaves against Escherichia coli. This research used an experimental method with descriptive quantitative data analysis. The research began with the purification of endophytic mold isolates, production of bioactive compounds, extraction of bioactive compounds, and determination of antibacterial activity by disc diffusion method. The results obtained from this study included four isolates containing antibacterial compounds, including isolates DBM 1, DBM 2, DBM 3, and DBM 4. Extracts of the endophytic mold of mango parasite leaves showed quite good inhibition of the growth of Escherichia coli, with the results of measuring the diameter of the largest inhibition zone of 13.17 mm, which was produced by isolate DBM 1 at a concentration of 75 ppm. Based on the results of antibacterial activity tests on endophytic molds from the leaves of the mango parasite (Dendrophtoe pentandra (L.) Miq), isolates of endophytic fungi can be used as antibacterial agents in the medium renewable category.
Antioxidant Activity Analysis Using DPPH and Total Phenol Methods of Soursop Leaf Extract (Annona muricata L.) Dwi Kartika Wati; Kammal Nizam Amrullah; Salsabilatul Mukarommah; Nour Athiroh Abdoes Sjakoer; Faisal Faisal; Majida Ramadhan; Nafisa Nafisa
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.325-330

Abstract

Soursop leaves (Annona muricata L.) are known to contain various bioactive compounds with potential antioxidant properties. This study aimed to evaluate the antioxidant activity of soursop leaf extract using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and to determine its total phenolic content using the Folin–Ciocalteu method. Antioxidant activity was assessed at extract concentrations of 500 and 1000 ppm, with quercetin used as a positive control. The results showed that the soursop leaf extract exhibited very strong antioxidant activity, with an IC50 value of 9,486 ppm. The total phenolic content of the extract was relatively high, ranging from 102,929 to 195,702 mg GAE/g extract. The strong antioxidant activity observed is likely associated with the high content of phenolic compounds, which play a crucial role in scavenging free radicals. These findings suggest that soursop leaf extract has significant potential as a natural antioxidant source for applications in the functional food and pharmaceutical industries.
Co-Authors Dwi Kartika Wati Faisal Faisal Kammal Nizam Amrullah Majida Ramadhan Nour Athiroh Abdoes Sjakoer Nurul Jadid Mubarokati Salsabilatul Mukarommah