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All Journal Jurnal Ilmu Pertanian Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Jurnal Sains Peternakan Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Pharmasipha JURNAL ILMIAH PETERNAKAN TERPADU Journal of Dedicators Community Biota Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) JSMARTech : Journal of Smart Bioprospecting and Technology J-PEN Borneo : Jurnal Ilmu Pertanian Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Jurnal Riset Perikanan dan Kelautan Jurnal Sains Alami (Known Nature) MERDEKA: Jurnal Ilmiah Multidisiplin KOPEMAS Exhibition and Seminar on Science and Creative Technology – Al Azhar Proceeding Genbinesia Journal of Biology
Nurul Jadid Mubarokati
Universitas Islam Malang
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Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Mubarakati, Nurul Jadid; Aulanni'am, Aulanni'am; Sumitro, Sutiman Bambang; Ciptadi, Gatot
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Histopathological Profile of Therapeutic Doses of Mango Mistletoe Methanolic Extract (MMME) in Cardiac of Hypertensive Rats (DOCA-Salt) Hakimah, Annisatul Anis; Sjakoer, Nour Athiroh Abdoes; Mubarakati, Nurul Jadid
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 2 (2021)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2021.002.02.48

Abstract

This study determined the effect of Mango mistletoe Methanolic Extract (MMME) on the cardiac's histopathological profile of hypertensive rats (DOCA-Salt) treated. The MMME was treated on fifty rats for 14 days, divided into five treatments: negative control, positive control, doses 50, 100, and 200 mg/kg BW with five replications. One-way ANOVA analysis was used, namely JAMOVI with version 1.1.9.0, and for cell calculation, diameter using the IMAGE J application. Results showed that there were no significant differences in the histopathological profile of the cardiac in hypertensive rats (DOCA-Salt) treated with MMME for 14 days on the diameter of the heart muscle cells between the control (+) and control groups (-), treatments 1, 2, and 3. This is evidenced by the analysis of p-value> 0.05, namely 0.187 millimeters. Therefore, we concluded that MMME does not affect the diameter of heart/cardiac organ muscle cells. However, there was a significant difference in the amount of necrosis in the cardiac of hypertensive rats between control (+) and control (-) groups, treatments 1, 2, and 3. Based on the results, MMME reduces the number of necrosis in the heart/cardiac organ. 
Effects of Mango Mistletoe (Dendrophthoe pentandra L. Miq) Extracts on Brain in Hypertensive Rats Treated with Deoxycorticosterone Acetate (DOCA)-Salt Saputri, Syntia Diyah Hayu Ria; Sjakoer, Nour Athiroh Abdoes; Mubarakati, Nurul Jadid
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 2 (2021)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2021.002.02.55

Abstract

Mango mistletoe (Dendrophthoe pentandra) is a plant that has the potential as a medicinal plant. Mango mistletoe is reported to contain the flavonoids quercetin, mesoinositol, rutin, and tannins. Hypertension as a non-communicable disease is a very serious health problem experienced by people in developing countries. Hypertension can be caused by high ROS (reactive oxygen species) which can inhibit oxygen flow to the heart and brain. Antioxidants can reduce the risk of hypertension. Damage to the brain due to hypertension is manifest in the white matter area. This study aimed to see how the histopathological profile of the brain in hypertensive rats (DOCA-Salt) which were given mango mistletoe methanolic extract (MMME) by experimental method. The number of tested animals was 25 male Wistar rats divided into 5 groups, each group contained 5 rats, they were negative control (Control-) as normotension, positive control (Control+) as inducer, and the treatment group with the provision of MMME at a dose of 50 mg / kgBW, 100 mg / kgBW, and 200 mg / kgBW. Data were analyzed using JAMOVI 1.1.9.0. Based on the results of the study showed that the difference in significant values between all groups was p<0.05. So, that giving EMBM to male rat significantly can decrease the number of cell necrosis and affect the width of the white matter area of the brain. The three dose variations did not show a significant difference, so this effect was controlled by the MMME dose of 50 mg / KgBW which was the optimum dose in reducing the amount of cell necrosis in the white matter area of the male Wistar rat brain.
Role of Mango Mistletoe on Superoxide Dismutase (SOD) in Hypertensive Rats Exposed to DOCA-Salt Suroyya, Mariyam; Sjakoer, Nour Athiroh Abdoes; Mubarakati, Nurul Jadid
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 2 (2021)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2021.002.02.61

Abstract

Abstract: Hypertension is a condition where there is an abnormal rise in blood pressure that may be the primary cause of cardiovascular disease. Hypertension induces the production of free radicals known as reactive oxygen species (ROS) and oxidative stress. The purpose of this study is to further examine the function of Dendropthoe pentandra as an endogenous antioxidant modulator in this case superoxide dismutase (SOD) in hypertensive rats.The testing approach used is experimental. Data were analyzed using the one-way ANOVA test and the Post Hoc test to see variations in SOD levels in different treatments. This research used a hypertensive rat model induced by deoxycorticosterone acetate (DOCA) and salt. The number of animals tested was 25 white male rats divided into 5 groups, each containing 5 rats.The group consisted of a control group, a group of non- Extract methanolic of mango mistletoe hypertensive rats, and three groups of hypertensive rats receiving mango mistletoe methanolic extract (EMBM) at dosages of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight. Based on the results of the study, it is known that the levels of increased lung SOD with extract methanolic of mango mistletoe dosage variations in all treatment groups were not different. The administration of mango parasite methanolic extract at a dose of 50 mg / kgBW was optimum in increasing lung SOD levels in hypertensive rats.
In Silico Screening of Schleichera oleosa Phytocompounds as Estrogen Receptors Alpha Inhibitors for Breast Cancer Pratiwi, Radita Intan Aisyah; Rahayu, Tintrim; Mubarakati, Nurul Jadid
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 1 (2020)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2020.002.01.14

Abstract

Abstrak: This study aimed to predict the potential activity, toxicity, and interaction of fifteen bioactive compounds from Schleichera oleosa as estrogen receptors alpha inhibitors via in silico analysis. Active compound was downloaded from the PubChem database, and the 3D structure of the human estrogen receptor alpha (ERα) was obtained from the Protein Data Bank database with 4-Hydroxytamoxyfen as a positive control. The interaction of bioactive compounds with macromolecule was examined via a molecular specific docking using AutoDock Vina with PyRx 9.5 software. The protein was visualized using Discovery Studio 4.1. The drug-likeness property and human intestinal absorption of those fifteen bioactive compounds were evaluated through absorption, distribution, metabolism, and excretion (ADME) analysis using the pkCSM online tool program. The interactions between proteins and ligands are largely through the formation of hydrogen and van der Waals bonds. The binding energy of lupeol acetate, lupeol, schleicheol 1, betulinic acid, betulin, beta-sitosterol, schleicherastatin 7, schleicherastatin 2, schleicherastatin 4, scopoletin, schleicherastatin 3, schleicherastatin 1, schleicherastatin 6, schleicherastatin 5 alpha and schleicherastatin receptors including -8.3, -8.3, -7.1, -7.1, -6.7, -6.6, -6.6, -6.5, -6.5, -6.3, -6.2, -6.2 -6.1, -5.9 and -5.5 kcal / mol, respectively . The in silico ADME analysis also revealed that lupeol and lupeol acetate were the best active compound that passes the test based on the Lipinski rule, ADME, and toxicity. Therefore, it can be stated that Schleichera oleosa has potential as an inhibitor of alpha estrogen receptors. The inhibitory activity of alpha estrogen receptors has led to new breakthroughs in plant-based medicinal products, particularly for breast cancer. Keyword: Schleichera oleosa, alpha estrogen receptors, phytocompound, breast cancer and in silico
Pengaruh Pemberian Daun Pepaya (Carica papaya) Terhadap Endoparasit dan Hasil Panen Susu Sapi Perah (Bos taurus) sebagai Antihelmintik Alami di Balai Besar Pelatihan Peternakan (BBPP) Batu Nia Novita Nurrohmah; Nour Athiroh AS; Nurul Jadid Mubarakati
Jurnal SAINS ALAMI (Known Nature) Vol 3, No 2 (2021)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v3i2.7428

Abstract

Helmintiasis is a worm disease that often occurs in cows so that it can reduce milk harvesting. One prevention that can be done  with natural ingredients in the form of papaya leaf (Carica papaya). Papaya leaves contain alkaloids, enzyme papain, saponin, flavonoid, and tannin. The purpose of this experiment was to analyze the effect of papaya leaf (Carica papaya) on endoparasites and milk harvesting. This is an experimental research used a pre-test post-test control group design on five dairy cows with one treatment, specifically 70% basal green feed and 30% papaya leaves and one positive control (Albendazole). Method of identification use the qualitative method is, native method (direct slide) and the flotation method (Flotation method). The obtained data were analyzed with a Anova test, it showed that result is (p<0,05). That are able to suppress the growth of endoparasites in cow 1, 2, 3, 4, 5 by 60%, 58%, 50%, 45% , and 34% respectively in the native method and the floating method are 75%, 63%, 56%, 45%, and 34%. Increased harvesting milk for 3 weeks is 2-4 liters.  It was conluded that there was an influence between papaya leaves (Carica papaya) on endoparasites in dairy cows (Bos taurus) as a natural antihelmintic. Keywords: Helmintiasis, Papaya Leaf (Carica papaya), Endoparasite, Cows (Bos taurus), Milk Harvesting ResultABSTRAKHelmintiasis adalah penyakit cacingan yang sering terjadi pada sapi sehingga dapat menurunkan hasil panen susu. Salah satu pencegahan alami yang dapat dilakukan yakni dengan memberikan daun pepaya (Carica papaya) sebagai pengobatan alami. Daun pepaya merupakan bahan alami yang mengandung senyawa aktif berupa alkaloid, flavonoid, enzim papain, saponin, dan tanin yang memiliki efek antihelmintik pada sapi perah (Bos taurus). Tujuan dari penelitian ini adalah untuk menganalisa efek daun pepaya (Carica papaya) terhadap endoparasit dan hasil panen susu. Penelitian ini merupakan penelitian eksperimental, menggunakan rancangan penelitian pre test post test control group design pada 5 ekor sapi perah dengan 1 perlakuan yaitu pemberian pakan hijau basal 70% dan daun pepaya 30% dan 1 kontrol positif (Albendazole). Metode pelaksanaan penelitian menggunakan uji feses metode kualitatif yaitu metode natif (Direct slide) dan metode apung (Flotation method). Data yang diperoleh dianalisis dengan uji statistika (Anova) dengan hasil (p<0,05). Hasil penelitian ini menunjukkan bahwa sapi 1, 2, 3, 4, 5 mengalami penurunan endoparasit sebesar 60%, 58%, 50%, 45%, dan 34% secara berturut-turut pada metode natif dan metode apung sebesar 75%, 63%, 56%, 45%, dan 34%. Pemberian daun pepaya pada sapi perah selama 3 minggu dapat meningkatkan panen susu sebesar 2-4 per hari. Sehingga dapat disimpulkan bahwa terdapat pengaruh antara daun pepaya  (Carica papaya) terhadap endoparasit pada sapi perah (Bos taurus) dan hasil panen susu.Kata kunci: Helmintiasis, Daun Pepaya (Carica papaya), Endoparasit, Sapi (Bos taurus),  Hasil panen susu
Uji Toksisitas Ekstrak Metanolik Kombinasi Daun Benalu Teh Dan Daun Benalu Mangga Terhadap Profil Lipid Tikus Betina (Rattus norvegicus) pada Paparan Sub-Kronik 28 Hari Tengku Anggun Lestari; Nour Athiroh; Nurul Jadid Mubarakati
Jurnal SAINS ALAMI (Known Nature) Vol 3, No 1 (2020)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v3i1.6621

Abstract

Tea Benalu (Scurrula atropurpurea [Bl.] Dans.) and Mango Benalu (Dendrophthoe pentandra L. miq.) containssecondary metabolites with potential antioxidant potential. Antioxidants can inhibit the oxidation process and reduce Total Cholesterol, Triglycerides, Low-Density Lipoprotein (LDL) and increase High-Density Lipoprotein (HDL). The safety of the preparations for the combination of parasites of tea and mango leaves was tested to obtain toxic effects after repeated treatment tests over some time. The purpose of this study was to determine the toxic effects of a combination of methanolic extracts of a combination of tea parasites and mangoes in female rats (Rattus norvegicus) subchronically for 28 days by looking at the results of the clinical biochemical examination on lipid profiles, namely total cholesterol levels, triglycerides, high-density lipoproteins (28%). HDL), and Low-Density Lipoprotein (LDL). The method of this study was experimental with a combination dose of methanol extract of tea parasite leaves and mango parasite leaves at a dose of 250 mg / KgBB, 500 mg / KgBB and 1000 mg / KgBB with 5x replications each treatment. The subjects used white rats (Rattus norvegicus) female Wistar strains aged 6-8 weeks with a minimum body weight of 100 grams. The treatment in this study was in the form of each dose given 5 times a week for 28 days (subchronic toxicity test) orally. Lipid profile levels were analyzed using the one-way ANOVA test. The results showed that the level of control blood lipid profile with a treatment dose of 250 mg / KgBW, 500 mg / KgBW, and 1000 mg / KgBW was not significantly different. The toxicity test of combined extracts of tea leaves and mango parasite leaves of female rat Rattus novergicus on subchronic exposure 28 days with those doses showed no difference tangible between treatments of controls. This means it is safe and does not cause toxic properties in the lipid profile of female wistar rats.Keywords: Lipid Profile, Scurrula atropurpurea, Dendophthoe pentandra, Sub-Chronic Toxicity, Rattus norvegicus ABSTRAKBenalu Teh (Scurrula atropurpurea [Bl.] Dans.) dan  Benalu Mangga (Dendrophthoe pentandra L. miq.) mengandung metabolit sekunder berpotensi sebagai antioksidan. Antioksidan dapat memperhambat proses oksidasi dan menurunkan Cholesterol Total, Trigliserida, Low Density Lipoprotein (LDL) dan menaikkan High Density Lipoprotein (HDL). Keamanan sediaan kombinasi daun benalu teh dan mangga diuji memperoleh tentang efek toksik setelah uji perlakuan berulang dalam jangka waktu tertentu. Tujuan dari penelitian ini untuk mengetahui efek toksik kombinasi ekstrak metanolik kombinasi daun benalu teh dan mangga pada tikus betina (Rattus norvegicus) secara subkronik selama 28 hari dengan melihat hasil pemeriksaan biokimia klinis pada profil lipid, yaitu kadar Cholesterol Total, Trigliserida, High Density Lipoprotein (HDL), dan Low Density Lipoprotein (LDL). Metode penelitian ini adalah eksperimental dengan perlakuan dosis kombinasi ekstrak metanol daun benalu teh dan daun benalu mangga dosis 250 mg/KgBB, 500 mg/KgBB dan 1000 mg/KgBB dengan ulangan 5x tiap perlakuan. Subjek menggunakan tikus putih (Rattus norvegicus) betina strain wistar yang berumur 6-8 minggu dengan berat badan minimal 100 gram. Perlakuan dalam penelitian ini berupa setiap dosis  diberikan 5 kali seminggu selama 28 hari (uji toksisitas subkronik) secara per-oral. Kadar profil lipid dianalisis menggunakan uji One-way ANOVA. Hasil penelitian menunjukkan bahwa kadar profil lipid darah kontrol dengan perlakuan dosis 250 mg/KgBB, 500 mg/KgBB dan 1000 mg/KgBB tidak berbeda nyata. Uji”toksisitas”ekstrak kombinasi daun benalu teh dan daun benalu mangga  tikus betina Rattus novergicus pada paparan subkronik 28 hari dengan”dosis”tersebut menunjukkan hasil tidak”beda”nyata antara perlakuan terhadap”kontrol. ”Hal ini berarti aman dan tidak menimbulkan sifat toksik pada profil lipid tikus wistar betina.Kata Kunci : Profil Lipid, Scurrula atropurpurea, Dendophthoe pentandra, Subkronik, Rattus norvegicus
KARAKTERISASI PROFIL ZONA PELUSIDA MANUSIA (Homo sapiens) MENGGUNAKAN ANALISIS IN SILICO Nurul Jadid Mubarakati; Saimul Laili
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 2 No 2 (2017): Dunia Makhluk Hidup Mikro dan Makro
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.248 KB)

Abstract

Most important in mammlian fertilization were egg binding sperm, whose fusion generates a zygote that will develop into a new individual. In mammals, zona pellucida (ZP) is a specialized extracellular matrix of the egg. The matrix is important for this process by directly mediating species-restricted recognition between gametes. The aims of this study was to analyze the profile of human (Homo sapiens) zonna pellucida (hZP) using in silico method, position of O-linked and N-linked glycosilation units on human ZP amino acid sequence and to predict interaction between ZP and ADAM 2. The method of research was using in silico analysis. ZP protein profile was using data mining in UNIPROT database. Profile exploration searched primery funtion of protein, location, expression place, protein structure and ZP-interaction protein. The Primer data was compared each other. Determining O-linked and N-linkedglycosilation units in hZP amino acid sequence was analyzed using O and N-Glycosylation sites prediction. The results showed that in silico analysis conducted that profile of ZP3 identified as a primary receptor sites for spermatozoa and induced acrosome reaction. Amino acid sequence in ZP have potential site in N-Linked and O-linked glycosilation units, but have quantity different. Keywords : human zonna pellucida, N- Glycosylation, O- Glycosylation, in silico.ABSTRAKTahapan awal yang paling penting dalam fertilisasi mamalia adalah ikatan antara sel telur dan sperm yang dilanjutkan terbentuknya zigot dan terjadi perkembangan individu baru. Pada mamalia, zona pelusida merupakan lapisan ekstraseluler sel telur. Lapisan ini sangat penting dalam proses pengenalan secara langsung yang dimediasi oleh interaksi gamet. Tujuan dari penelitian ini adalah untuk mengetahui profil zona pelusida manusia secara in silico, untuk mengetahui posisi O-linked dan N-linked pada sekuen ZP manusia (Homo sapiens) serta memprediksi interaksi antara ZP dengan ADAM 2. Metode yang digunakan dalam penelitian ini adalah menggunakan analisis in silico.Profil Protein Zona pelusida dilakukan dengan cara data mining di database UNIPROT. Eksplorasi profil dilakukan pada pencarian fungsi utama protein, lokasi protein, tempat ekspresi, struktur protein,dan juga protein yang berinteraksi dengan zona pelusida. Informasi dasar tersebut kemudian dikomparasi agar diketahui peranan protein yang paling krusial. Penentuan sisi potensial O-linked dan N-linked pada sekuen ZP manusia dianalisis menggunakan O dan N-Glycosylationsites prediction. Interaksi ZP dengan ADAM 2 menggunakan moleculer docking. Hasil penelitian menunjukkan bahwa profil zona pelusida (ZP) secara in silico diperoleh bahwa ZP3 berperan sebagai reseptor primer yang berperan dalam ikatan dengan spermatozoa pada oosit dan menginduksi reaksi akrosom. ZP memiliki sisi potensial pada N-glikosilasi maupun O-glikosilasi, namun memiliki jumlah yang berbeda.Kata kunci: Zona Pelusida manusia, N-glikosilasi, O-glikosilasi, in silico
Studi Subkronik.28 Hari: Uji Toksisitas Ekstrak Metanolik Kombinasi Scurulla atropurpurea dan Dendrophthoe pentandra terhadap Kerusakan Fungsi Ginjal Tikus Wistar Betina Malia Anjani; Nour Athiroh AS; Nurul Jadid Mubarakati
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.141 KB)

Abstract

Mistletoe tea (Scurrula. atropurpurea (Bl.) Dans) and Mistletoe Mango (Dendrophthoe pentandra) are parasitic plants that live on tea and mango plants, very potential as medicines, containing several secondary metabolite compounds such as flavonoids (quercetin). Flavonoids can act as natural antioxidants that can protect the kidneys from free radicals. Damage to kidney function can indicate from creatinine, BUN, urea, and renal histopathology. The.purpose.of.this study was.to determine toxicity of. a combination of. Mistletoe tea. extract and mango mistletoe (EMBTBM) to damage kidney function in female Wistar rats for 28 days, using the true experimental design method. Data on creatinine, bun, urea, and renal histopathology data were analyzed using the ANOVA. test. The number of test animals is 20 female white rats divided into four groups; each group there are five rats. Group.1 a control, then groups 3, and 4. as. treatment. Based on the results of the study showed that statistically significant. between. all groups. was p> 0.05. Therefore,.EMBTBM given to female.rats for.28 days at a dose.of 250 mg/KgBB, 500.mg/KgBB, and.1000 mg/KgBB reduces levels of creatinine, bun, urea and renal cell necrosis, in this case, the administration of EMBTBM tends to be safe and does not cause rise toxic properties in damage to kidney function in female Wistar rats. Keywords: .Subchronic, kidney function, and extract. ABSTRAK Benalu teh (Scurrula atropurpurea (Bl.) Dans) dan Benalu Mangga (Dendrophthoe pentandra) merupakan tanaman parasit yang hidup.menumpang pada tanaman teh dan mangga, sangat berpotensi sebagai obat-obatan, karena mengandung senyawa flavonoid yaitu quercetin dan rutin. Flavonoid berperan sebagai penyedia. antioksidan. alami. yang. melindungi. ginjal dari zat radikal.bebas. Kerusakan.fungsi ginjal diindikasi dari. kadar. kreatinin, BUN, urea, dan histopataologi ginjal. Tujuan penelitian ini adalah mengetahui. toksisitas pemberian. kombinasi ekstrak. benalu. teh. dan benalu mangga (EMBTBM) terhadap kerusakan fungsi ginjal pada tikus wistar betina selama 28 hari, dengan metode true eksperimental design. Data kadar kreatinin, bun, urea, dan histopatologi ginjal dianalisis menggunakan uji ANOVA. Penelitian ini menggunakan hewan.coba.berjumlah. 20. ekor. tikus. putih. betina. Hewan coba dibagi. menjadi 4.kelompok denga 5 ekor tikus pada tiap kelompok. Kelompok.1sebagai. kontrol, sementara kelompok. 3 dan 4 diberi perlakuan EMBTBM. Hasil. penelitian. menunjukan bahwa. perbedaan. nilai signifikan. antara. semua. kelompok. yaitu p>0.05..EMBTBM yang disondekan pada tikus wistar betina.selama 28.hari. dengan. dosis yang diberikan yaitu 250. mg/KgBB, 500. mg/KgB, dan 1000. mg/KgBB menurunkan kadar kreatinin, bun, urea dan nekrosis sel ginjal, dalam hal ini pemberian EMBTBM cenderung aman dan tidak menimbulkan sifat toksik pada kerusakan fungsi ginjal tikus wistar betina. Kata Kunci. : Ekstrak, Fungsi ginjal, dan Subkronik.
Uji Toksisitas Subkronik 28 Hari Kombinasi Ekstrak Metanolik Benalu Teh dan Benalu Mangga Terhadap Profil Protein pada Tikus Wistar (Rattus norvegicus) Betina Umu Intan Kinasih; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.375 KB)

Abstract

There are 3 indicators for indicating a protein breakdown in the blood through a total protein, albumin, and globulin stability. A compound capable of acting as antioxidants is needed to stabilize the protein's condition. This research is aimed at identifying the toxic effects of extract’s combination from tea parasite and mango parasite on the conditions of blood’s protein by giving EMBTBM constantly for 28 days. The data analysis used ANOVA one-way by SPSS version 17.0. As for the animal test used was a female Wistar rat of 20 with four treatments are control, treatment 1 with a dose of 250mg/Kg body weight (BW). Treatment 2 with a dose of 500mg/Kg body weight (BW) and treatment 3 with a dose of 1000mg/Kg body weight (BW). The result of this research shows that there is no difference between treatment and control over the results of a total protein and albumin. But, at the result of globulin, there is a difference between treatment and control. Therefore, EMBTBM is safe for profile protein and does not cause toxicity because protein is stable and not over-reducing. Keywords:Subchronic,Total Proteins, Albumine, Globuline, Toxicity
Co-Authors Abdul Hamid A. Toha Adi Sofyan Ansori, Muhammad Agus Mohammad Hikam Ahmad Taufiq Aisyah Febria Damayanti Alik Maulidiyah Annisatul Hakimah Ari Hayati Ari Hayati Arifiati, Shinta Arina Roikhana Asari, Devi Indrayani Fitri Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Azis Mawardi Azis Mawardi Chintya Ayu Pertiwi Della Aljanna Savita Dewi Qurrot &#039;Aini Dewi, Atikah Nur Shabrina Razan Dyan Aprillia Susanti Eka Kumalasari Fradina, IntanTrixi Galuh Ayu Setya Sari Gatot Ciptadi Gatra Ervi Jayanti Gatra Ervi Jayanti Hasan Zayadi Hatif Khusnin Nida' Hikam, Agus Mohammad Husain Latuconsina Islami, Darajatun Islamya, Nur Afni Auli Ismatud Diniyah ISNAWATI Istirochah Pujiwati Januarista, Terra Janur Annisa Sajida Kamilah, Nahdhiyatun Kharisma, Viol Dhea Kurniasari, Yulia Lestari, Tengku Anggun M. Abdul Qodir Jailani Ma'ruf, Muhammad Maghfiroh, Faldha Laili Malia Anjani Moch Mustakim Moch Mustakim Muhammad Dailami, Muhammad Musaffak, Taufik Ridwan Muzayyan, Abdullah Rozzen Nabilah Miftachul Khoiriyah Nabilla Abidah Alfita Nafisa Nafisa Nia Novita Nurrohmah Nour Athiroh Abdoes Sjakoer Nur Cahya Kartika Slamet Nur Laily Azizah Nur Mufida Nur Mufida Nurrohmah, Nia Novita Nurul Humaidah Oktavia Rahayu Puspitarini Oktaviana, Nimas Ajeng Pinanti, Honesty Nurizza Pratiwi, Radita Intan Aisyah Radita Intan Aisyah Pratiwi Rafsanjani, Ramizard Rahayu Dwi Lestari Ramadhan, Majida Rasyadan Taufiq Probojati Rossa Martha Wulandari Rossa Martha Wulandari Saimul Laili Sama’ Iradat Tito Saputri, Syntia Diyah Hayu Ria Silvia, Putri Azizatul Siti Khoirun Ni’mah Siti Qurrotul Aini Sulis Setiawati Suroyya, Mariyam Sutiman B. Sumitro Sutiman Bambang Sumitro Syaiful Arifin Tengku Anggun Lestari Thiya Fathiyatul Fauziyah Tintrim Rahayu, Tintrim Ula, Erfa Navadiatul Umu Intan Kinasih Vikriansyah, Muchammad Faruq Vina Fitria Wulandari Waliyyul Ahdi Alhikami Widyananda, Muhammad Hermawan Yunani, Silvi